BBa_K358005
1
BBa_K358005
lambda lysis cassette [SRRz]
2010-09-16T11:00:00Z
2015-05-08T01:12:12Z
λ -Hind Ⅲ digest(product of TaKaRa Bio), BBa_B0015
C. Chang, K. Nam, and R. Young, ???S gene expression and the timing of lysis by bacteriophage lambda,??? J. Bacteriol., vol. 177, Jun. 1995, pp. 3283-3294.
This part is to lead cell lysis. It contains S[holin/anti-holin], R[endolysin] and Rz gene with wt-RBS and double terminator[BBa_B0015].
true
false
_455_
0
7170
9
Discontinued
false
We took this gene by PCR from λ -Hind Ⅲ digest, which is the product of TaKaRa Bio. However, there is a mutation on S gene and it's not the wild-type. So, after, we done the point mutation PCR and prepared the wild-type DNA.
false
Ken Kajita
annotation2080434
1
antiholin
range2080434
1
29
352
annotation2080437
1
Rz
range2080437
1
809
1270
annotation2080433
1
wt-RBS
range2080433
1
2
26
annotation2080436
1
endolysin
range2080436
1
336
812
annotation2080445
1
stop
range2080445
1
1677
1677
annotation2080441
1
polya
range2080441
1
1534
1547
annotation2080443
1
stem_loop
range2080443
1
1567
1610
annotation2080442
1
stop
range2080442
1
1540
1540
annotation2080444
1
T7 TE
range2080444
1
1651
1670
annotation2080438
1
Rz1
range2080438
1
1029
1211
annotation2080435
1
holin
range2080435
1
35
352
annotation2080440
1
B0012
range2080440
1
1370
1410
annotation2080439
1
B0010
range2080439
1
1282
1361
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z