##gff-version 3
##sequence-region BBa_K358021 1 2699
BBa_K358021	.	engineered_region	1	1281	.	+	0	ID=BBa_K358018;Name=BBa_K358018
BBa_K358021	.	engineered_region	44	1218	.	+	0	ID=BBa_K358010;Name=BBa_K358010;Parent=BBa_K358018
BBa_K358021	.	engineered_region	341	1218	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K358010
BBa_K358021	.	ribosome_entry_site	341	355	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K358021	.	ribosome_entry_site	341	355	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K358021	.	ribosome_entry_site	348	352	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K358021	.	engineered_region	341	355	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K358021	.	CDS	362	1081	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K358021	.	CDS	362	1081	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K358021	.	terminator	1090	1169	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K358021	.	stem_loop	1101	1144	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K358021	.	engineered_region	1090	1169	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K358021	.	terminator	1178	1218	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K358021	.	polyA_site	1205	1218	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K358021	.	stop_codon	1211	1211	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K358021	.	stem_loop	1185	1204	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K358021	.	engineered_region	1178	1218	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K358021	.	engineered_region	44	332	.	+	0	ID=BBa_K358012;Name=BBa_K358012;Parent=BBa_K358010
BBa_K358021	.	ribosome_entry_site	45	69	.	+	0	ID=annotation2093182;Name=wt-RBS;Parent=BBa_K358012
BBa_K358021	.	sequence_feature	324	332	.	+	0	ID=annotation2093184;Name=Scar;Parent=BBa_K358012
BBa_K358021	.	sequence_feature	72	323	.	+	0	ID=annotation2093183;Name=SdeletionTMD1;Parent=BBa_K358012
BBa_K358021	.	promoter	1227	1280	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K358018
BBa_K358021	.	promoter	1246	1251	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K358021	.	engineered_region	1227	1280	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K358021	.	operator	1252	1268	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K358021	.	operator	1229	1245	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K358021	.	promoter	1269	1274	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K358021	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_K358018
BBa_K358021	.	engineered_region	1290	2699	.	+	0	ID=BBa_K358006;Name=BBa_K358006
BBa_K358021	.	terminator	2571	2650	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K358006
BBa_K358021	.	stem_loop	2582	2625	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K358021	.	engineered_region	2571	2650	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K358021	.	engineered_region	1290	2562	.	+	0	ID=BBa_K358004;Name=BBa_K358004;Parent=BBa_K358006
BBa_K358021	.	sequence_feature	2098	2559	.	+	0	ID=annotation2080450;Name=Rz;Parent=BBa_K358004
BBa_K358021	.	sequence_feature	1318	1641	.	+	0	ID=annotation2080447;Name=antiholin;Parent=BBa_K358004
BBa_K358021	.	ribosome_entry_site	1291	1315	.	+	0	ID=annotation2080446;Name=wt-RBS;Parent=BBa_K358004
BBa_K358021	.	sequence_feature	1324	1641	.	+	0	ID=annotation2080448;Name=holin;Parent=BBa_K358004
BBa_K358021	.	sequence_feature	2318	2500	.	+	0	ID=annotation2080451;Name=Rz1;Parent=BBa_K358004
BBa_K358021	.	sequence_feature	1625	2101	.	+	0	ID=annotation2080449;Name=endolysin;Parent=BBa_K358004
BBa_K358021	.	terminator	2659	2699	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K358006
BBa_K358021	.	polyA_site	2686	2699	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K358021	.	stop_codon	2692	2692	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K358021	.	stem_loop	2666	2685	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K358021	.	engineered_region	2659	2699	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K358021
tttacggctagctcagtcctaggtactatgctagctactagaggagcaaatccccttattgggggtaagacatgaagat
gccagaaaaacatgaccgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccatt
atcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcg
gctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaa
tcaataatactagagttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttg
tcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaac
atacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttc
ggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaag
gttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtga
tacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatac
aactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacaca
acattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacc
agacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgag
tttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggata
acaagatactgagcacatactagaggagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatgacct
gttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcaga
tataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttc
tcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattgg
ttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataatcaacgtaaggcgttcct
cgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggc
ggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccg
gacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtca
ggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatc
gaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattg
caaaattcaaagaagcgggcggaacggtcagagagattgatgtatgagcagagtcaccgcgattatctccgctctggtt
atctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgaca
aaaatgccagagaactgaagctggcgaacgcggcaattactgacatgcagatgcgtcagcgtgatgttgctgcgctcga
tgcaaaatacacgaaggagttagctgatgctaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgt
cggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccaccgcctccggcgtggataatgcagcctcccccc
gactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaac
ccagaagtatattaatgagcagtgcagatagagttactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttata