##gff-version 3
##sequence-region BBa_K358020 1 2699
BBa_K358020	.	engineered_region	1290	2699	.	+	0	ID=BBa_K358006;Name=BBa_K358006
BBa_K358020	.	terminator	2571	2650	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K358006
BBa_K358020	.	engineered_region	2571	2650	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K358020	.	stem_loop	2582	2625	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K358020	.	engineered_region	1290	2562	.	+	0	ID=BBa_K358004;Name=BBa_K358004;Parent=BBa_K358006
BBa_K358020	.	ribosome_entry_site	1291	1315	.	+	0	ID=annotation2080446;Name=wt-RBS;Parent=BBa_K358004
BBa_K358020	.	sequence_feature	2318	2500	.	+	0	ID=annotation2080451;Name=Rz1;Parent=BBa_K358004
BBa_K358020	.	sequence_feature	1318	1641	.	+	0	ID=annotation2080447;Name=antiholin;Parent=BBa_K358004
BBa_K358020	.	sequence_feature	2098	2559	.	+	0	ID=annotation2080450;Name=Rz;Parent=BBa_K358004
BBa_K358020	.	sequence_feature	1625	2101	.	+	0	ID=annotation2080449;Name=endolysin;Parent=BBa_K358004
BBa_K358020	.	sequence_feature	1324	1641	.	+	0	ID=annotation2080448;Name=holin;Parent=BBa_K358004
BBa_K358020	.	terminator	2659	2699	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K358006
BBa_K358020	.	engineered_region	2659	2699	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K358020	.	polyA_site	2686	2699	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K358020	.	stop_codon	2692	2692	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K358020	.	stem_loop	2666	2685	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K358020	.	engineered_region	1	1281	.	+	0	ID=BBa_K358016;Name=BBa_K358016
BBa_K358020	.	engineered_region	1	1238	.	+	0	ID=BBa_K358015;Name=BBa_K358015;Parent=BBa_K358016
BBa_K358020	.	engineered_region	64	1238	.	+	0	ID=BBa_K358010;Name=BBa_K358010;Parent=BBa_K358015
BBa_K358020	.	engineered_region	361	1238	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K358010
BBa_K358020	.	terminator	1198	1238	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K358020	.	engineered_region	1198	1238	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K358020	.	polyA_site	1225	1238	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K358020	.	stop_codon	1231	1231	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K358020	.	stem_loop	1205	1224	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K358020	.	terminator	1110	1189	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K358020	.	engineered_region	1110	1189	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K358020	.	stem_loop	1121	1164	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K358020	.	ribosome_entry_site	361	375	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K358020	.	ribosome_entry_site	361	375	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K358020	.	engineered_region	361	375	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K358020	.	ribosome_entry_site	368	372	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K358020	.	CDS	382	1101	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K358020	.	CDS	382	1101	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K358020	.	engineered_region	64	352	.	+	0	ID=BBa_K358012;Name=BBa_K358012;Parent=BBa_K358010
BBa_K358020	.	sequence_feature	92	343	.	+	0	ID=annotation2093183;Name=SdeletionTMD1;Parent=BBa_K358012
BBa_K358020	.	sequence_feature	344	352	.	+	0	ID=annotation2093184;Name=Scar;Parent=BBa_K358012
BBa_K358020	.	ribosome_entry_site	65	89	.	+	0	ID=annotation2093182;Name=wt-RBS;Parent=BBa_K358012
BBa_K358020	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K358015
BBa_K358020	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K358020	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K358020	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K358020	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K358020	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K358020	.	promoter	1247	1281	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_K358016
>BBa_K358020
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggagcaaatccccttat
tgggggtaagacatgaagatgccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcg
acgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgctta
tataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagcc
ggagtagaagatggtagaaatcaataatactagagttactagagattaaagaggagaaatactagatgcgtaaaggaga
agaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtgga
gagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggc
caacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttttt
caagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagt
taacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagag
accacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtc
ctaggtactatgctagctactagaggagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgacct
gttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcaga
tataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttc
tcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattgg
ttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatcaacgtaaggcgttcct
cgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggc
ggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccg
gacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtca
ggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatc
gaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattg
caaaattcaaagaagcgggcggaacggtcagagagattgatgtatgagcagagtcaccgcgattatctccgctctggtt
atctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgaca
aaaatgccagagaactgaagctggcgaacgcggcaattactgacatgcagatgcgtcagcgtgatgttgctgcgctcga
tgcaaaatacacgaaggagttagctgatgctaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgt
cggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccaccgcctccggcgtggataatgcagcctcccccc
gactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaac
ccagaagtatattaatgagcagtgcagatagagttactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata