LOCUS       BBa_K346090             3428 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive promoter BBa_J23103 + CrtEBI(BBa_K274100)
ACCESSION   BBa_K346090
VERSION     BBa_K346090.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23103
     RBS             44..47
                     /label=BBa_K118012
     CDS             54..965
                     /label=BBa_I742151
     misc_feature    974..976
                     /label=SacI
     RBS             977..983
                     /label=rbs
     RBS             974..983
                     /label=BBa_J15001
     CDS             990..1919
                     /label=crtB coding sequence
     CDS             990..1922
                     /label=BBa_K118002
     misc_feature    1931..1933
                     /label=SacI
     RBS             1934..1940
                     /label=rbs
     RBS             1931..1940
                     /label=BBa_J15001
     misc_feature    2424..2429
                     /label=PstI site removed
     misc_feature    2835..2840
                     /label=PstI site removed
     CDS             1947..3425
                     /label=crtI coding sequence
     CDS             1947..3428
                     /label=BBa_K118003
     misc_feature    44..3428
                     /label=BBa_K274100
ORIGIN
        1 ctgatagcta gctcagtcct agggattatg ctagctacta gaggaggtac tagatgacgg
       61 tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc ggagcagtta ctggctgata
      121 ttgatcgacg ccttgatcag ttattgcccg tggagggaga acgggatgtt gtgggtgccg
      181 cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg ccccatgttg ctgttgctga
      241 ccgcccgcga tctgggttgc gctgtcagcc atgacggatt actggatttg gcctgtgcgg
      301 tggaaatggt ccacgcggct tcgctgatcc ttgacgatat gccctgcatg gacgatgcga
      361 agctgcggcg cggacgccct accattcatt ctcattacgg agagcatgtg gcaatactgg
      421 cggcggttgc cttgctgagt aaagcctttg gcgtaattgc cgatgcagat ggcctcacgc
      481 cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc catcggcatg caaggattgg
      541 ttcagggtca gttcaaggat ctgtctgaag gggataagcc gcgcagcgct gaagctattt
      601 tgatgacgaa tcactttaaa accagcacgc tgttttgtgc ctccatgcag atggcctcga
      661 ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca tcgtttttca cttgatcttg
      721 gtcaggcatt tcaactgctg gacgatttga ccgatggcat gaccgacacc ggtaaggata
      781 gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt aggcccgagg gcggttgaag
      841 aacgtctgag acaacatctt cagcttgcca gtgagcatct ctctgcggcc tgccaacacg
      901 ggcacgccac tcaacatttt attcaggcct ggtttgacaa aaaactcgct gccgtcagtt
      961 aataatacta gagctcaagg aggtactaga tgaataatcc gtcgttactc aatcatgcgg
     1021 tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc ctcaaagtta tttgatgcaa
     1081 aaacccggcg cagcgtactg atgctctacg cctggtgccg ccattgtgac gatgttattg
     1141 acgatcagac gctgggcttt caggcccggc agcctgcctt acaaacgccc gaacaacgtc
     1201 tgatgcaact tgagatgaaa acgcgccagg cctatgcagg atcgcagatg cacgaaccgg
     1261 cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat cgccccggct tacgcgtttg
     1321 atcatctgga aggcttcgcc atggatgtac gcgaagcgca atacagccaa ctggatgata
     1381 cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt gatgatggcg caaatcatgg
     1441 gcgtgcggga taacgccacg ctggaccgcg cctgtgacct tgggctggca tttcagttga
     1501 ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg ccgctgttat ctgccggcaa
     1561 gctggctgga gcatgaaggt ctgaacaaag agaattatgc ggcacctgaa aaccgtcagg
     1621 cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga accttactat ttgtctgcca
     1681 cagccggcct ggcagggttg cccctgcgtt ccgcctgggc aatcgctacg gcgaagcagg
     1741 tttaccggaa aataggtgtc aaagttgaac aggccggtca gcaagcctgg gatcagcggc
     1801 agtcaacgac cacgcccgaa aaattaacgc tgctgctggc cgcctctggt caggccctta
     1861 cttcccggat gcgggctcat cctccccgcc ctgcgcatct ctggcagcgc ccgctctaat
     1921 aatactagag ctcaaggagg tactagatga aaccaactac ggtaattggt gcaggcttcg
     1981 gtggcctggc actggcaatt cgtctacaag ctgcggggat ccccgtctta ctgcttgaac
     2041 aacgtgataa acccggcggt cgggcttatg tctacgagga tcaggggttt acctttgatg
     2101 caggcccgac ggttatcacc gatcccagtg ccattgaaga actgtttgca ctggcaggaa
     2161 aacagttaaa agagtatgtc gaactgctgc cggttacgcc gttttaccgc ctgtgttggg
     2221 agtcagggaa ggtctttaat tacgataacg atcaaacccg gctcgaagcg cagattcagc
     2281 agtttaatcc ccgcgatgtc gaaggttatc gtcagtttct ggactattca cgcgcggtgt
     2341 ttaaagaagg ctatctaaag ctcggtactg tccctttttt atcgttcaga gacatgcttc
     2401 gcgccgcacc tcaactggcg aaactgcaag catggagaag cgtttacagt aaggttgcca
     2461 gttacatcga agatgaacat ctgcgccagg cgttttcttt ccactcgctg ttggtgggcg
     2521 gcaatccctt cgccacctca tccatttata cgttgataca cgcgctggag cgtgagtggg
     2581 gcgtctggtt tccgcgtggc ggcaccggcg cattagttca ggggatgata aagctgtttc
     2641 aggatctggg tggcgaagtc gtgttaaacg ccagagtcag ccatatggaa acgacaggaa
     2701 acaagattga agccgtgcat ttagaggacg gtcgcaggtt cctgacgcaa gccgtcgcgt
     2761 caaatgcaga tgtggttcat acctatcgcg acctgttaag ccagcaccct gccgcggtta
     2821 agcagtccaa caaactgcaa actaagcgca tgagtaactc tctgtttgtg ctctattttg
     2881 gtttgaatca ccatcatgat cagctcgcgc atcacacggt ttgtttcggc ccgcgttacc
     2941 gcgagctgat tgacgaaatt tttaatcatg atggcctcgc agaggacttc tcactttatc
     3001 tgcacgcgcc ctgtgtcacg gattcgtcac tggcgcctga aggttgcggc agttactatg
     3061 tgttggcgcc ggtgccgcat ttaggcaccg cgaacctcga ctggacggtt gaggggccaa
     3121 aactacgcga ccgtattttt gcgtaccttg agcagcatta catgcctggc ttacggagtc
     3181 agctggtcac gcaccggatg tttacgccgt ttgattttcg cgaccagctt aatgcctatc
     3241 atggctcagc cttttctgtg gagcccgttc ttacccagag cgcctggttt cggccgcata
     3301 accgcgataa aaccattact aatctctacc tggtcggcgc aggcacgcat cccggcgcag
     3361 gcattcctgg cgtcatcggc tcggcaaaag cgacagcagg tttgatgctg gaggatctga
     3421 tataataa
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