LOCUS       BBa_K358023             2699 bp    DNA     linear       22-Dec-2024
DEFINITION  Lysisbox -type1- ver.2
ACCESSION   BBa_K358023
VERSION     BBa_K358023.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             65..89
                     /label=wt-RBS
     misc_feature    92..343
                     /label=SdeletionTMD1
     misc_feature    344..352
                     /label=Scar
     misc_feature    64..352
                     /label=BBa_K358012
     RBS             361..375
                     /label=RBS-1\Strong
     RBS             368..372
                     /label=RBS
     misc_feature    361..375
                     /label=BBa_B0030
     RBS             361..375
                     /label=BBa_B0030
     CDS             382..1101
                     /label=GFP protein
     CDS             382..1101
                     /label=BBa_E0040
     stem_loop       1121..1164
                     /label=stem_loop
     misc_feature    1110..1189
                     /label=BBa_B0010
     terminator      1110..1189
                     /label=BBa_B0010
     stem_loop       1205..1224
                     /label=T7 TE
     misc_feature    1231..1231
                     /label=stop
     polyA_site      1225..1238
                     /label=polya
     misc_feature    1198..1238
                     /label=BBa_B0012
     terminator      1198..1238
                     /label=BBa_B0012
     misc_feature    361..1238
                     /label=BBa_E0840
     misc_feature    64..1238
                     /label=BBa_K358010
     misc_feature    1..1238
                     /label=BBa_K358015
     promoter        1247..1281
                     /label=BBa_J23100
     RBS             1291..1315
                     /label=wt-RBS
     misc_feature    1318..1641
                     /label=antiholin
     misc_feature    1324..1641
                     /label=holin
     misc_feature    1625..2101
                     /label=endolysin
     misc_feature    2098..2559
                     /label=Rz
     misc_feature    2318..2500
                     /label=Rz1
     misc_feature    1290..2562
                     /label=BBa_K358004
     stem_loop       2582..2625
                     /label=stem_loop
     misc_feature    2571..2650
                     /label=BBa_B0010
     terminator      2571..2650
                     /label=BBa_B0010
     stem_loop       2666..2685
                     /label=T7 TE
     misc_feature    2692..2692
                     /label=stop
     polyA_site      2686..2699
                     /label=polya
     misc_feature    2659..2699
                     /label=BBa_B0012
     terminator      2659..2699
                     /label=BBa_B0012
     misc_feature    1290..2699
                     /label=BBa_K358006
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gaggagcaaa tccccttatt gggggtaaga catgaagatg ccagaaaaac atgaccgcgg
      121 cagatataat ggcggtgcgt ttacaaaaac agtaatcgac gcaacgatgt gcgccattat
      181 cgcctggttc attcgtgacc ttctcgactt cgccggacta agtagcaatc tcgcttatat
      241 aacgagcgtg tttatcggct acatcggtac tgactcgatt ggttcgctta tcaaacgctt
      301 cgctgctaaa aaagccggag tagaagatgg tagaaatcaa taatactaga gttactagag
      361 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      421 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      481 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      541 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      601 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      661 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      721 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      781 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      841 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      901 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      961 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1021 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1081 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1141 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1201 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cggctagctc
     1261 agtcctaggt acagtgctag ctactagagg agcaaatccc cttattgggg gtaagacatg
     1321 aagatgccag aaaaacatga cctgttggcc gccattctcg cggcaaagga acaaggcatc
     1381 ggggcaatcc ttgcgtttgc aatggcgtac cttcgcggca gatataatgg cggtgcgttt
     1441 acaaaaacag taatcgacgc aacgatgtgc gccattatcg cctggttcat tcgtgacctt
     1501 ctcgacttcg ccggactaag tagcaatctc gcttatataa cgagcgtgtt tatcggctac
     1561 atcggtactg actcgattgg ttcgcttatc aaacgcttcg ctgctaaaaa agccggagta
     1621 gaagatggta gaaatcaata atcaacgtaa ggcgttcctc gatatgctgg cgtggtcgga
     1681 gggaactgat aacggacgtc agaaaaccag aaatcatggt tatgacgtca ttgtaggcgg
     1741 agagctattt actgattact ccgatcaccc tcgcaaactt gtcacgctaa acccaaaact
     1801 caaatcaaca ggcgccggac gctaccagct tctttcccgt tggtgggatg cctaccgcaa
     1861 gcagcttggc ctgaaagact tctctccgaa aagtcaggac gctgtggcat tgcagcagat
     1921 taaggagcgt ggcgctttac ctatgattga tcgtggtgat atccgtcagg caatcgaccg
     1981 ttgcagcaat atctgggctt cactgccggg cgctggttat ggtcagttcg agcataaggc
     2041 tgacagcctg attgcaaaat tcaaagaagc gggcggaacg gtcagagaga ttgatgtatg
     2101 agcagagtca ccgcgattat ctccgctctg gttatctgca tcatcgtctg cctgtcatgg
     2161 gctgttaatc attaccgtga taacgccatt acctacaaag cccagcgcga caaaaatgcc
     2221 agagaactga agctggcgaa cgcggcaatt actgacatgc agatgcgtca gcgtgatgtt
     2281 gctgcgctcg atgcaaaata cacgaaggag ttagctgatg ctaaagctga aaatgatgct
     2341 ctgcgtgatg atgttgccgc tggtcgtcgt cggttgcaca tcaaagcagt ctgtcagtca
     2401 gtgcgtgaag ccaccaccgc ctccggcgtg gataatgcag cctccccccg actggcagac
     2461 accgctgaac gggattattt caccctcaga gagaggctga tcactatgca aaaacaactg
     2521 gaaggaaccc agaagtatat taatgagcag tgcagataga gttactagag ccaggcatca
     2581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2641 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//