BBa_K358012 1 BBa_K358012 SΔTMD1 2010-09-18T11:00:00Z 2015-05-08T01:12:12Z λ -Hind Ⅲ digest(product of TaKaRa Bio) TMD1 has been deleted from S gene, which is derived from lambda phage DNA. false false _455_ 0 7170 9 It's complicated false We made point mutation and deletion PCR on S gene. false Ken Kajita annotation2093184 1 Scar range2093184 1 281 289 annotation2093183 1 SdeletionTMD1 range2093183 1 29 280 annotation2093182 1 wt-RBS range2093182 1 2 26 BBa_K358012_sequence 1 gagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatactagagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z