BBa_K356000 1 CqsA CAI-1 autoinducer synthase 2010-08-31T11:00:00Z 2015-05-08T01:12:11Z Vibrio cholerae genomic sequence, from NCBI database, synthesized by GeneArt. CqsA gene from Vibrio cholerae, codes for the CAI-1 autoinducer synthase. This autoinducer is specific to V. cholerae and is detected by CqsS. false false _467_ 0 7465 9 It's complicated true Codon optimized for expression in E. coli. false C David Owen, James Taylor BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K356001 1 CqsA CAI-1 Generator 2010-08-31T11:00:00Z 2015-05-08T01:12:11Z The CqsA coding reigon (K356000) comes from V. cholerae genomic sequence (NCBI database) synthesized by GeneArt. The rest of the subparts are from the registry. Vibrio cholerae CqsA with strong constituitive promotor, ribosome binding site and terminator. Generates CAI-1, a V. cholerae specific autoinducer which is detected by CqsS. false false _467_ 0 7465 9 It's complicated true CqsA codon optimized by Mr Gene. Biobrick scars were placed between all parts as would be produced by standard assembly. false C David Owen, James Taylor component2222532 1 BBa_K356000 component2222539 1 BBa_B0015 component2222530 1 BBa_B0034 component2222531 1 BBa_J23100 annotation2222530 1 BBa_B0034 range2222530 1 1 12 annotation2222532 1 BBa_K356000 range2222532 1 64 1486 annotation2222531 1 BBa_J23100 range2222531 1 21 55 annotation2222539 1 BBa_B0015 range2222539 1 1495 1623 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K356001_sequence 1 aaagaggagaaatactagagttgacggctagctcagtcctaggtacagtgctagctactagaggcgcgccgaattcgcggccgcttctagagttgacggctagctcagtcctaggtacagtgctagctactagaaagaggagaaatactagatgaacaaacctcagctgcctgactttatccaaaacaaaatcgaccactatatcgagaactatttcgacattaacaaaaacggcaaacacctggtgctgggcaaacaagcatcaccggatgacattatcctgcaaagcaacgactatctggccctggctaatcaccctctgatcaaagctcgtctggcgaaaagcctgctggaagaacagcaatccctgtttatgagcgcctcctttctgcaaaacgattatgacaaaccgatgattgagaaacgcctggccaaattcactggtttcgatgaatgcctgctgtctcagtctggttggaatgccaatgttggtctgctgcaaacaatctgtcagcctaacacgaacgtctatattgatttcttcgcccacatgtcgctgtgggaaggtgctcgttatgctaatgctcaggcccatccgtttatgcacaacaactgtgaccatctgcgtatgctgattcagcgtcacggtcctggtattatcgtcgtggactccatctattctaccctggggaccattgctccactggctgaactggtgaatatcagtaaagagtttgggtgtgccctgctggttgatgaaagccattctctgggaacccatggcccgaacggtgccgggctgctggcggagctgggtctgacacgtgaagttcacttcatgaccgcttcgctggcaaaaacattcgcctatcgtgctggtgccatttggtgtaacaacgaggttaatcgctgtgttccgttcatctcttatccggccatctttagcagtacactgctgccgtatgaagctgctggtctggaaacaaccctggagattatcgagtctgccgataaccgtcgtcaacatctggatcgtatggcccgtaaactgcgtattggtctgtcccaactgggtctgacaattcgtagcgaatctcagattattggcctggagactggtgacgagcgtaataccgagaaagtccgtgattatctggagtctaacggcgtgtttggtagcgttttttgtcgtccggcaacctctaaaaacaaaaacatcatccgcctgtccctgaatagtgatgtgaatgatgagcagatcgctaaaatcattgaagtctgttcggatgccgtgaattatggtgacttctatttccgctgatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagttaattaactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K356000_sequence 1 gcgcgccgaattcgcggccgcttctagagttgacggctagctcagtcctaggtacagtgctagctactagaaagaggagaaatactagatgaacaaacctcagctgcctgactttatccaaaacaaaatcgaccactatatcgagaactatttcgacattaacaaaaacggcaaacacctggtgctgggcaaacaagcatcaccggatgacattatcctgcaaagcaacgactatctggccctggctaatcaccctctgatcaaagctcgtctggcgaaaagcctgctggaagaacagcaatccctgtttatgagcgcctcctttctgcaaaacgattatgacaaaccgatgattgagaaacgcctggccaaattcactggtttcgatgaatgcctgctgtctcagtctggttggaatgccaatgttggtctgctgcaaacaatctgtcagcctaacacgaacgtctatattgatttcttcgcccacatgtcgctgtgggaaggtgctcgttatgctaatgctcaggcccatccgtttatgcacaacaactgtgaccatctgcgtatgctgattcagcgtcacggtcctggtattatcgtcgtggactccatctattctaccctggggaccattgctccactggctgaactggtgaatatcagtaaagagtttgggtgtgccctgctggttgatgaaagccattctctgggaacccatggcccgaacggtgccgggctgctggcggagctgggtctgacacgtgaagttcacttcatgaccgcttcgctggcaaaaacattcgcctatcgtgctggtgccatttggtgtaacaacgaggttaatcgctgtgttccgttcatctcttatccggccatctttagcagtacactgctgccgtatgaagctgctggtctggaaacaaccctggagattatcgagtctgccgataaccgtcgtcaacatctggatcgtatggcccgtaaactgcgtattggtctgtcccaactgggtctgacaattcgtagcgaatctcagattattggcctggagactggtgacgagcgtaataccgagaaagtccgtgattatctggagtctaacggcgtgtttggtagcgttttttgtcgtccggcaacctctaaaaacaaaaacatcatccgcctgtccctgaatagtgatgtgaatgatgagcagatcgctaaaatcattgaagtctgttcggatgccgtgaattatggtgacttctatttccgctgatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagttaattaac BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z