##gff-version 3
##sequence-region BBa_K358010 1 1175
BBa_K358010	.	engineered_region	1	289	.	+	0	ID=BBa_K358012;Name=BBa_K358012
BBa_K358010	.	sequence_feature	281	289	.	+	0	ID=annotation2093184;Name=Scar;Parent=BBa_K358012
BBa_K358010	.	ribosome_entry_site	2	26	.	+	0	ID=annotation2093182;Name=wt-RBS;Parent=BBa_K358012
BBa_K358010	.	sequence_feature	29	280	.	+	0	ID=annotation2093183;Name=SdeletionTMD1;Parent=BBa_K358012
BBa_K358010	.	engineered_region	298	1175	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K358010	.	terminator	1135	1175	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K358010	.	polyA_site	1162	1175	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K358010	.	stem_loop	1142	1161	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K358010	.	stop_codon	1168	1168	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K358010	.	engineered_region	1135	1175	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K358010	.	terminator	1047	1126	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K358010	.	stem_loop	1058	1101	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K358010	.	engineered_region	1047	1126	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K358010	.	ribosome_entry_site	298	312	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K358010	.	engineered_region	298	312	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K358010	.	ribosome_entry_site	305	309	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K358010	.	ribosome_entry_site	298	312	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K358010	.	CDS	319	1038	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K358010	.	CDS	319	1038	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K358010
gagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgaccgcggcagatataatggcggtgcgttt
acaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaa
gtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgctt
cgctgctaaaaaagccggagtagaagatggtagaaatcaataatactagagttactagagattaaagaggagaaatact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata