BBa_K357001 1 BBa_K357001 muconate and chloromuconate cycloisomerase 2010-06-22T11:00:00Z 2015-05-08T01:12:11Z AUTHORS Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D., Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L., Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R., Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P., Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T., Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J., Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K., Duesterhoft,A., Tummler,B. and Fraser,C. TITLE Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440 JOURNAL Environ. Microbiol. 4 (12), 799-808 (2002) Muconate Lactonizing Enzyme (MLE), an homooctameric enzyme, catalyses the conversion of cis,cis-muconate (CCM) to muconolactone (ML) in the catechol branch of the beta-ketoadipate pathway. This pathway is used in soil microbes to breakdown lignin-derived aromatics, catechol and protocatechuate, to citric acid cycle intermediates. Some bacterial species are also capable of dehalogenating chloroaromatic compounds by the action of chloromuconate lactonizing enzymes (Cl-MLEs). MLEs are members of the enolase superfamily characterized by the presence of an enolate anion intermediate which is generated by abstraction of the alpha-proton of the carboxylate substrate by an active site residue and that is stabilized by coordination to the essential Mg2+ ion. false false _530_ 0 5655 9 Not in stock true The sequence was changed from the original to remove a PstI restriction site located around bp 124, to remove the restriction site while maintaining a conserved aa sequence. false Anish Kapadia annotation2072353 1 Stop Codon range2072353 1 1120 1122 annotation2072352 1 Start Codon range2072352 1 1 3 BBa_K357001_sequence 1 atgacctccgtgctgatcgaacacattgatgccattatcgtggacctgccgactatccgccctcacaaactggccatgcatacgatgcaacagcagaccctggttgttctgcgcctgcgctgtagtgatggtgttgaaggcattggtgaagccaccacaattggaggactggcctatggttatgaaagcccggaagggatcaaagccaatattgacgcctatctggcaccagctctgattgggctgccagctgacaacattaacgctgctatgctgaaactggataaactggcgaaaggcaacacatttgccaaaagcggcattgaatctgccctgctggatgctcagggtaaacgtctgggactgcctgtgtctgaactgctgggtggtcgtgtccgtgattccctggaagttgcttggactctggctagtggcgatactgctcgtgacattgccgaagcacagcacatgctggacattcgtcgtcaccgtgtctttaaactgaaaatcggcgccaacccggttgctcaagacctgaaacacgtggtagcgattaaacgtgaactgggtgattctgcctccgttcgtgtggatgtgaaccagtattgggatgagagtcaggcaattcgtgcctgccaagttctgggggataacgggattgacctgatcgaacagcctattagccgtattaaccgtgccggtcaggttcgtctgaatcagcgtagtcctgctccgattatggccgatgagtccatcgaaagcgtggaagatgcttttagcctggcggctgatggtgctgctagcatttttgctctgaaaattgccaaaaacggtggtcctcgtgccgtactgcgtacagctcaaatcgccgaggctgctggtattgccctgtatggtgggacaatgctggaaggctctattggtacactggcaagcgctcatgcttttctgacactgcgccagctgacatggggtactgaactgtttggaccgctgctgctgacagaagaaatcgtcaacgagcctccacaatatcgtgatttccaactgcatatccctcatactccaggcctgggactgacactggatgaacagcgtctggctcgctttgctcgtcgttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z