BBa_K354003
1
BBa_K354003
PlcR Promoter
2010-10-19T11:00:00Z
2015-05-08T01:12:11Z
The sequence is derived from the PlcR promoter in Bacillus thuringiensis and was synthesized via primer extension.
The PlcR promoter is positively regulated by the PlcR-PapR fusion peptide and is repressed by the SpooA peptide. Derived from Bacillus thuringiensis, the PlcR promoter regulates the expression of a host of genes, one of which is the PlcR gene itself. Thus there exists a positive-feedback loop controlling the activation of the operon. Within the promoter are clearly defined binding regions for both the PlcR-PapR fusion peptide as well as the SpooA repressor protein.
false
false
_481_
0
6469
9
It's complicated
false
So as to create a library of promoters with differing transcriptional activities, randomised nucleotides were introduced into the -10 box - the binding site for the RNAP sigma-factor.
false
Gregory Meyer
annotation2090418
1
PlcR binding site
range2090418
1
27
43
annotation2093188
1
SpoOA binding site
range2093188
1
51
57
annotation2093187
1
SpoOA binding site
range2093187
1
9
15
annotation2090417
1
Sigma A binding site
range2090417
1
64
69
annotation2090419
1
Transcriptional start site
range2090419
1
75
75
BBa_K354003_sequence
1
gaaaaatttgccgaattttatatatatatgcattatttcatatcaaaaattgtcgaatccacattattgtagtggtatgacaactt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z