LOCUS       BBa_K356100             2575 bp    DNA     linear       01-Jun-2024
DEFINITION  Lux Quorum Sensing Bistable Expression
ACCESSION   BBa_K356100
VERSION     BBa_K356100.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    160..179
                     /label=LuxR/HSL
     promoter        179..184
                     /label=-35
     promoter        201..206
                     /label=-10
     misc_feature    212..212
                     /label=start
     misc_feature    160..214
                     /label=BBa_R0062
     promoter        160..214
                     /label=BBa_R0062
     RBS             223..235
                     /label=RBS-3\Weak
     RBS             229..232
                     /label=RBS
     misc_feature    223..235
                     /label=BBa_B0032
     RBS             223..235
                     /label=BBa_B0032
     misc_feature    242..243
                     /label=prefix
     misc_feature    415..415
                     /label=T
     misc_feature    733..733
                     /label=A
     CDS             242..991
                     /label=luxR
     misc_feature    242..997
                     /label=BBa_C0062
     misc_feature    998..1022
                     /label=Help:Barcodes
     CDS             242..997
                     /label=BBa_C0062
     misc_feature    1035..1038
                     /label=conserved
     RBS             1031..1042
                     /label=BBa_B0034
     misc_feature    1628..1659
                     /label=LVA
     CDS             1049..1627
                     /label=luxI
     misc_feature    1049..1666
                     /label=BBa_C0061
     misc_feature    1667..1691
                     /label=Help:Barcodes
     CDS             1049..1666
                     /label=BBa_C0061
     RBS             1700..1712
                     /label=RBS-3\Weak
     RBS             1706..1709
                     /label=RBS
     misc_feature    1700..1712
                     /label=BBa_B0032
     RBS             1700..1712
                     /label=BBa_B0032
     CDS             1719..2438
                     /label=GFP protein
     CDS             1719..2438
                     /label=BBa_E0040
     stem_loop       2458..2501
                     /label=stem_loop
     misc_feature    2447..2526
                     /label=BBa_B0010
     terminator      2447..2526
                     /label=BBa_B0010
     stem_loop       2542..2561
                     /label=T7 TE
     misc_feature    2568..2568
                     /label=stop
     polyA_site      2562..2575
                     /label=polya
     misc_feature    2535..2575
                     /label=BBa_B0012
     terminator      2535..2575
                     /label=BBa_B0012
     misc_feature    1700..2575
                     /label=BBa_E0240
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga cctgtaggat cgtacaggtt
      181 tacgcaagaa aatggtttgt tatagtcgaa taaatactag agtcacacag gaaagtacta
      241 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      301 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      361 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      421 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      481 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      541 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      601 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      661 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      721 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      781 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      841 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      901 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      961 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
     1021 actactagag aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt
     1081 ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca
     1141 aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc
     1201 aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt
     1261 attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca
     1321 gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag
     1381 ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa
     1441 acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg
     1501 atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt
     1561 aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt
     1621 cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta
     1681 gtgtagatct ctactagagt cacacaggaa agtactagat gcgtaaagga gaagaacttt
     1741 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     1801 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     1861 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
     1921 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
     1981 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     2041 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     2101 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     2161 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     2221 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     2281 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     2341 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     2401 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
     2461 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2521 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//