LOCUS       BBa_K358021             2699 bp    DNA     linear       15-Nov-2024
DEFINITION  Lysisbox -type2-
ACCESSION   BBa_K358021
VERSION     BBa_K358021.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23105
     RBS             45..69
                     /label=wt-RBS
     misc_feature    72..323
                     /label=SdeletionTMD1
     misc_feature    324..332
                     /label=Scar
     misc_feature    44..332
                     /label=BBa_K358012
     RBS             341..355
                     /label=RBS-1\Strong
     RBS             348..352
                     /label=RBS
     misc_feature    341..355
                     /label=BBa_B0030
     RBS             341..355
                     /label=BBa_B0030
     CDS             362..1081
                     /label=GFP protein
     CDS             362..1081
                     /label=BBa_E0040
     stem_loop       1101..1144
                     /label=stem_loop
     misc_feature    1090..1169
                     /label=BBa_B0010
     terminator      1090..1169
                     /label=BBa_B0010
     stem_loop       1185..1204
                     /label=T7 TE
     misc_feature    1211..1211
                     /label=stop
     polyA_site      1205..1218
                     /label=polya
     misc_feature    1178..1218
                     /label=BBa_B0012
     terminator      1178..1218
                     /label=BBa_B0012
     misc_feature    341..1218
                     /label=BBa_E0840
     misc_feature    44..1218
                     /label=BBa_K358010
     misc_feature    1229..1245
                     /label=lac O1
     promoter        1246..1251
                     /label=-35
     misc_feature    1252..1268
                     /label=lac O1
     promoter        1269..1274
                     /label=-10
     misc_feature    1227..1280
                     /label=BBa_R0011
     promoter        1227..1280
                     /label=BBa_R0011
     misc_feature    1..1281
                     /label=BBa_K358018
     RBS             1291..1315
                     /label=wt-RBS
     misc_feature    1318..1641
                     /label=antiholin
     misc_feature    1324..1641
                     /label=holin
     misc_feature    1625..2101
                     /label=endolysin
     misc_feature    2098..2559
                     /label=Rz
     misc_feature    2318..2500
                     /label=Rz1
     misc_feature    1290..2562
                     /label=BBa_K358004
     stem_loop       2582..2625
                     /label=stem_loop
     misc_feature    2571..2650
                     /label=BBa_B0010
     terminator      2571..2650
                     /label=BBa_B0010
     stem_loop       2666..2685
                     /label=T7 TE
     misc_feature    2692..2692
                     /label=stop
     polyA_site      2686..2699
                     /label=polya
     misc_feature    2659..2699
                     /label=BBa_B0012
     terminator      2659..2699
                     /label=BBa_B0012
     misc_feature    1290..2699
                     /label=BBa_K358006
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gaggagcaaa tccccttatt
       61 gggggtaaga catgaagatg ccagaaaaac atgaccgcgg cagatataat ggcggtgcgt
      121 ttacaaaaac agtaatcgac gcaacgatgt gcgccattat cgcctggttc attcgtgacc
      181 ttctcgactt cgccggacta agtagcaatc tcgcttatat aacgagcgtg tttatcggct
      241 acatcggtac tgactcgatt ggttcgctta tcaaacgctt cgctgctaaa aaagccggag
      301 tagaagatgg tagaaatcaa taatactaga gttactagag attaaagagg agaaatacta
      361 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      421 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      481 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      541 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      601 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      661 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      721 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      781 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      841 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      901 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      961 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     1021 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     1081 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1141 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1201 ggcctttctg cgtttatata ctagagaatt gtgagcggat aacaattgac attgtgagcg
     1261 gataacaaga tactgagcac atactagagg agcaaatccc cttattgggg gtaagacatg
     1321 aagatgccag aaaaacatga cctgttggcc gccattctcg cggcaaagga acaaggcatc
     1381 ggggcaatcc ttgcgtttgc aatggcgtac cttcgcggca gatataatgg cggtgcgttt
     1441 acaaaaacag taatcgacgc aacgatgtgc gccattatcg cctggttcat tcgtgacctt
     1501 ctcgacttcg ccggactaag tagcaatctc gcttatataa cgagcgtgtt tatcggctac
     1561 atcggtactg actcgattgg ttcgcttatc aaacgcttcg ctgctaaaaa agccggagta
     1621 gaagatggta gaaatcaata atcaacgtaa ggcgttcctc gatatgctgg cgtggtcgga
     1681 gggaactgat aacggacgtc agaaaaccag aaatcatggt tatgacgtca ttgtaggcgg
     1741 agagctattt actgattact ccgatcaccc tcgcaaactt gtcacgctaa acccaaaact
     1801 caaatcaaca ggcgccggac gctaccagct tctttcccgt tggtgggatg cctaccgcaa
     1861 gcagcttggc ctgaaagact tctctccgaa aagtcaggac gctgtggcat tgcagcagat
     1921 taaggagcgt ggcgctttac ctatgattga tcgtggtgat atccgtcagg caatcgaccg
     1981 ttgcagcaat atctgggctt cactgccggg cgctggttat ggtcagttcg agcataaggc
     2041 tgacagcctg attgcaaaat tcaaagaagc gggcggaacg gtcagagaga ttgatgtatg
     2101 agcagagtca ccgcgattat ctccgctctg gttatctgca tcatcgtctg cctgtcatgg
     2161 gctgttaatc attaccgtga taacgccatt acctacaaag cccagcgcga caaaaatgcc
     2221 agagaactga agctggcgaa cgcggcaatt actgacatgc agatgcgtca gcgtgatgtt
     2281 gctgcgctcg atgcaaaata cacgaaggag ttagctgatg ctaaagctga aaatgatgct
     2341 ctgcgtgatg atgttgccgc tggtcgtcgt cggttgcaca tcaaagcagt ctgtcagtca
     2401 gtgcgtgaag ccaccaccgc ctccggcgtg gataatgcag cctccccccg actggcagac
     2461 accgctgaac gggattattt caccctcaga gagaggctga tcactatgca aaaacaactg
     2521 gaaggaaccc agaagtatat taatgagcag tgcagataga gttactagag ccaggcatca
     2581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2641 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//