LOCUS       BBa_K352015             1032 bp    DNA     linear       24-Jan-2026
DEFINITION  CooA coupled with placI+RBS+Double Terminator
ACCESSION   BBa_K352015
VERSION     BBa_K352015.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start Codon
     CDS             227..895
                     /label=BBa_K352001
     stem_loop       915..958
                     /label=stem_loop
     misc_feature    904..983
                     /label=BBa_B0010
     terminator      904..983
                     /label=BBa_B0010
     stem_loop       999..1018
                     /label=T7 TE
     misc_feature    1025..1025
                     /label=stop
     polyA_site      1019..1032
                     /label=polya
     misc_feature    992..1032
                     /label=BBa_B0012
     terminator      992..1032
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc caccacgctt
      241 caatattgca aacgtactgc tgtctcctga tggtgagacg ttcttccgcg gttttcgctc
      301 taagattcat gcgaaaggct ctctggtatg tactggtgaa ggtgatgaaa acggtgtttt
      361 tgttgtggtt gatggtcgcc tgcgtgttta cctggttggt gaggagcgtg agattagcct
      421 gttctacctg acttccggcg atatgttctg catgcattcc ggctgcctgg ttgaagccac
      481 cgagcgcacc gaagtgcgtt tcgccgatat ccgcacgttc gagcagaaac tgcaaacctg
      541 tccgtctatg gcatggggcc tgatcgccat tctgggccgt gctctgacct cctgtatgcg
      601 taccatcgaa gacctgatgt tccacgatat taaacaacgt atcgcgggct ttttcatcga
      661 ccacgctaac actaccggtc gccagactca gggtggcgta attgtttctg ttgacttcac
      721 tgtagaggaa atcgctaatc tgatcggtag ctcccgccag actactagca cggcgctgaa
      781 ctctctgatt aaagagggtt acatctcccg ccagggccgt ggtcactata ctatcccgaa
      841 cctggttcgc ctgaaggcgg ctgcggatgg tgaccgcgat gacgatgacg attgatacta
      901 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      961 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1021 tctgcgttta ta
//