LOCUS       BBa_K358000              939 bp    DNA     linear       29-Nov-2024
DEFINITION  Lac promoter with GFP
ACCESSION   BBa_K358000
VERSION     BBa_K358000.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..802
                     /label=GFP protein
     CDS             83..802
                     /label=BBa_E0040
     stem_loop       822..865
                     /label=stem_loop
     misc_feature    811..890
                     /label=BBa_B0010
     terminator      811..890
                     /label=BBa_B0010
     stem_loop       906..925
                     /label=T7 TE
     misc_feature    932..932
                     /label=stop
     polyA_site      926..939
                     /label=polya
     misc_feature    899..939
                     /label=BBa_B0012
     terminator      899..939
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      781 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      901 acactggctc accttcgggt gggcctttct gcgtttata
//