##gff-version 3
##sequence-region BBa_K356100 1 2575
BBa_K356100	.	CDS	242	997	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K356100	.	engineered_region	242	243	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K356100	.	engineered_tag	998	1022	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K356100	.	point_mutation	733	733	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K356100	.	engineered_region	242	997	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K356100	.	point_mutation	415	415	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K356100	.	CDS	242	991	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K356100	.	ribosome_entry_site	1031	1042	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K356100	.	conserved_region	1035	1038	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K356100	.	ribosome_entry_site	223	235	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K356100	.	ribosome_entry_site	229	232	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K356100	.	ribosome_entry_site	223	235	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K356100	.	engineered_region	223	235	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K356100	.	promoter	160	214	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K356100	.	non_covalent_binding_site	160	179	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K356100	.	promoter	179	184	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K356100	.	promoter	201	206	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K356100	.	engineered_region	160	214	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K356100	.	start_codon	212	212	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K356100	.	CDS	1049	1666	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K356100	.	engineered_region	1049	1666	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K356100	.	tag	1628	1659	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K356100	.	CDS	1049	1627	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K356100	.	engineered_tag	1667	1691	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K356100	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_K356100	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K356100	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K356100	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K356100	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K356100	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K356100	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K356100	.	engineered_region	1700	2575	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K356100	.	terminator	2447	2526	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K356100	.	stem_loop	2458	2501	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K356100	.	engineered_region	2447	2526	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K356100	.	terminator	2535	2575	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K356100	.	stem_loop	2542	2561	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K356100	.	stop_codon	2568	2568	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K356100	.	polyA_site	2562	2575	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K356100	.	engineered_region	2535	2575	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K356100	.	ribosome_entry_site	1700	1712	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K356100	.	ribosome_entry_site	1706	1709	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K356100	.	ribosome_entry_site	1700	1712	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K356100	.	engineered_region	1700	1712	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K356100	.	CDS	1719	2438	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K356100	.	CDS	1719	2438	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K356100
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagta
ctagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatatt
aatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatgg
ttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaata
tgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaa
aaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaaca
atggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaa
cataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgattta
accaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgca
gtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaa
agcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactacta
gagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaag
gtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcaga
tgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttatta
cctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaata
tagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaact
atttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcga
tttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttg
tattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctctactagagtcacacaggaaagtactagatgcgtaaaggagaagaact
tttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggt
gaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacac
ttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagag
tgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtc
aagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggac
acaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaactt
caaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggc
cctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccaca
tggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata