LOCUS       BBa_K346084             2863 bp    DNA     linear       25-Nov-2024
DEFINITION  T7 promoter(BBa_I719005)+RBS(B0032)+T3 RNA polymerase+Terminator(B0
            015)
ACCESSION   BBa_K346084
VERSION     BBa_K346084.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     RBS             32..44
                     /label=RBS-3\Weak
     RBS             38..41
                     /label=RBS
     misc_feature    32..44
                     /label=BBa_B0032
     RBS             32..44
                     /label=BBa_B0032
     CDS             71..2726
                     /label=t3 pol
     RBS             53..66
                     /label=b0032
     misc_feature    53..2726
                     /label=BBa_K346000
     stem_loop       2746..2789
                     /label=stem_loop
     misc_feature    2735..2814
                     /label=BBa_B0010
     terminator      2735..2814
                     /label=BBa_B0010
     stem_loop       2830..2849
                     /label=T7 TE
     misc_feature    2856..2856
                     /label=stop
     polyA_site      2850..2863
                     /label=polya
     misc_feature    2823..2863
                     /label=BBa_B0012
     terminator      2823..2863
                     /label=BBa_B0012
     terminator      2735..2863
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg agatactaga gtcacacagg aaagtactag agtcacacag
       61 gaaagtacta gatgaacatc atcgaaaaca tcgaaaagaa tgacttctca gaaatcgaac
      121 tggctgctat cccgttcaac acactggctg accactacgg aagcgccttg gctaaagagc
      181 agttggcttt agaacatgag tcttatgagc taggcgagcg ccgcttcctc aagatgcttg
      241 agcgtcaagc gaaagctggt gagattgcag acaacgcagc cgctaagccg ttactcgcta
      301 cgcttctccc taagttaacc acacgtatcg tcgagtggct cgaagagtac gcatcgaaga
      361 aaggccgcaa gcctagcgca tacgcaccgc tccagttact caagccggag gcctccgcgt
      421 ttatcaccct gaaagttatc cttgcgtcac taaccagtac gaacatgaca accattcagg
      481 ccgctgctgg tatgctgggg aaagccattg aggacgaggc acgatttggg cgcatccgtg
      541 acctagaagc gaagcacttc aagaagcacg ttgaggaaca gcttaacaag cgccacgggc
      601 aagtctacaa gaaagcattt atgcaggtgg tcgaggccga tatgattggt cgaggtctgc
      661 ttggtggcga ggcgtggtct agctgggata aagaaaccac gatgcacgta gggattcgcc
      721 tgattgaaat gctgattgaa tccacgggtc tggtggaatt acagcgccac aacgcaggta
      781 acgcaggctc tgaccatgag gcactgcaac tggcccaaga gtacgtggac gtattagcga
      841 agcgtgcagg cgctctggcg ggtatctctc cgatgttcca gccgtgtgtc gtaccgccga
      901 aaccttgggt agcaatcaca gggggcggct attgggctaa cggtcgcaga cctttggcac
      961 tcgttcgcac tcactctaag aagggcttga tgcgctacga agacgtttac atgccagaag
     1021 tctacaaggc tgtgaacctc gcgcaaaaca ccgcatggaa aatcaacaag aaagttcttg
     1081 ctgttgtcaa tgagattgtt aactggaaga attgcccggt agcagacatt ccatcgctgg
     1141 agcgccaaga gttaccgcct aagcctgacg acattgacac caacgaggca gcgctcaagg
     1201 agtggaagaa agccgctgct ggtatctatc gcttggacaa ggcacgagtg tctcgccgta
     1261 tcagcttaga gttcatgctg gagcaggcca acaagttcgc aagtaagaaa gcaatctggt
     1321 tcccttacaa catggactgg cgcggtcgtg tgtacgctgt gccgatgttc aacccgcaag
     1381 gcaacgacat gacgaaaggt ctgctgaccc ttgctaaagg caagccaatc ggtgaggaag
     1441 gtttctactg gctgaaaatc cacggtgcga actgtgcggg tgttgataag gttccattcc
     1501 cggagcgcat cgcgttcatt gagaagcacg tagacgacat tctggcttgc gctaaagacc
     1561 caatcaataa cacttggtgg gctgagcagg attcaccgtt ctgtttcctc gcgttttgct
     1621 tcgagtatgc aggcgttacg caccacggtc tgagctacaa ttgctctctg ccgctggcgt
     1681 tcgacgggtc ttgctctggt atccagcact tctccgcgat gctccgcgat gaggtaggcg
     1741 gtcgtgcggt taacctgctg ccaagcgaaa ccgtgcagga catttacggc atcgttgcac
     1801 agaaagtaaa cgagattctc aaacaggatg caatcaacgg cacgcctaac gagatgatta
     1861 ccgtgaccga caaggacacc ggggaaatct cagagaagct caaacttgga acctcaacgc
     1921 tggcgcaaca gtggctggca tatggtgtaa cccgtagcgt aactaaacgt tcggtcatga
     1981 cgctggctta cggttccaag gagttcggct ttcgtcaaca ggtattggat gacaccattc
     2041 agcctgcaat tgacagcggt aagggcttga tgttcaccca accgaaccaa gcggctggct
     2101 atatggctaa gctgatttgg gatgcggtaa gcgtgaccgt agttgcagcg gttgaggcga
     2161 tgaactggct caaatctgcc gctaagctgc tggctgctga ggtcaaggac aagaagacca
     2221 aggagattct gcgccaccgt tgcgcggttc actggactac gccggacggc ttcccggtct
     2281 ggcaggaata ccgcaagcca ctccagaagc gtctcgatat gattttctta gggcaattcc
     2341 gtctgcaacc gacgattaat accctcaagg attcaggcat tgacgcacac aagcaggagt
     2401 ctggcatcgc tcctaacttt gttcactcac aggacggtag ccacctccgc atgacagtcg
     2461 tttatgctca cgagaagtat ggcattgagt cctttgcgct catccatgac agctttggga
     2521 ctatcccggc agacgctggt aagctcttta aggctgtgcg tgaaacgatg gttatcacct
     2581 atgagaacaa cgatgtgctg gcagacttct actctcagtt tgccgaccag ctacacgaga
     2641 cccaactgga caagatgcct ccgcttccga agaaaggaaa cctgaacctg caagacattc
     2701 tcaagtctga ctttgccttt gcataatact agagccaggc atcaaataaa acgaaaggct
     2761 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2821 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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