##gff-version 3
##sequence-region BBa_K352017 1 1838
BBa_K352017	.	engineered_region	944	1838	.	+	0	ID=BBa_K352014;Name=BBa_K352014
BBa_K352017	.	ribosome_entry_site	1152	1163	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K352014
BBa_K352017	.	conserved_region	1156	1159	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K352017	.	promoter	944	1143	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K352014
BBa_K352017	.	non_covalent_binding_site	1109	1143	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K352017	.	promoter	1080	1085	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K352017	.	engineered_region	944	1143	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K352017	.	start_codon	1116	1116	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K352017	.	non_covalent_binding_site	1032	1069	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K352017	.	promoter	1104	1109	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K352017	.	CDS	944	1031	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K352017	.	CDS	1170	1838	.	+	0	ID=BBa_K352001;Name=BBa_K352001;Parent=BBa_K352014
BBa_K352017	.	start_codon	1170	1172	.	+	0	ID=annotation2081251;Name=Start Codon;Parent=BBa_K352001
BBa_K352017	.	engineered_region	1	935	.	+	0	ID=BBa_K352011;Name=BBa_K352011
BBa_K352017	.	engineered_region	75	935	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K352011
BBa_K352017	.	terminator	895	935	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K352017	.	stem_loop	902	921	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K352017	.	engineered_region	895	935	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K352017	.	stop_codon	928	928	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K352017	.	polyA_site	922	935	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K352017	.	ribosome_entry_site	75	86	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K352017	.	conserved_region	79	82	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K352017	.	terminator	807	886	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K352017	.	stem_loop	818	861	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K352017	.	engineered_region	807	886	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K352017	.	CDS	93	773	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K352017	.	CDS	93	767	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K352017	.	engineered_tag	774	798	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K352017	.	promoter	1	66	.	+	0	ID=BBa_K352003;Name=BBa_K352003;Parent=BBa_K352011
BBa_K352017	.	non_covalent_binding_site	14	18	.	+	0	ID=annotation2081259;Name=CooA binding Site;Parent=BBa_K352003
BBa_K352017	.	non_covalent_binding_site	25	29	.	+	0	ID=annotation2081260;Name=CooA binding Site;Parent=BBa_K352003
>BBa_K352017
acagcatgacgagtgtcggccaagtgacggaattttccgcccaggtggcgtactcccgttctcacttactagagaaaga
ggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgtt
aacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtta
ccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaaca
cccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagac
ggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacca
acttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaaga
cggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacc
tacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaag
actacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtg
tagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
gtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat
gttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgccaccacgcttca
atattgcaaacgtactgctgtctcctgatggtgagacgttcttccgcggttttcgctctaagattcatgcgaaaggctc
tctggtatgtactggtgaaggtgatgaaaacggtgtttttgttgtggttgatggtcgcctgcgtgtttacctggttggt
gaggagcgtgagattagcctgttctacctgacttccggcgatatgttctgcatgcattccggctgcctggttgaagcca
ccgagcgcaccgaagtgcgtttcgccgatatccgcacgttcgagcagaaactgcaaacctgtccgtctatggcatgggg
cctgatcgccattctgggccgtgctctgacctcctgtatgcgtaccatcgaagacctgatgttccacgatattaaacaa
cgtatcgcgggctttttcatcgaccacgctaacactaccggtcgccagactcagggtggcgtaattgtttctgttgact
tcactgtagaggaaatcgctaatctgatcggtagctcccgccagactactagcacggcgctgaactctctgattaaaga
gggttacatctcccgccagggccgtggtcactatactatcccgaacctggttcgcctgaaggcggctgcggatggtgac
cgcgatgacgatgacgattga