LOCUS       BBa_K352016             1923 bp    DNA     linear       26-Nov-2024
DEFINITION  pCooF RBS GFP Double Terminator pCooM RBS RFP Double Terminator
ACCESSION   BBa_K352016
VERSION     BBa_K352016.1  
FEATURES             Location/Qualifiers
     misc_feature    8..33
                     /label=A+T rich sequence
     misc_feature    41..44
                     /label=CooA binding site
     misc_feature    52..56
                     /label=CooA binding site
     misc_feature    79..84
                     /label=-10 Region
     misc_feature    92..93
                     /label=Transcription Start
     misc_feature    36..61
                     /label=Protected by CooA
     misc_feature    23..91
                     /label=Protected by CooA+RNAP
     promoter        1..94
                     /label=BBa_K352002
     RBS             103..117
                     /label=RBS-1\Strong
     RBS             110..114
                     /label=RBS
     misc_feature    103..117
                     /label=BBa_B0030
     RBS             103..117
                     /label=BBa_B0030
     CDS             124..843
                     /label=GFP protein
     CDS             124..843
                     /label=BBa_E0040
     stem_loop       863..906
                     /label=stem_loop
     misc_feature    852..931
                     /label=BBa_B0010
     terminator      852..931
                     /label=BBa_B0010
     stem_loop       947..966
                     /label=T7 TE
     misc_feature    973..973
                     /label=stop
     polyA_site      967..980
                     /label=polya
     misc_feature    940..980
                     /label=BBa_B0012
     terminator      940..980
                     /label=BBa_B0012
     misc_feature    103..980
                     /label=BBa_E0840
     misc_feature    1..980
                     /label=BBa_K352010
     misc_feature    1002..1006
                     /label=CooA binding Site
     misc_feature    1013..1017
                     /label=CooA binding Site
     promoter        989..1054
                     /label=BBa_K352003
     misc_feature    1067..1070
                     /label=conserved
     RBS             1063..1074
                     /label=BBa_B0034
     CDS             1081..1755
                     /label=mrfp1
     misc_feature    1762..1786
                     /label=Help:Barcodes
     CDS             1081..1761
                     /label=BBa_E1010
     stem_loop       1806..1849
                     /label=stem_loop
     misc_feature    1795..1874
                     /label=BBa_B0010
     terminator      1795..1874
                     /label=BBa_B0010
     stem_loop       1890..1909
                     /label=T7 TE
     misc_feature    1916..1916
                     /label=stop
     polyA_site      1910..1923
                     /label=polya
     misc_feature    1883..1923
                     /label=BBa_B0012
     terminator      1883..1923
                     /label=BBa_B0012
     misc_feature    1063..1923
                     /label=BBa_I13507
     misc_feature    989..1923
                     /label=BBa_K352011
ORIGIN
        1 ttcggcgtct tttcataccc ccataaaaac tctggataac tgtcatctgg ccgacagacg
       61 gggccgggct ttttgtcgct tactcggcgc caggtactag agattaaaga ggagaaatac
      121 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      181 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      241 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      301 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      361 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      421 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      481 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      541 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      601 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      661 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      721 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      781 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
      841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      961 tgggcctttc tgcgtttata tactagagac agcatgacga gtgtcggcca agtgacggaa
     1021 ttttccgccc aggtggcgta ctcccgttct cacttactag agaaagagga gaaatactag
     1081 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
     1141 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
     1201 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
     1261 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
     1321 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
     1381 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
     1441 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
     1501 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
     1561 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
     1621 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
     1681 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
     1741 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
     1801 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1861 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1921 ata
//