##gff-version 3
##sequence-region BBa_K358018 1 1280
BBa_K358018	.	engineered_region	44	1218	.	+	0	ID=BBa_K358010;Name=BBa_K358010
BBa_K358018	.	engineered_region	341	1218	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K358010
BBa_K358018	.	CDS	362	1081	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K358018	.	CDS	362	1081	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K358018	.	ribosome_entry_site	341	355	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K358018	.	engineered_region	341	355	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K358018	.	ribosome_entry_site	348	352	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K358018	.	ribosome_entry_site	341	355	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K358018	.	terminator	1178	1218	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K358018	.	stop_codon	1211	1211	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K358018	.	polyA_site	1205	1218	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K358018	.	stem_loop	1185	1204	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K358018	.	engineered_region	1178	1218	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K358018	.	terminator	1090	1169	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K358018	.	stem_loop	1101	1144	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K358018	.	engineered_region	1090	1169	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K358018	.	engineered_region	44	332	.	+	0	ID=BBa_K358012;Name=BBa_K358012;Parent=BBa_K358010
BBa_K358018	.	sequence_feature	324	332	.	+	0	ID=annotation2093184;Name=Scar;Parent=BBa_K358012
BBa_K358018	.	ribosome_entry_site	45	69	.	+	0	ID=annotation2093182;Name=wt-RBS;Parent=BBa_K358012
BBa_K358018	.	sequence_feature	72	323	.	+	0	ID=annotation2093183;Name=SdeletionTMD1;Parent=BBa_K358012
BBa_K358018	.	promoter	1227	1280	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K358018	.	promoter	1269	1274	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K358018	.	operator	1252	1268	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K358018	.	operator	1229	1245	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K358018	.	promoter	1246	1251	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K358018	.	engineered_region	1227	1280	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K358018	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105
>BBa_K358018
tttacggctagctcagtcctaggtactatgctagctactagaggagcaaatccccttattgggggtaagacatgaagat
gccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccatt
atcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcg
gctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaa
tcaataatactagagttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttg
tcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaac
atacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttc
ggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaag
gttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtga
tacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatac
aactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacaca
acattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacc
agacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgag
tttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggata
acaagatactgagcaca