BBa_K356000
1
CqsA
CAI-1 autoinducer synthase
2010-08-31T11:00:00Z
2015-05-08T01:12:11Z
Vibrio cholerae genomic sequence, from NCBI database, synthesized by GeneArt.
CqsA gene from Vibrio cholerae, codes for the CAI-1 autoinducer synthase. This autoinducer is specific to V. cholerae and is detected by CqsS.
false
false
_467_
0
7465
9
It's complicated
true
Codon optimized for expression in E. coli.
false
C David Owen, James Taylor
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K356001
1
CqsA
CAI-1 Generator
2010-08-31T11:00:00Z
2015-05-08T01:12:11Z
The CqsA coding reigon (K356000) comes from V. cholerae genomic sequence (NCBI database) synthesized by GeneArt. The rest of the subparts are from the registry.
Vibrio cholerae CqsA with strong constituitive promotor, ribosome binding site and terminator. Generates CAI-1, a V. cholerae specific autoinducer which is detected by CqsS.
false
false
_467_
0
7465
9
It's complicated
true
CqsA codon optimized by Mr Gene. Biobrick scars were placed between all parts as would be produced by standard assembly.
false
C David Owen, James Taylor
component2222532
1
BBa_K356000
component2222539
1
BBa_B0015
component2222530
1
BBa_B0034
component2222531
1
BBa_J23100
annotation2222530
1
BBa_B0034
range2222530
1
1
12
annotation2222532
1
BBa_K356000
range2222532
1
64
1486
annotation2222531
1
BBa_J23100
range2222531
1
21
55
annotation2222539
1
BBa_B0015
range2222539
1
1495
1623
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K356001_sequence
1
aaagaggagaaatactagagttgacggctagctcagtcctaggtacagtgctagctactagaggcgcgccgaattcgcggccgcttctagagttgacggctagctcagtcctaggtacagtgctagctactagaaagaggagaaatactagatgaacaaacctcagctgcctgactttatccaaaacaaaatcgaccactatatcgagaactatttcgacattaacaaaaacggcaaacacctggtgctgggcaaacaagcatcaccggatgacattatcctgcaaagcaacgactatctggccctggctaatcaccctctgatcaaagctcgtctggcgaaaagcctgctggaagaacagcaatccctgtttatgagcgcctcctttctgcaaaacgattatgacaaaccgatgattgagaaacgcctggccaaattcactggtttcgatgaatgcctgctgtctcagtctggttggaatgccaatgttggtctgctgcaaacaatctgtcagcctaacacgaacgtctatattgatttcttcgcccacatgtcgctgtgggaaggtgctcgttatgctaatgctcaggcccatccgtttatgcacaacaactgtgaccatctgcgtatgctgattcagcgtcacggtcctggtattatcgtcgtggactccatctattctaccctggggaccattgctccactggctgaactggtgaatatcagtaaagagtttgggtgtgccctgctggttgatgaaagccattctctgggaacccatggcccgaacggtgccgggctgctggcggagctgggtctgacacgtgaagttcacttcatgaccgcttcgctggcaaaaacattcgcctatcgtgctggtgccatttggtgtaacaacgaggttaatcgctgtgttccgttcatctcttatccggccatctttagcagtacactgctgccgtatgaagctgctggtctggaaacaaccctggagattatcgagtctgccgataaccgtcgtcaacatctggatcgtatggcccgtaaactgcgtattggtctgtcccaactgggtctgacaattcgtagcgaatctcagattattggcctggagactggtgacgagcgtaataccgagaaagtccgtgattatctggagtctaacggcgtgtttggtagcgttttttgtcgtccggcaacctctaaaaacaaaaacatcatccgcctgtccctgaatagtgatgtgaatgatgagcagatcgctaaaatcattgaagtctgttcggatgccgtgaattatggtgacttctatttccgctgatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagttaattaactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K356000_sequence
1
gcgcgccgaattcgcggccgcttctagagttgacggctagctcagtcctaggtacagtgctagctactagaaagaggagaaatactagatgaacaaacctcagctgcctgactttatccaaaacaaaatcgaccactatatcgagaactatttcgacattaacaaaaacggcaaacacctggtgctgggcaaacaagcatcaccggatgacattatcctgcaaagcaacgactatctggccctggctaatcaccctctgatcaaagctcgtctggcgaaaagcctgctggaagaacagcaatccctgtttatgagcgcctcctttctgcaaaacgattatgacaaaccgatgattgagaaacgcctggccaaattcactggtttcgatgaatgcctgctgtctcagtctggttggaatgccaatgttggtctgctgcaaacaatctgtcagcctaacacgaacgtctatattgatttcttcgcccacatgtcgctgtgggaaggtgctcgttatgctaatgctcaggcccatccgtttatgcacaacaactgtgaccatctgcgtatgctgattcagcgtcacggtcctggtattatcgtcgtggactccatctattctaccctggggaccattgctccactggctgaactggtgaatatcagtaaagagtttgggtgtgccctgctggttgatgaaagccattctctgggaacccatggcccgaacggtgccgggctgctggcggagctgggtctgacacgtgaagttcacttcatgaccgcttcgctggcaaaaacattcgcctatcgtgctggtgccatttggtgtaacaacgaggttaatcgctgtgttccgttcatctcttatccggccatctttagcagtacactgctgccgtatgaagctgctggtctggaaacaaccctggagattatcgagtctgccgataaccgtcgtcaacatctggatcgtatggcccgtaaactgcgtattggtctgtcccaactgggtctgacaattcgtagcgaatctcagattattggcctggagactggtgacgagcgtaataccgagaaagtccgtgattatctggagtctaacggcgtgtttggtagcgttttttgtcgtccggcaacctctaaaaacaaaaacatcatccgcctgtccctgaatagtgatgtgaatgatgagcagatcgctaaaatcattgaagtctgttcggatgccgtgaattatggtgacttctatttccgctgatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagttaattaac
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z