BBa_K358015
type: ComponentDefinition
persistent identity: BBa K358015
display id: BBa_K358015
version: 1
was generated by: iGEM to SBOL conversion
description: pLac+anti-killer gene
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: BBa_R0011, BBa_K358010
owned by: james
owned by: myers
mutable description: The anti-killer gene with GFP[BBa_K358010] is expressed under the control of lac promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: non
star: false
creator: Ken Kajita
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K358010
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_K358010
sequence: https://synbiohub.org/public/igem/BBa_K358015_sequence/1

BBa_K358010
type: Component
persistent identity: component2224322
display id: component2224322
version: 1
top level: BBa_K358015
definition: BBa_K358010
access: public

BBa_R0011
type: Component
persistent identity: component2224302
display id: component2224302
version: 1
top level: BBa_K358015
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2224302
display id: annotation2224302
version: 1
top level: BBa_K358015
location: https://synbiohub.org/public/igem/BBa_K358015/annotation2224302/range2224302/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K358015/annotation2224302/range2224302/1
type: Range
persistent identity: range2224302
display id: range2224302
version: 1
top level: BBa_K358015
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K358010
type: SequenceAnnotation
persistent identity: annotation2224322
display id: annotation2224322
version: 1
top level: BBa_K358015
location: https://synbiohub.org/public/igem/BBa_K358015/annotation2224322/range2224322/1
component: BBa_K358010

https://synbiohub.org/public/igem/BBa_K358015/annotation2224322/range2224322/1
type: Range
persistent identity: range2224322
display id: range2224322
version: 1
top level: BBa_K358015
direction: direction/reverse
start: 64
end: 1238
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K358010
type: ComponentDefinition
persistent identity: BBa K358010
display id: BBa_K358010
version: 1
was generated by: iGEM to SBOL conversion
description: anti-killer gene with GFP
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: λ -Hind Ⅲ digest (product of TaKaRa Bio) and BBa_E0240
owned by: james
owned by: myers
mutable description: It codes SΔTMD1 as the anti-killer gene, which is derived from λ phage DNA and for the lambda lysis cassette[BBa_K358005]: the killer gene.
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We made point mutation and deletion PCR on S gene to prepare this part.
star: false
creator: Ken Kajita
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_E0840
component: BBa_K358012
sequence annotation: BBa_K358012
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K358010_sequence/1

BBa_E0840
type: Component
persistent identity: component2222554
display id: component2222554
version: 1
top level: BBa_K358010
definition: GFP genera
access: public

BBa_K358012
type: Component
persistent identity: component2222543
display id: component2222543
version: 1
top level: BBa_K358010
definition: BBa_K358012
access: public

BBa_K358012
type: SequenceAnnotation
persistent identity: annotation2222543
display id: annotation2222543
version: 1
top level: BBa_K358010
location: https://synbiohub.org/public/igem/BBa_K358010/annotation2222543/range2222543/1
component: BBa_K358012

https://synbiohub.org/public/igem/BBa_K358010/annotation2222543/range2222543/1
type: Range
persistent identity: range2222543
display id: range2222543
version: 1
top level: BBa_K358010
direction: direction/reverse
start: 1
end: 289
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2222554
display id: annotation2222554
version: 1
top level: BBa_K358010
location: https://synbiohub.org/public/igem/BBa_K358010/annotation2222554/range2222554/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K358010/annotation2222554/range2222554/1
type: Range
persistent identity: range2222554
display id: range2222554
version: 1
top level: BBa_K358010
direction: direction/reverse
start: 298
end: 1175
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0030.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0030
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
definition: BBa_B0030
access: public

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
definition: BBa_B0012
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
top level: GFP genera
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
top level: GFP genera
direction: direction/forward
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
top level: GFP genera
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
top level: GFP genera
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_K358012
type: ComponentDefinition
persistent identity: BBa K358012
display id: BBa_K358012
version: 1
was generated by: iGEM to SBOL conversion
description: SΔTMD1
created: 2010-09-18T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: λ -Hind Ⅲ digest(product of TaKaRa Bio)
owned by: james
owned by: myers
mutable description: TMD1 has been deleted from S gene, which is derived from lambda phage DNA.
discontinued: false
dominant: false
group u list: _455_
m user id: 0
owner id: 7170
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We made point mutation and deletion PCR on S gene.
star: false
creator: Ken Kajita
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: wt-RBS
sequence annotation: SdeletionTMD1
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K358012_sequence/1

wt-RBS
type: SequenceAnnotation
persistent identity: annotation2093182
display id: annotation2093182
version: 1
top level: BBa_K358012
location: https://synbiohub.org/public/igem/BBa_K358012/annotation2093182/range2093182/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K358012/annotation2093182/range2093182/1
type: Range
persistent identity: range2093182
display id: range2093182
version: 1
top level: BBa_K358012
direction: direction/forward
start: 2
end: 26
orientation: inline

SdeletionTMD1
type: SequenceAnnotation
persistent identity: annotation2093183
display id: annotation2093183
version: 1
top level: BBa_K358012
location: https://synbiohub.org/public/igem/BBa_K358012/annotation2093183/range2093183/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K358012/annotation2093183/range2093183/1
type: Range
persistent identity: range2093183
display id: range2093183
version: 1
top level: BBa_K358012
direction: direction/forward
start: 29
end: 280
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2093184
display id: annotation2093184
version: 1
top level: BBa_K358012
location: https://synbiohub.org/public/igem/BBa_K358012/annotation2093184/range2093184/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K358012/annotation2093184/range2093184/1
type: Range
persistent identity: range2093184
display id: range2093184
version: 1
top level: BBa_K358012
direction: direction/forward
start: 281
end: 289
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K358015_sequence/1
type: Sequence
persistent identity: BBa K358015 sequence
display id: BBa_K358015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatactagagttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K358010_sequence/1
type: Sequence
persistent identity: BBa K358010 sequence
display id: BBa_K358010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatactagagttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0840_sequence/1
type: Sequence
persistent identity: BBa E0840 sequence
display id: BBa_E0840_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K358012_sequence/1
type: Sequence
persistent identity: BBa K358012 sequence
display id: BBa_K358012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatactagagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z