LOCUS BBa_K346085 2735 bp DNA linear 26-Nov-2024 DEFINITION Ptet-RBS-T7polymerase ACCESSION BBa_K346085 VERSION BBa_K346085.1 FEATURES Location/Qualifiers RBS 75..80 /label=rbs misc_feature 5..8 /label=conserved CDS 81..2735 /label=T7polymerase promoter 1..74 /label=Ptet ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaacacga ttaacatcgc taagaacgac ttctctgaca 121 tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc 181 gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga 241 tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca 301 tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag 361 ctaagcgcgg caagcgcccg acagccttcc agttcctgca agaaatcaag ccggaagccg 421 tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg 481 ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta 541 tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg 601 tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg 661 gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag 721 tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg 781 ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta 841 tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc 901 ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc 961 tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc 1021 ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag 1081 tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg 1141 cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc 1201 tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc 1261 gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca 1321 tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc 1381 cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta 1441 aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc 1501 cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta 1561 agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt 1621 tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc 1681 tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg 1741 taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg 1801 ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag 1861 tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta 1921 aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag 1981 tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata 2041 ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg 2101 ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg 2161 aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga 2221 agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc 2281 ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc 2341 agttccgctt acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac 2401 aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga 2461 ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct 2521 tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg 2581 acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc 2641 acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg 2701 acatcttaga gtcggacttc gcgttcgcgt aataa //