LOCUS       BBa_K358015             1238 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac+anti-killer gene
ACCESSION   BBa_K358015
VERSION     BBa_K358015.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             65..89
                     /label=wt-RBS
     misc_feature    92..343
                     /label=SdeletionTMD1
     misc_feature    344..352
                     /label=Scar
     misc_feature    64..352
                     /label=BBa_K358012
     RBS             361..375
                     /label=RBS-1\Strong
     RBS             368..372
                     /label=RBS
     misc_feature    361..375
                     /label=BBa_B0030
     RBS             361..375
                     /label=BBa_B0030
     CDS             382..1101
                     /label=GFP protein
     CDS             382..1101
                     /label=BBa_E0040
     stem_loop       1121..1164
                     /label=stem_loop
     misc_feature    1110..1189
                     /label=BBa_B0010
     terminator      1110..1189
                     /label=BBa_B0010
     stem_loop       1205..1224
                     /label=T7 TE
     misc_feature    1231..1231
                     /label=stop
     polyA_site      1225..1238
                     /label=polya
     misc_feature    1198..1238
                     /label=BBa_B0012
     terminator      1198..1238
                     /label=BBa_B0012
     misc_feature    361..1238
                     /label=BBa_E0840
     misc_feature    64..1238
                     /label=BBa_K358010
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gaggagcaaa tccccttatt gggggtaaga catgaagatg ccagaaaaac atgaccgcgg
      121 cagatataat ggcggtgcgt ttacaaaaac agtaatcgac gcaacgatgt gcgccattat
      181 cgcctggttc attcgtgacc ttctcgactt cgccggacta agtagcaatc tcgcttatat
      241 aacgagcgtg tttatcggct acatcggtac tgactcgatt ggttcgctta tcaaacgctt
      301 cgctgctaaa aaagccggag tagaagatgg tagaaatcaa taatactaga gttactagag
      361 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      421 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      481 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      541 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      601 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      661 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      721 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      781 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      841 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      901 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      961 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1021 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1081 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1141 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1201 cactggctca ccttcgggtg ggcctttctg cgtttata
//