LOCUS       BBa_K352017             1838 bp    DNA     linear       17-May-2024
DEFINITION  pCooM RBS RFP Double terminator CooA coupled with placI+RBS
ACCESSION   BBa_K352017
VERSION     BBa_K352017.1  
FEATURES             Location/Qualifiers
     misc_feature    14..18
                     /label=CooA binding Site
     misc_feature    25..29
                     /label=CooA binding Site
     promoter        1..66
                     /label=BBa_K352003
     misc_feature    79..82
                     /label=conserved
     RBS             75..86
                     /label=BBa_B0034
     CDS             93..767
                     /label=mrfp1
     misc_feature    774..798
                     /label=Help:Barcodes
     CDS             93..773
                     /label=BBa_E1010
     stem_loop       818..861
                     /label=stem_loop
     misc_feature    807..886
                     /label=BBa_B0010
     terminator      807..886
                     /label=BBa_B0010
     stem_loop       902..921
                     /label=T7 TE
     misc_feature    928..928
                     /label=stop
     polyA_site      922..935
                     /label=polya
     misc_feature    895..935
                     /label=BBa_B0012
     terminator      895..935
                     /label=BBa_B0012
     misc_feature    75..935
                     /label=BBa_I13507
     misc_feature    1..935
                     /label=BBa_K352011
     CDS             944..1031
                     /label=end of LacI coding region (inactive)
     misc_feature    944..1143
                     /label=BBa_R0010
     misc_feature    1032..1069
                     /label=CAP binding site
     promoter        1080..1085
                     /label=-35
     promoter        1104..1109
                     /label=-10
     misc_feature    1109..1143
                     /label=LacI binding site
     misc_feature    1116..1116
                     /label=start
     promoter        944..1143
                     /label=BBa_R0010
     misc_feature    1156..1159
                     /label=conserved
     RBS             1152..1163
                     /label=BBa_B0034
     misc_feature    1170..1172
                     /label=Start Codon
     CDS             1170..1838
                     /label=BBa_K352001
     misc_feature    944..1838
                     /label=BBa_K352014
ORIGIN
        1 acagcatgac gagtgtcggc caagtgacgg aattttccgc ccaggtggcg tactcccgtt
       61 ctcacttact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag
      121 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
      181 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
      241 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
      301 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
      361 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
      421 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
      481 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
      541 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
      601 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
      661 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
      721 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat
      781 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      901 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg caaaccgcct
      961 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1021 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1081 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1141 acatactaga gaaagaggag aaatactaga tgccaccacg cttcaatatt gcaaacgtac
     1201 tgctgtctcc tgatggtgag acgttcttcc gcggttttcg ctctaagatt catgcgaaag
     1261 gctctctggt atgtactggt gaaggtgatg aaaacggtgt ttttgttgtg gttgatggtc
     1321 gcctgcgtgt ttacctggtt ggtgaggagc gtgagattag cctgttctac ctgacttccg
     1381 gcgatatgtt ctgcatgcat tccggctgcc tggttgaagc caccgagcgc accgaagtgc
     1441 gtttcgccga tatccgcacg ttcgagcaga aactgcaaac ctgtccgtct atggcatggg
     1501 gcctgatcgc cattctgggc cgtgctctga cctcctgtat gcgtaccatc gaagacctga
     1561 tgttccacga tattaaacaa cgtatcgcgg gctttttcat cgaccacgct aacactaccg
     1621 gtcgccagac tcagggtggc gtaattgttt ctgttgactt cactgtagag gaaatcgcta
     1681 atctgatcgg tagctcccgc cagactacta gcacggcgct gaactctctg attaaagagg
     1741 gttacatctc ccgccagggc cgtggtcact atactatccc gaacctggtt cgcctgaagg
     1801 cggctgcgga tggtgaccgc gatgacgatg acgattga
//