LOCUS       BBa_K358022             1401 bp    DNA     linear       21-Dec-2024
DEFINITION  lacP + SΔTMD1RRz
ACCESSION   BBa_K358022
VERSION     BBa_K358022.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             65..89
                     /label=wt-RBS
     misc_feature    92..343
                     /label=SΔTMD1
     misc_feature    327..803
                     /label=endolysin
     misc_feature    800..1261
                     /label=Rz
     misc_feature    1020..1202
                     /label=Rz1
     misc_feature    64..1264
                     /label=BBa_K358014
     stem_loop       1284..1327
                     /label=stem_loop
     misc_feature    1273..1352
                     /label=BBa_B0010
     terminator      1273..1352
                     /label=BBa_B0010
     stem_loop       1368..1387
                     /label=T7 TE
     misc_feature    1394..1394
                     /label=stop
     polyA_site      1388..1401
                     /label=polya
     misc_feature    1361..1401
                     /label=BBa_B0012
     terminator      1361..1401
                     /label=BBa_B0012
     terminator      1273..1401
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gaggagcaaa tccccttatt gggggtaaga catgaagatg ccagaaaaac atgaccgcgg
      121 cagatataat ggcggtgcgt ttacaaaaac agtaatcgac gcaacgatgt gcgccattat
      181 cgcctggttc attcgtgacc ttctcgactt cgccggacta agtagcaatc tcgcttatat
      241 aacgagcgtg tttatcggct acatcggtac tgactcgatt ggttcgctta tcaaacgctt
      301 cgctgctaaa aaagccggag tagaagatgg tagaaatcaa taatcaacgt aaggcgttcc
      361 tcgatatgct ggcgtggtcg gagggaactg ataacggacg tcagaaaacc agaaatcatg
      421 gttatgacgt cattgtaggc ggagagctat ttactgatta ctccgatcac cctcgcaaac
      481 ttgtcacgct aaacccaaaa ctcaaatcaa caggcgccgg acgctaccag cttctttccc
      541 gttggtggga tgcctaccgc aagcagcttg gcctgaaaga cttctctccg aaaagtcagg
      601 acgctgtggc attgcagcag attaaggagc gtggcgcttt acctatgatt gatcgtggtg
      661 atatccgtca ggcaatcgac cgttgcagca atatctgggc ttcactgccg ggcgctggtt
      721 atggtcagtt cgagcataag gctgacagcc tgattgcaaa attcaaagaa gcgggcggaa
      781 cggtcagaga gattgatgta tgagcagagt caccgcgatt atctccgctc tggttatctg
      841 catcatcgtc tgcctgtcat gggctgttaa tcattaccgt gataacgcca ttacctacaa
      901 agcccagcgc gacaaaaatg ccagagaact gaagctggcg aacgcggcaa ttactgacat
      961 gcagatgcgt cagcgtgatg ttgctgcgct cgatgcaaaa tacacgaagg agttagctga
     1021 tgctaaagct gaaaatgatg ctctgcgtga tgatgttgcc gctggtcgtc gtcggttgca
     1081 catcaaagca gtctgtcagt cagtgcgtga agccaccacc gcctccggcg tggataatgc
     1141 agcctccccc cgactggcag acaccgctga acgggattat ttcaccctca gagagaggct
     1201 gatcactatg caaaaacaac tggaaggaac ccagaagtat attaatgagc agtgcagata
     1261 gagttactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1321 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1381 gtgggccttt ctgcgtttat a
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