BBa_K354000
1
BBa_K354000
Lac/Ara-1 IPTG Inducible Promoter
2010-10-18T11:00:00Z
2015-05-08T01:12:11Z
The promoter was synthesised via primer extension.
Derived from the L-arabinose and Lactose operons, this synthetic promoter remains in the 'off' state due to the repression of the AraC protein. AraC is constitutively expressed in bacteria possessing the arabinose operon and as such is present in E. coli. When exogenous arabinose and/or IPTG is added, the AraC protein is freed from the promoter thus allowing RNA polymerase to bind and initiate transcription. Hence, addition of arabinose or IPTG will switch the promoter into the 'on' state.
false
false
_481_
0
6469
9
It's complicated
false
The affiliation between AraC and its binding sequence had to be ensured so as to prevent basal expression and false triggering of the promoter.
false
Gregory Meyer
annotation2090005
1
Lac Operon operator Sequence
range2090005
1
72
102
annotation2090003
1
Symmetrical Synthetic Operator
range2090003
1
44
57
annotation2090001
1
-33 Hexamer
range2090001
1
38
43
annotation2090002
1
AraC Binding Site
range2090002
1
2
37
annotation2090004
1
-10 Hexamer
range2090004
1
58
66
BBa_K354000_sequence
1
gcatagcatttttatccataagattagcggatctaacctttacaattgtgagcgctcacaattatgatagattcaattgtgagcggataacaatttcacacagat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z