BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K346000 1 BBa_K346000 RBS(B0032)+T3 DNA-directed RNA polymerase 2010-09-24T11:00:00Z 2015-05-08T01:12:08Z Tn21 Released HQ 2013 This part is the coding region of MerR, which is a mercury responsive regulator from Tn21. false false _457_ 0 4411 9 In stock true We cloned this part from plasmid NR1, which was a gift from Anne O. Summers. false Haoqian Zhang and Weiye Wang annotation2092860 1 b0032 range2092860 1 1 14 annotation2092859 1 t3 pol range2092859 1 19 2674 BBa_K346084 1 BBa_K346084 T7 promoter(BBa_I719005)+RBS(B0032)+T3 RNA polymerase+Terminator(B0015) 2010-10-18T11:00:00Z 2015-05-08T01:12:10Z T7 promoter(BBa_I719005)+RBS(B0032)+T3 RNA polymerase+Terminator(B0015) T7 promoter(BBa_I719005)+RBS(B0032)+T3 RNA polymerase+Terminator(B0015) false false _457_ 0 5848 9 It's complicated false T7 promoter(BBa_I719005)+RBS(B0032)+T3 RNA polymerase+Terminator(B0015) false Weiye Wang component2092892 1 BBa_K346000 component2092886 1 BBa_I719005 component2092899 1 BBa_B0015 component2092888 1 BBa_B0032 annotation2092886 1 BBa_I719005 range2092886 1 1 23 annotation2092892 1 BBa_K346000 range2092892 1 53 2726 annotation2092888 1 BBa_B0032 range2092888 1 32 44 annotation2092899 1 BBa_B0015 range2092899 1 2735 2863 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K346000_sequence 1 tcacacaggaaagtactagatgaacatcatcgaaaacatcgaaaagaatgacttctcagaaatcgaactggctgctatcccgttcaacacactggctgaccactacggaagcgccttggctaaagagcagttggctttagaacatgagtcttatgagctaggcgagcgccgcttcctcaagatgcttgagcgtcaagcgaaagctggtgagattgcagacaacgcagccgctaagccgttactcgctacgcttctccctaagttaaccacacgtatcgtcgagtggctcgaagagtacgcatcgaagaaaggccgcaagcctagcgcatacgcaccgctccagttactcaagccggaggcctccgcgtttatcaccctgaaagttatccttgcgtcactaaccagtacgaacatgacaaccattcaggccgctgctggtatgctggggaaagccattgaggacgaggcacgatttgggcgcatccgtgacctagaagcgaagcacttcaagaagcacgttgaggaacagcttaacaagcgccacgggcaagtctacaagaaagcatttatgcaggtggtcgaggccgatatgattggtcgaggtctgcttggtggcgaggcgtggtctagctgggataaagaaaccacgatgcacgtagggattcgcctgattgaaatgctgattgaatccacgggtctggtggaattacagcgccacaacgcaggtaacgcaggctctgaccatgaggcactgcaactggcccaagagtacgtggacgtattagcgaagcgtgcaggcgctctggcgggtatctctccgatgttccagccgtgtgtcgtaccgccgaaaccttgggtagcaatcacagggggcggctattgggctaacggtcgcagacctttggcactcgttcgcactcactctaagaagggcttgatgcgctacgaagacgtttacatgccagaagtctacaaggctgtgaacctcgcgcaaaacaccgcatggaaaatcaacaagaaagttcttgctgttgtcaatgagattgttaactggaagaattgcccggtagcagacattccatcgctggagcgccaagagttaccgcctaagcctgacgacattgacaccaacgaggcagcgctcaaggagtggaagaaagccgctgctggtatctatcgcttggacaaggcacgagtgtctcgccgtatcagcttagagttcatgctggagcaggccaacaagttcgcaagtaagaaagcaatctggttcccttacaacatggactggcgcggtcgtgtgtacgctgtgccgatgttcaacccgcaaggcaacgacatgacgaaaggtctgctgacccttgctaaaggcaagccaatcggtgaggaaggtttctactggctgaaaatccacggtgcgaactgtgcgggtgttgataaggttccattcccggagcgcatcgcgttcattgagaagcacgtagacgacattctggcttgcgctaaagacccaatcaataacacttggtgggctgagcaggattcaccgttctgtttcctcgcgttttgcttcgagtatgcaggcgttacgcaccacggtctgagctacaattgctctctgccgctggcgttcgacgggtcttgctctggtatccagcacttctccgcgatgctccgcgatgaggtaggcggtcgtgcggttaacctgctgccaagcgaaaccgtgcaggacatttacggcatcgttgcacagaaagtaaacgagattctcaaacaggatgcaatcaacggcacgcctaacgagatgattaccgtgaccgacaaggacaccggggaaatctcagagaagctcaaacttggaacctcaacgctggcgcaacagtggctggcatatggtgtaacccgtagcgtaactaaacgttcggtcatgacgctggcttacggttccaaggagttcggctttcgtcaacaggtattggatgacaccattcagcctgcaattgacagcggtaagggcttgatgttcacccaaccgaaccaagcggctggctatatggctaagctgatttgggatgcggtaagcgtgaccgtagttgcagcggttgaggcgatgaactggctcaaatctgccgctaagctgctggctgctgaggtcaaggacaagaagaccaaggagattctgcgccaccgttgcgcggttcactggactacgccggacggcttcccggtctggcaggaataccgcaagccactccagaagcgtctcgatatgattttcttagggcaattccgtctgcaaccgacgattaataccctcaaggattcaggcattgacgcacacaagcaggagtctggcatcgctcctaactttgttcactcacaggacggtagccacctccgcatgacagtcgtttatgctcacgagaagtatggcattgagtcctttgcgctcatccatgacagctttgggactatcccggcagacgctggtaagctctttaaggctgtgcgtgaaacgatggttatcacctatgagaacaacgatgtgctggcagacttctactctcagtttgccgaccagctacacgagacccaactggacaagatgcctccgcttccgaagaaaggaaacctgaacctgcaagacattctcaagtctgactttgcctttgcataa BBa_B0032_sequence 1 tcacacaggaaag BBa_K346084_sequence 1 taatacgactcactatagggagatactagagtcacacaggaaagtactagagtcacacaggaaagtactagatgaacatcatcgaaaacatcgaaaagaatgacttctcagaaatcgaactggctgctatcccgttcaacacactggctgaccactacggaagcgccttggctaaagagcagttggctttagaacatgagtcttatgagctaggcgagcgccgcttcctcaagatgcttgagcgtcaagcgaaagctggtgagattgcagacaacgcagccgctaagccgttactcgctacgcttctccctaagttaaccacacgtatcgtcgagtggctcgaagagtacgcatcgaagaaaggccgcaagcctagcgcatacgcaccgctccagttactcaagccggaggcctccgcgtttatcaccctgaaagttatccttgcgtcactaaccagtacgaacatgacaaccattcaggccgctgctggtatgctggggaaagccattgaggacgaggcacgatttgggcgcatccgtgacctagaagcgaagcacttcaagaagcacgttgaggaacagcttaacaagcgccacgggcaagtctacaagaaagcatttatgcaggtggtcgaggccgatatgattggtcgaggtctgcttggtggcgaggcgtggtctagctgggataaagaaaccacgatgcacgtagggattcgcctgattgaaatgctgattgaatccacgggtctggtggaattacagcgccacaacgcaggtaacgcaggctctgaccatgaggcactgcaactggcccaagagtacgtggacgtattagcgaagcgtgcaggcgctctggcgggtatctctccgatgttccagccgtgtgtcgtaccgccgaaaccttgggtagcaatcacagggggcggctattgggctaacggtcgcagacctttggcactcgttcgcactcactctaagaagggcttgatgcgctacgaagacgtttacatgccagaagtctacaaggctgtgaacctcgcgcaaaacaccgcatggaaaatcaacaagaaagttcttgctgttgtcaatgagattgttaactggaagaattgcccggtagcagacattccatcgctggagcgccaagagttaccgcctaagcctgacgacattgacaccaacgaggcagcgctcaaggagtggaagaaagccgctgctggtatctatcgcttggacaaggcacgagtgtctcgccgtatcagcttagagttcatgctggagcaggccaacaagttcgcaagtaagaaagcaatctggttcccttacaacatggactggcgcggtcgtgtgtacgctgtgccgatgttcaacccgcaaggcaacgacatgacgaaaggtctgctgacccttgctaaaggcaagccaatcggtgaggaaggtttctactggctgaaaatccacggtgcgaactgtgcgggtgttgataaggttccattcccggagcgcatcgcgttcattgagaagcacgtagacgacattctggcttgcgctaaagacccaatcaataacacttggtgggctgagcaggattcaccgttctgtttcctcgcgttttgcttcgagtatgcaggcgttacgcaccacggtctgagctacaattgctctctgccgctggcgttcgacgggtcttgctctggtatccagcacttctccgcgatgctccgcgatgaggtaggcggtcgtgcggttaacctgctgccaagcgaaaccgtgcaggacatttacggcatcgttgcacagaaagtaaacgagattctcaaacaggatgcaatcaacggcacgcctaacgagatgattaccgtgaccgacaaggacaccggggaaatctcagagaagctcaaacttggaacctcaacgctggcgcaacagtggctggcatatggtgtaacccgtagcgtaactaaacgttcggtcatgacgctggcttacggttccaaggagttcggctttcgtcaacaggtattggatgacaccattcagcctgcaattgacagcggtaagggcttgatgttcacccaaccgaaccaagcggctggctatatggctaagctgatttgggatgcggtaagcgtgaccgtagttgcagcggttgaggcgatgaactggctcaaatctgccgctaagctgctggctgctgaggtcaaggacaagaagaccaaggagattctgcgccaccgttgcgcggttcactggactacgccggacggcttcccggtctggcaggaataccgcaagccactccagaagcgtctcgatatgattttcttagggcaattccgtctgcaaccgacgattaataccctcaaggattcaggcattgacgcacacaagcaggagtctggcatcgctcctaactttgttcactcacaggacggtagccacctccgcatgacagtcgtttatgctcacgagaagtatggcattgagtcctttgcgctcatccatgacagctttgggactatcccggcagacgctggtaagctctttaaggctgtgcgtgaaacgatggttatcacctatgagaacaacgatgtgctggcagacttctactctcagtttgccgaccagctacacgagacccaactggacaagatgcctccgcttccgaagaaaggaaacctgaacctgcaagacattctcaagtctgactttgcctttgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z