BBa_K358005 1 BBa_K358005 lambda lysis cassette [SRRz] 2010-09-16T11:00:00Z 2015-05-08T01:12:12Z λ -Hind Ⅲ digest(product of TaKaRa Bio), BBa_B0015 C. Chang, K. Nam, and R. Young, ???S gene expression and the timing of lysis by bacteriophage lambda,??? J. Bacteriol., vol. 177, Jun. 1995, pp. 3283-3294. This part is to lead cell lysis. It contains S[holin/anti-holin], R[endolysin] and Rz gene with wt-RBS and double terminator[BBa_B0015]. true false _455_ 0 7170 9 Discontinued false We took this gene by PCR from λ -Hind Ⅲ digest, which is the product of TaKaRa Bio. However, there is a mutation on S gene and it's not the wild-type. So, after, we done the point mutation PCR and prepared the wild-type DNA. false Ken Kajita annotation2080435 1 holin range2080435 1 35 352 annotation2080438 1 Rz1 range2080438 1 1029 1211 annotation2080445 1 stop range2080445 1 1677 1677 annotation2080434 1 antiholin range2080434 1 29 352 annotation2080433 1 wt-RBS range2080433 1 2 26 annotation2080437 1 Rz range2080437 1 809 1270 annotation2080440 1 B0012 range2080440 1 1370 1410 annotation2080436 1 endolysin range2080436 1 336 812 annotation2080444 1 T7 TE range2080444 1 1651 1670 annotation2080439 1 B0010 range2080439 1 1282 1361 annotation2080443 1 stem_loop range2080443 1 1567 1610 annotation2080441 1 polya range2080441 1 1534 1547 annotation2080442 1 stop range2080442 1 1540 1540 igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z