LOCUS       BBa_K358006             1410 bp    DNA     linear       30-Apr-2024
DEFINITION  lambda lysis cassette with terminator
ACCESSION   BBa_K358006
VERSION     BBa_K358006.1  
FEATURES             Location/Qualifiers
     RBS             2..26
                     /label=wt-RBS
     misc_feature    29..352
                     /label=antiholin
     misc_feature    35..352
                     /label=holin
     misc_feature    336..812
                     /label=endolysin
     misc_feature    809..1270
                     /label=Rz
     misc_feature    1029..1211
                     /label=Rz1
     misc_feature    1..1273
                     /label=BBa_K358004
     stem_loop       1293..1336
                     /label=stem_loop
     misc_feature    1282..1361
                     /label=BBa_B0010
     terminator      1282..1361
                     /label=BBa_B0010
     stem_loop       1377..1396
                     /label=T7 TE
     misc_feature    1403..1403
                     /label=stop
     polyA_site      1397..1410
                     /label=polya
     misc_feature    1370..1410
                     /label=BBa_B0012
     terminator      1370..1410
                     /label=BBa_B0012
ORIGIN
        1 gagcaaatcc ccttattggg ggtaagacat gaagatgcca gaaaaacatg acctgttggc
       61 cgccattctc gcggcaaagg aacaaggcat cggggcaatc cttgcgtttg caatggcgta
      121 ccttcgcggc agatataatg gcggtgcgtt tacaaaaaca gtaatcgacg caacgatgtg
      181 cgccattatc gcctggttca ttcgtgacct tctcgacttc gccggactaa gtagcaatct
      241 cgcttatata acgagcgtgt ttatcggcta catcggtact gactcgattg gttcgcttat
      301 caaacgcttc gctgctaaaa aagccggagt agaagatggt agaaatcaat aatcaacgta
      361 aggcgttcct cgatatgctg gcgtggtcgg agggaactga taacggacgt cagaaaacca
      421 gaaatcatgg ttatgacgtc attgtaggcg gagagctatt tactgattac tccgatcacc
      481 ctcgcaaact tgtcacgcta aacccaaaac tcaaatcaac aggcgccgga cgctaccagc
      541 ttctttcccg ttggtgggat gcctaccgca agcagcttgg cctgaaagac ttctctccga
      601 aaagtcagga cgctgtggca ttgcagcaga ttaaggagcg tggcgcttta cctatgattg
      661 atcgtggtga tatccgtcag gcaatcgacc gttgcagcaa tatctgggct tcactgccgg
      721 gcgctggtta tggtcagttc gagcataagg ctgacagcct gattgcaaaa ttcaaagaag
      781 cgggcggaac ggtcagagag attgatgtat gagcagagtc accgcgatta tctccgctct
      841 ggttatctgc atcatcgtct gcctgtcatg ggctgttaat cattaccgtg ataacgccat
      901 tacctacaaa gcccagcgcg acaaaaatgc cagagaactg aagctggcga acgcggcaat
      961 tactgacatg cagatgcgtc agcgtgatgt tgctgcgctc gatgcaaaat acacgaagga
     1021 gttagctgat gctaaagctg aaaatgatgc tctgcgtgat gatgttgccg ctggtcgtcg
     1081 tcggttgcac atcaaagcag tctgtcagtc agtgcgtgaa gccaccaccg cctccggcgt
     1141 ggataatgca gcctcccccc gactggcaga caccgctgaa cgggattatt tcaccctcag
     1201 agagaggctg atcactatgc aaaaacaact ggaaggaacc cagaagtata ttaatgagca
     1261 gtgcagatag agttactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1321 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1381 caccttcggg tgggcctttc tgcgtttata
//