LOCUS       BBa_K358019             1473 bp    DNA     linear       25-Nov-2024
DEFINITION  Lysis cassette regulated by lacP
ACCESSION   BBa_K358019
VERSION     BBa_K358019.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             65..89
                     /label=wt-RBS
     misc_feature    92..415
                     /label=antiholin
     misc_feature    98..415
                     /label=holin
     misc_feature    399..875
                     /label=endolysin
     misc_feature    872..1333
                     /label=Rz
     misc_feature    1092..1274
                     /label=Rz1
     misc_feature    64..1336
                     /label=BBa_K358004
     stem_loop       1356..1399
                     /label=stem_loop
     misc_feature    1345..1424
                     /label=BBa_B0010
     terminator      1345..1424
                     /label=BBa_B0010
     stem_loop       1440..1459
                     /label=T7 TE
     misc_feature    1466..1466
                     /label=stop
     polyA_site      1460..1473
                     /label=polya
     misc_feature    1433..1473
                     /label=BBa_B0012
     terminator      1433..1473
                     /label=BBa_B0012
     misc_feature    64..1473
                     /label=BBa_K358006
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gaggagcaaa tccccttatt gggggtaaga catgaagatg ccagaaaaac atgacctgtt
      121 ggccgccatt ctcgcggcaa aggaacaagg catcggggca atccttgcgt ttgcaatggc
      181 gtaccttcgc ggcagatata atggcggtgc gtttacaaaa acagtaatcg acgcaacgat
      241 gtgcgccatt atcgcctggt tcattcgtga ccttctcgac ttcgccggac taagtagcaa
      301 tctcgcttat ataacgagcg tgtttatcgg ctacatcggt actgactcga ttggttcgct
      361 tatcaaacgc ttcgctgcta aaaaagccgg agtagaagat ggtagaaatc aataatcaac
      421 gtaaggcgtt cctcgatatg ctggcgtggt cggagggaac tgataacgga cgtcagaaaa
      481 ccagaaatca tggttatgac gtcattgtag gcggagagct atttactgat tactccgatc
      541 accctcgcaa acttgtcacg ctaaacccaa aactcaaatc aacaggcgcc ggacgctacc
      601 agcttctttc ccgttggtgg gatgcctacc gcaagcagct tggcctgaaa gacttctctc
      661 cgaaaagtca ggacgctgtg gcattgcagc agattaagga gcgtggcgct ttacctatga
      721 ttgatcgtgg tgatatccgt caggcaatcg accgttgcag caatatctgg gcttcactgc
      781 cgggcgctgg ttatggtcag ttcgagcata aggctgacag cctgattgca aaattcaaag
      841 aagcgggcgg aacggtcaga gagattgatg tatgagcaga gtcaccgcga ttatctccgc
      901 tctggttatc tgcatcatcg tctgcctgtc atgggctgtt aatcattacc gtgataacgc
      961 cattacctac aaagcccagc gcgacaaaaa tgccagagaa ctgaagctgg cgaacgcggc
     1021 aattactgac atgcagatgc gtcagcgtga tgttgctgcg ctcgatgcaa aatacacgaa
     1081 ggagttagct gatgctaaag ctgaaaatga tgctctgcgt gatgatgttg ccgctggtcg
     1141 tcgtcggttg cacatcaaag cagtctgtca gtcagtgcgt gaagccacca ccgcctccgg
     1201 cgtggataat gcagcctccc cccgactggc agacaccgct gaacgggatt atttcaccct
     1261 cagagagagg ctgatcacta tgcaaaaaca actggaagga acccagaagt atattaatga
     1321 gcagtgcaga tagagttact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1381 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1441 gctcaccttc gggtgggcct ttctgcgttt ata
//