##gff-version 3
##sequence-region BBa_K358016 1 1281
BBa_K358016	.	promoter	1247	1281	.	+	0	ID=BBa_J23105;Name=BBa_J23105
BBa_K358016	.	engineered_region	1	1238	.	+	0	ID=BBa_K358015;Name=BBa_K358015
BBa_K358016	.	engineered_region	64	1238	.	+	0	ID=BBa_K358010;Name=BBa_K358010;Parent=BBa_K358015
BBa_K358016	.	engineered_region	361	1238	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K358010
BBa_K358016	.	ribosome_entry_site	361	375	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K358016	.	ribosome_entry_site	361	375	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K358016	.	engineered_region	361	375	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K358016	.	ribosome_entry_site	368	372	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K358016	.	CDS	382	1101	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K358016	.	CDS	382	1101	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K358016	.	terminator	1110	1189	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K358016	.	stem_loop	1121	1164	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K358016	.	engineered_region	1110	1189	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K358016	.	terminator	1198	1238	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K358016	.	polyA_site	1225	1238	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K358016	.	stop_codon	1231	1231	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K358016	.	engineered_region	1198	1238	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K358016	.	stem_loop	1205	1224	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K358016	.	engineered_region	64	352	.	+	0	ID=BBa_K358012;Name=BBa_K358012;Parent=BBa_K358010
BBa_K358016	.	ribosome_entry_site	65	89	.	+	0	ID=annotation2093182;Name=wt-RBS;Parent=BBa_K358012
BBa_K358016	.	sequence_feature	344	352	.	+	0	ID=annotation2093184;Name=Scar;Parent=BBa_K358012
BBa_K358016	.	sequence_feature	92	343	.	+	0	ID=annotation2093183;Name=SdeletionTMD1;Parent=BBa_K358012
BBa_K358016	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K358015
BBa_K358016	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K358016	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K358016	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K358016	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K358016	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
>BBa_K358016
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggagcaaatccccttat
tgggggtaagacatgaagatgccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcg
acgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgctta
tataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagcc
ggagtagaagatggtagaaatcaataatactagagttactagagattaaagaggagaaatactagatgcgtaaaggaga
agaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtgga
gagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggc
caacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttttt
caagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagt
taacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagag
accacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtc
ctaggtactatgctagc