LOCUS       BBa_K358018             1281 bp    DNA     linear       17-May-2024
DEFINITION  Low promoter+anti-killergene+lacP
ACCESSION   BBa_K358018
VERSION     BBa_K358018.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23105
     RBS             45..69
                     /label=wt-RBS
     misc_feature    72..323
                     /label=SdeletionTMD1
     misc_feature    324..332
                     /label=Scar
     misc_feature    44..332
                     /label=BBa_K358012
     RBS             341..355
                     /label=RBS-1\Strong
     RBS             348..352
                     /label=RBS
     misc_feature    341..355
                     /label=BBa_B0030
     RBS             341..355
                     /label=BBa_B0030
     CDS             362..1081
                     /label=GFP protein
     CDS             362..1081
                     /label=BBa_E0040
     stem_loop       1101..1144
                     /label=stem_loop
     misc_feature    1090..1169
                     /label=BBa_B0010
     terminator      1090..1169
                     /label=BBa_B0010
     stem_loop       1185..1204
                     /label=T7 TE
     misc_feature    1211..1211
                     /label=stop
     polyA_site      1205..1218
                     /label=polya
     misc_feature    1178..1218
                     /label=BBa_B0012
     terminator      1178..1218
                     /label=BBa_B0012
     misc_feature    341..1218
                     /label=BBa_E0840
     misc_feature    44..1218
                     /label=BBa_K358010
     misc_feature    1229..1245
                     /label=lac O1
     promoter        1246..1251
                     /label=-35
     misc_feature    1252..1268
                     /label=lac O1
     promoter        1269..1274
                     /label=-10
     misc_feature    1227..1280
                     /label=BBa_R0011
     promoter        1227..1280
                     /label=BBa_R0011
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gaggagcaaa tccccttatt
       61 gggggtaaga catgaagatg ccagaaaaac atgaccgcgg cagatataat ggcggtgcgt
      121 ttacaaaaac agtaatcgac gcaacgatgt gcgccattat cgcctggttc attcgtgacc
      181 ttctcgactt cgccggacta agtagcaatc tcgcttatat aacgagcgtg tttatcggct
      241 acatcggtac tgactcgatt ggttcgctta tcaaacgctt cgctgctaaa aaagccggag
      301 tagaagatgg tagaaatcaa taatactaga gttactagag attaaagagg agaaatacta
      361 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      421 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      481 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      541 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      601 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      661 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      721 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      781 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      841 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      901 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      961 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     1021 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     1081 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1141 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1201 ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac attgtgagcg
     1261 gataacaaga tactgagcac a
//