LOCUS       BBa_K358010             1175 bp    DNA     linear       22-Nov-2024
DEFINITION  anti-killer gene with GFP
ACCESSION   BBa_K358010
VERSION     BBa_K358010.1  
FEATURES             Location/Qualifiers
     RBS             2..26
                     /label=wt-RBS
     misc_feature    29..280
                     /label=SdeletionTMD1
     misc_feature    281..289
                     /label=Scar
     misc_feature    1..289
                     /label=BBa_K358012
     RBS             298..312
                     /label=RBS-1\Strong
     RBS             305..309
                     /label=RBS
     misc_feature    298..312
                     /label=BBa_B0030
     RBS             298..312
                     /label=BBa_B0030
     CDS             319..1038
                     /label=GFP protein
     CDS             319..1038
                     /label=BBa_E0040
     stem_loop       1058..1101
                     /label=stem_loop
     misc_feature    1047..1126
                     /label=BBa_B0010
     terminator      1047..1126
                     /label=BBa_B0010
     stem_loop       1142..1161
                     /label=T7 TE
     misc_feature    1168..1168
                     /label=stop
     polyA_site      1162..1175
                     /label=polya
     misc_feature    1135..1175
                     /label=BBa_B0012
     terminator      1135..1175
                     /label=BBa_B0012
     misc_feature    298..1175
                     /label=BBa_E0840
ORIGIN
        1 gagcaaatcc ccttattggg ggtaagacat gaagatgcca gaaaaacatg accgcggcag
       61 atataatggc ggtgcgttta caaaaacagt aatcgacgca acgatgtgcg ccattatcgc
      121 ctggttcatt cgtgaccttc tcgacttcgc cggactaagt agcaatctcg cttatataac
      181 gagcgtgttt atcggctaca tcggtactga ctcgattggt tcgcttatca aacgcttcgc
      241 tgctaaaaaa gccggagtag aagatggtag aaatcaataa tactagagtt actagagatt
      301 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      361 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      421 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      481 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      541 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      601 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      661 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      721 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      781 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      841 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      901 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      961 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     1021 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1081 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1141 tggctcacct tcgggtgggc ctttctgcgt ttata
//