LOCUS       BBa_K352010              980 bp    DNA     linear       22-Dec-2024
DEFINITION  pCooF Promoter coupled with RBS+GFP+Double terminator
ACCESSION   BBa_K352010
VERSION     BBa_K352010.1  
FEATURES             Location/Qualifiers
     misc_feature    8..33
                     /label=A+T rich sequence
     misc_feature    41..44
                     /label=CooA binding site
     misc_feature    52..56
                     /label=CooA binding site
     misc_feature    79..84
                     /label=-10 Region
     misc_feature    92..93
                     /label=Transcription Start
     misc_feature    36..61
                     /label=Protected by CooA
     misc_feature    23..91
                     /label=Protected by CooA+RNAP
     promoter        1..94
                     /label=BBa_K352002
     RBS             103..117
                     /label=RBS-1\Strong
     RBS             110..114
                     /label=RBS
     misc_feature    103..117
                     /label=BBa_B0030
     RBS             103..117
                     /label=BBa_B0030
     CDS             124..843
                     /label=GFP protein
     CDS             124..843
                     /label=BBa_E0040
     stem_loop       863..906
                     /label=stem_loop
     misc_feature    852..931
                     /label=BBa_B0010
     terminator      852..931
                     /label=BBa_B0010
     stem_loop       947..966
                     /label=T7 TE
     misc_feature    973..973
                     /label=stop
     polyA_site      967..980
                     /label=polya
     misc_feature    940..980
                     /label=BBa_B0012
     terminator      940..980
                     /label=BBa_B0012
     misc_feature    103..980
                     /label=BBa_E0840
ORIGIN
        1 ttcggcgtct tttcataccc ccataaaaac tctggataac tgtcatctgg ccgacagacg
       61 gggccgggct ttttgtcgct tactcggcgc caggtactag agattaaaga ggagaaatac
      121 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      181 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      241 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      301 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      361 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      421 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      481 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      541 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      601 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      661 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      721 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      781 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
      841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      961 tgggcctttc tgcgtttata
//