LOCUS       BBa_K352013              888 bp    DNA     linear       26-Nov-2024
DEFINITION  CooA coupled with pTetR+RBS+Double Terminator
ACCESSION   BBa_K352013
VERSION     BBa_K352013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start Codon
     CDS             1..669
                     /label=BBa_K352001
     misc_feature    678..696
                     /label=TetR 1
     misc_feature    678..731
                     /label=BBa_R0040
     promoter        697..702
                     /label=-35
     misc_feature    703..721
                     /label=TetR 2
     promoter        720..725
                     /label=-10
     promoter        678..731
                     /label=BBa_R0040
     misc_feature    744..747
                     /label=conserved
     RBS             740..751
                     /label=BBa_B0034
     stem_loop       771..814
                     /label=stem_loop
     misc_feature    760..839
                     /label=BBa_B0010
     terminator      760..839
                     /label=BBa_B0010
     stem_loop       855..874
                     /label=T7 TE
     misc_feature    881..881
                     /label=stop
     polyA_site      875..888
                     /label=polya
     misc_feature    848..888
                     /label=BBa_B0012
     terminator      848..888
                     /label=BBa_B0012
ORIGIN
        1 atgccaccac gcttcaatat tgcaaacgta ctgctgtctc ctgatggtga gacgttcttc
       61 cgcggttttc gctctaagat tcatgcgaaa ggctctctgg tatgtactgg tgaaggtgat
      121 gaaaacggtg tttttgttgt ggttgatggt cgcctgcgtg tttacctggt tggtgaggag
      181 cgtgagatta gcctgttcta cctgacttcc ggcgatatgt tctgcatgca ttccggctgc
      241 ctggttgaag ccaccgagcg caccgaagtg cgtttcgccg atatccgcac gttcgagcag
      301 aaactgcaaa cctgtccgtc tatggcatgg ggcctgatcg ccattctggg ccgtgctctg
      361 acctcctgta tgcgtaccat cgaagacctg atgttccacg atattaaaca acgtatcgcg
      421 ggctttttca tcgaccacgc taacactacc ggtcgccaga ctcagggtgg cgtaattgtt
      481 tctgttgact tcactgtaga ggaaatcgct aatctgatcg gtagctcccg ccagactact
      541 agcacggcgc tgaactctct gattaaagag ggttacatct cccgccaggg ccgtggtcac
      601 tatactatcc cgaacctggt tcgcctgaag gcggctgcgg atggtgaccg cgatgacgat
      661 gacgattgat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag
      721 atactgagca ctactagaga aagaggagaa atactagagc caggcatcaa ataaaacgaa
      781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//