LOCUS BBa_K359000 4208 bp DNA linear 22-Nov-2024 DEFINITION AIP sensor and generator ACCESSION BBa_K359000 VERSION BBa_K359000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23107 RBS 44..55 /label=B0034-sequence RBS RBS 1316..1327 /label=B0034-sequence RBS misc_feature 1310..1315 /label=BamHI site CDS 62..1309 /label=agrC coding sequence CDS 1334..1963 /label=agrA coding sequence misc_feature 44..1963 /label=BBa_I746100 stem_loop 1983..2026 /label=stem_loop misc_feature 1972..2051 /label=BBa_B0010 terminator 1972..2051 /label=BBa_B0010 stem_loop 2067..2086 /label=T7 TE misc_feature 2093..2093 /label=stop polyA_site 2087..2100 /label=polya misc_feature 2060..2100 /label=BBa_B0012 terminator 2060..2100 /label=BBa_B0012 promoter 2109..2143 /label=BBa_J23107 RBS 2152..2163 /label=B0034-sequence RBS RBS 3424..3435 /label=B0034-sequence RBS misc_feature 3418..3423 /label=BamHI site CDS 2170..3417 /label=agrC coding sequence CDS 3442..4071 /label=agrA coding sequence misc_feature 2152..4071 /label=BBa_I746100 stem_loop 4091..4134 /label=stem_loop misc_feature 4080..4159 /label=BBa_B0010 terminator 4080..4159 /label=BBa_B0010 stem_loop 4175..4194 /label=T7 TE misc_feature 4201..4201 /label=stop polyA_site 4195..4208 /label=polya misc_feature 4168..4208 /label=BBa_B0012 terminator 4168..4208 /label=BBa_B0012 ORIGIN 1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgattctg atgttcacca ttccagcaat tattagcggt attaagtaca gcaagctgga 121 ttatttcttc atcatcgtaa tctctaccct gtccctgttc ctgttcaaaa tgttcgattc 181 cgcgagcctg attatcctga cttccttcat catcatcatg tatttcgtaa aaatcaaatg 241 gtatagcatc ctgctgatca tgacctccca gattatcctg tactgtgcca actacatgta 301 tatcgttatc tacgcgtaca tcacgaaaat ctccgattct atcttcgtta tctttccgtc 361 tttcttcgtg gtgtacgtta cgatctctat tctgttttcc tatattatca atcgcgttct 421 gaaaaagatc tctactccgt acctgattct gaacaaaggt tttctgatcg tcatctccac 481 tatcctgctg ctgaccttct ctctgttctt cttttactct cagatcaact ccgatgaggc 541 aaaagtcatc cgtcagtaca gcttcatctt tatcggtatt actatcttcc tgagcattct 601 gactttcgtt atcagccagt tcctgctgaa agaaatgaag tacaagcgca accaagaaga 661 aatcgagact tactacgagt acaccctgaa gattgaagcg atcaacaacg aaatgcgtaa 721 atttcgtcac gactacgtta acattctgac caccctgagc gaatacatcc gtgaagacga 781 catgcctggc ctgcgtgatt atttcaacaa aaacattgtg ccgatgaaag ataacctgca 841 aatgaacgct attaagctga acggtatcga aaacctgaaa gtacgtgaga tcaagggtct 901 gattaccgct aaaatcctgc gtgcgcagga aatgaacatc ccaattagca tcgaaatccc 961 agacgaagtt tctagcatta acctgaacat gattgacctg tctcgtagca tcggcatcat 1021 cctggacaac gctatcgaag cttccactga aatcgacgac ccgattattc gcgttgcttt 1081 tatcgagagc gaaaactccg tgacctttat cgtgatgaac aaatgcgcgg acgatatccc 1141 gcgtattcac gaactgttcc aggaatcttt cagcaccaag ggtgaaggtc gcggcctggg 1201 cctgtccacc ctgaaagaga tcgcggataa cgccgacaac gttctgctgg acactattat 1261 cgaaaacggt ttcttcattc agaaagtcga aatcatcaat aactaataag gatccaaaga 1321 ggagaaatac tagatggaaa tcgcactggc tacggacaat ccatatgagg ttctggaaca 1381 ggctaaaaac atgaacgaca tcggctgcta tttcctggat attcagctga gcaccgacat 1441 caacggtatc aaactgggct ctgaaatccg taaacatgat ccggttggta acattatttt 1501 cgtgacttct cattctgagc tgacctatct gacgttcgta tacaaggttg cagccatgga 1561 tttcatcttt aaagacgatc cggcagagct gcgtacgcgc atcatcgact gtctggaaac 1621 tgcgcacacg cgcctgcaac tgctgtccaa agataactcc gtggaaacca ttgaactgaa 1681 acgtggcagc aacagcgttt acgttcagta cgatgatatc atgttcttcg aaagctccac 1741 taagtcccac cgtctgatcg cgcatctgga taatcgtcag atcgagttct atggcaacct 1801 gaaagagctg agccagctgg acgatcgctt cttccgttgt cataactcct tcgttgttaa 1861 ccgtcacaac atcgaatcca tcgactccaa agagcgtatt gtatacttca agaacaaaga 1921 acactgttat gcgtctgtac gcaatgtcaa gaaaatctaa taatactaga gccaggcatc 1981 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2041 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 2101 tactagagtt tacggctagc tcagccctag gtattatgct agctactaga gaaagaggag 2161 aaatactaga tgattctgat gttcaccatt ccagcaatta ttagcggtat taagtacagc 2221 aagctggatt atttcttcat catcgtaatc tctaccctgt ccctgttcct gttcaaaatg 2281 ttcgattccg cgagcctgat tatcctgact tccttcatca tcatcatgta tttcgtaaaa 2341 atcaaatggt atagcatcct gctgatcatg acctcccaga ttatcctgta ctgtgccaac 2401 tacatgtata tcgttatcta cgcgtacatc acgaaaatct ccgattctat cttcgttatc 2461 tttccgtctt tcttcgtggt gtacgttacg atctctattc tgttttccta tattatcaat 2521 cgcgttctga aaaagatctc tactccgtac ctgattctga acaaaggttt tctgatcgtc 2581 atctccacta tcctgctgct gaccttctct ctgttcttct tttactctca gatcaactcc 2641 gatgaggcaa aagtcatccg tcagtacagc ttcatcttta tcggtattac tatcttcctg 2701 agcattctga ctttcgttat cagccagttc ctgctgaaag aaatgaagta caagcgcaac 2761 caagaagaaa tcgagactta ctacgagtac accctgaaga ttgaagcgat caacaacgaa 2821 atgcgtaaat ttcgtcacga ctacgttaac attctgacca ccctgagcga atacatccgt 2881 gaagacgaca tgcctggcct gcgtgattat ttcaacaaaa acattgtgcc gatgaaagat 2941 aacctgcaaa tgaacgctat taagctgaac ggtatcgaaa acctgaaagt acgtgagatc 3001 aagggtctga ttaccgctaa aatcctgcgt gcgcaggaaa tgaacatccc aattagcatc 3061 gaaatcccag acgaagtttc tagcattaac ctgaacatga ttgacctgtc tcgtagcatc 3121 ggcatcatcc tggacaacgc tatcgaagct tccactgaaa tcgacgaccc gattattcgc 3181 gttgctttta tcgagagcga aaactccgtg acctttatcg tgatgaacaa atgcgcggac 3241 gatatcccgc gtattcacga actgttccag gaatctttca gcaccaaggg tgaaggtcgc 3301 ggcctgggcc tgtccaccct gaaagagatc gcggataacg ccgacaacgt tctgctggac 3361 actattatcg aaaacggttt cttcattcag aaagtcgaaa tcatcaataa ctaataagga 3421 tccaaagagg agaaatacta gatggaaatc gcactggcta cggacaatcc atatgaggtt 3481 ctggaacagg ctaaaaacat gaacgacatc ggctgctatt tcctggatat tcagctgagc 3541 accgacatca acggtatcaa actgggctct gaaatccgta aacatgatcc ggttggtaac 3601 attattttcg tgacttctca ttctgagctg acctatctga cgttcgtata caaggttgca 3661 gccatggatt tcatctttaa agacgatccg gcagagctgc gtacgcgcat catcgactgt 3721 ctggaaactg cgcacacgcg cctgcaactg ctgtccaaag ataactccgt ggaaaccatt 3781 gaactgaaac gtggcagcaa cagcgtttac gttcagtacg atgatatcat gttcttcgaa 3841 agctccacta agtcccaccg tctgatcgcg catctggata atcgtcagat cgagttctat 3901 ggcaacctga aagagctgag ccagctggac gatcgcttct tccgttgtca taactccttc 3961 gttgttaacc gtcacaacat cgaatccatc gactccaaag agcgtattgt atacttcaag 4021 aacaaagaac actgttatgc gtctgtacgc aatgtcaaga aaatctaata atactagagc 4081 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 4141 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 4201 cgtttata //