LOCUS BBa_K359002 7246 bp DNA linear 23-Jun-2024 DEFINITION Agr quroum sensing sensor/generator, FepA pore, with P2 + reporter ACCESSION BBa_K359002 VERSION BBa_K359002.1 FEATURES Location/Qualifiers RBS 1..12 /label=B0034-sequence RBS RBS 1273..1284 /label=B0034-sequence RBS misc_feature 1267..1272 /label=BamHI site CDS 19..1266 /label=agrC coding sequence CDS 1291..1920 /label=agrA coding sequence misc_feature 1..1920 /label=BBa_I746100 stem_loop 1940..1983 /label=stem_loop misc_feature 1929..2008 /label=BBa_B0010 terminator 1929..2008 /label=BBa_B0010 stem_loop 2024..2043 /label=T7 TE misc_feature 2050..2050 /label=stop polyA_site 2044..2057 /label=polya misc_feature 2017..2057 /label=BBa_B0012 terminator 2017..2057 /label=BBa_B0012 promoter 2066..2100 /label=BBa_J23107 RBS 2109..2120 /label=B0034-sequence RBS RBS 3381..3392 /label=B0034-sequence RBS misc_feature 3375..3380 /label=BamHI site CDS 2127..3374 /label=agrC coding sequence CDS 3399..4028 /label=agrA coding sequence misc_feature 2109..4028 /label=BBa_I746100 stem_loop 4048..4091 /label=stem_loop misc_feature 4037..4116 /label=BBa_B0010 terminator 4037..4116 /label=BBa_B0010 stem_loop 4132..4151 /label=T7 TE misc_feature 4158..4158 /label=stop polyA_site 4152..4165 /label=polya misc_feature 4125..4165 /label=BBa_B0012 terminator 4125..4165 /label=BBa_B0012 promoter 4174..4208 /label=BBa_J23107 RBS 4217..4228 /label=B0034-sequence RBS RBS 5489..5500 /label=B0034-sequence RBS misc_feature 5483..5488 /label=BamHI site CDS 4235..5482 /label=agrC coding sequence CDS 5507..6136 /label=agrA coding sequence misc_feature 4217..6136 /label=BBa_I746100 stem_loop 6156..6199 /label=stem_loop misc_feature 6145..6224 /label=BBa_B0010 terminator 6145..6224 /label=BBa_B0010 stem_loop 6240..6259 /label=T7 TE misc_feature 6266..6266 /label=stop polyA_site 6260..6273 /label=polya misc_feature 6233..6273 /label=BBa_B0012 terminator 6233..6273 /label=BBa_B0012 misc_feature 1..6273 /label=BBa_K359001 promoter 6282..6377 /label=BBa_I746104 misc_feature 6390..6393 /label=conserved RBS 6386..6397 /label=BBa_B0034 CDS 6404..7078 /label=mrfp1 misc_feature 7085..7109 /label=Help:Barcodes CDS 6404..7084 /label=BBa_E1010 stem_loop 7129..7172 /label=stem_loop misc_feature 7118..7197 /label=BBa_B0010 terminator 7118..7197 /label=BBa_B0010 stem_loop 7213..7232 /label=T7 TE misc_feature 7239..7239 /label=stop polyA_site 7233..7246 /label=polya misc_feature 7206..7246 /label=BBa_B0012 terminator 7206..7246 /label=BBa_B0012 misc_feature 6386..7246 /label=BBa_I13507 misc_feature 6282..7246 /label=BBa_K359003 ORIGIN 1 aaagaggaga aatactagat gattctgatg ttcaccattc cagcaattat tagcggtatt 61 aagtacagca agctggatta tttcttcatc atcgtaatct ctaccctgtc cctgttcctg 121 ttcaaaatgt tcgattccgc gagcctgatt atcctgactt ccttcatcat catcatgtat 181 ttcgtaaaaa tcaaatggta tagcatcctg ctgatcatga cctcccagat tatcctgtac 241 tgtgccaact acatgtatat cgttatctac gcgtacatca cgaaaatctc cgattctatc 301 ttcgttatct ttccgtcttt cttcgtggtg tacgttacga tctctattct gttttcctat 361 attatcaatc gcgttctgaa aaagatctct actccgtacc tgattctgaa caaaggtttt 421 ctgatcgtca tctccactat cctgctgctg accttctctc tgttcttctt ttactctcag 481 atcaactccg atgaggcaaa agtcatccgt cagtacagct tcatctttat cggtattact 541 atcttcctga gcattctgac tttcgttatc agccagttcc tgctgaaaga aatgaagtac 601 aagcgcaacc aagaagaaat cgagacttac tacgagtaca ccctgaagat tgaagcgatc 661 aacaacgaaa tgcgtaaatt tcgtcacgac tacgttaaca ttctgaccac cctgagcgaa 721 tacatccgtg aagacgacat gcctggcctg cgtgattatt tcaacaaaaa cattgtgccg 781 atgaaagata acctgcaaat gaacgctatt aagctgaacg gtatcgaaaa cctgaaagta 841 cgtgagatca agggtctgat taccgctaaa atcctgcgtg cgcaggaaat gaacatccca 901 attagcatcg aaatcccaga cgaagtttct agcattaacc tgaacatgat tgacctgtct 961 cgtagcatcg gcatcatcct ggacaacgct atcgaagctt ccactgaaat cgacgacccg 1021 attattcgcg ttgcttttat cgagagcgaa aactccgtga cctttatcgt gatgaacaaa 1081 tgcgcggacg atatcccgcg tattcacgaa ctgttccagg aatctttcag caccaagggt 1141 gaaggtcgcg gcctgggcct gtccaccctg aaagagatcg cggataacgc cgacaacgtt 1201 ctgctggaca ctattatcga aaacggtttc ttcattcaga aagtcgaaat catcaataac 1261 taataaggat ccaaagagga gaaatactag atggaaatcg cactggctac ggacaatcca 1321 tatgaggttc tggaacaggc taaaaacatg aacgacatcg gctgctattt cctggatatt 1381 cagctgagca ccgacatcaa cggtatcaaa ctgggctctg aaatccgtaa acatgatccg 1441 gttggtaaca ttattttcgt gacttctcat tctgagctga cctatctgac gttcgtatac 1501 aaggttgcag ccatggattt catctttaaa gacgatccgg cagagctgcg tacgcgcatc 1561 atcgactgtc tggaaactgc gcacacgcgc ctgcaactgc tgtccaaaga taactccgtg 1621 gaaaccattg aactgaaacg tggcagcaac agcgtttacg ttcagtacga tgatatcatg 1681 ttcttcgaaa gctccactaa gtcccaccgt ctgatcgcgc atctggataa tcgtcagatc 1741 gagttctatg gcaacctgaa agagctgagc cagctggacg atcgcttctt ccgttgtcat 1801 aactccttcg ttgttaaccg tcacaacatc gaatccatcg actccaaaga gcgtattgta 1861 tacttcaaga acaaagaaca ctgttatgcg tctgtacgca atgtcaagaa aatctaataa 1921 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1981 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2041 gcctttctgc gtttatatac tagagtttac ggctagctca gccctaggta ttatgctagc 2101 tactagagaa agaggagaaa tactagatga ttctgatgtt caccattcca gcaattatta 2161 gcggtattaa gtacagcaag ctggattatt tcttcatcat cgtaatctct accctgtccc 2221 tgttcctgtt caaaatgttc gattccgcga gcctgattat cctgacttcc ttcatcatca 2281 tcatgtattt cgtaaaaatc aaatggtata gcatcctgct gatcatgacc tcccagatta 2341 tcctgtactg tgccaactac atgtatatcg ttatctacgc gtacatcacg aaaatctccg 2401 attctatctt cgttatcttt ccgtctttct tcgtggtgta cgttacgatc tctattctgt 2461 tttcctatat tatcaatcgc gttctgaaaa agatctctac tccgtacctg attctgaaca 2521 aaggttttct gatcgtcatc tccactatcc tgctgctgac cttctctctg ttcttctttt 2581 actctcagat caactccgat gaggcaaaag tcatccgtca gtacagcttc atctttatcg 2641 gtattactat cttcctgagc attctgactt tcgttatcag ccagttcctg ctgaaagaaa 2701 tgaagtacaa gcgcaaccaa gaagaaatcg agacttacta cgagtacacc ctgaagattg 2761 aagcgatcaa caacgaaatg cgtaaatttc gtcacgacta cgttaacatt ctgaccaccc 2821 tgagcgaata catccgtgaa gacgacatgc ctggcctgcg tgattatttc aacaaaaaca 2881 ttgtgccgat gaaagataac ctgcaaatga acgctattaa gctgaacggt atcgaaaacc 2941 tgaaagtacg tgagatcaag ggtctgatta ccgctaaaat cctgcgtgcg caggaaatga 3001 acatcccaat tagcatcgaa atcccagacg aagtttctag cattaacctg aacatgattg 3061 acctgtctcg tagcatcggc atcatcctgg acaacgctat cgaagcttcc actgaaatcg 3121 acgacccgat tattcgcgtt gcttttatcg agagcgaaaa ctccgtgacc tttatcgtga 3181 tgaacaaatg cgcggacgat atcccgcgta ttcacgaact gttccaggaa tctttcagca 3241 ccaagggtga aggtcgcggc ctgggcctgt ccaccctgaa agagatcgcg gataacgccg 3301 acaacgttct gctggacact attatcgaaa acggtttctt cattcagaaa gtcgaaatca 3361 tcaataacta ataaggatcc aaagaggaga aatactagat ggaaatcgca ctggctacgg 3421 acaatccata tgaggttctg gaacaggcta aaaacatgaa cgacatcggc tgctatttcc 3481 tggatattca gctgagcacc gacatcaacg gtatcaaact gggctctgaa atccgtaaac 3541 atgatccggt tggtaacatt attttcgtga cttctcattc tgagctgacc tatctgacgt 3601 tcgtatacaa ggttgcagcc atggatttca tctttaaaga cgatccggca gagctgcgta 3661 cgcgcatcat cgactgtctg gaaactgcgc acacgcgcct gcaactgctg tccaaagata 3721 actccgtgga aaccattgaa ctgaaacgtg gcagcaacag cgtttacgtt cagtacgatg 3781 atatcatgtt cttcgaaagc tccactaagt cccaccgtct gatcgcgcat ctggataatc 3841 gtcagatcga gttctatggc aacctgaaag agctgagcca gctggacgat cgcttcttcc 3901 gttgtcataa ctccttcgtt gttaaccgtc acaacatcga atccatcgac tccaaagagc 3961 gtattgtata cttcaagaac aaagaacact gttatgcgtc tgtacgcaat gtcaagaaaa 4021 tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 4081 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 4141 tcgggtgggc ctttctgcgt ttatatacta gagtttacgg ctagctcagc cctaggtatt 4201 atgctagcta ctagagaaag aggagaaata ctagatgatt ctgatgttca ccattccagc 4261 aattattagc ggtattaagt acagcaagct ggattatttc ttcatcatcg taatctctac 4321 cctgtccctg ttcctgttca aaatgttcga ttccgcgagc ctgattatcc tgacttcctt 4381 catcatcatc atgtatttcg taaaaatcaa atggtatagc atcctgctga tcatgacctc 4441 ccagattatc ctgtactgtg ccaactacat gtatatcgtt atctacgcgt acatcacgaa 4501 aatctccgat tctatcttcg ttatctttcc gtctttcttc gtggtgtacg ttacgatctc 4561 tattctgttt tcctatatta tcaatcgcgt tctgaaaaag atctctactc cgtacctgat 4621 tctgaacaaa ggttttctga tcgtcatctc cactatcctg ctgctgacct tctctctgtt 4681 cttcttttac tctcagatca actccgatga ggcaaaagtc atccgtcagt acagcttcat 4741 ctttatcggt attactatct tcctgagcat tctgactttc gttatcagcc agttcctgct 4801 gaaagaaatg aagtacaagc gcaaccaaga agaaatcgag acttactacg agtacaccct 4861 gaagattgaa gcgatcaaca acgaaatgcg taaatttcgt cacgactacg ttaacattct 4921 gaccaccctg agcgaataca tccgtgaaga cgacatgcct ggcctgcgtg attatttcaa 4981 caaaaacatt gtgccgatga aagataacct gcaaatgaac gctattaagc tgaacggtat 5041 cgaaaacctg aaagtacgtg agatcaaggg tctgattacc gctaaaatcc tgcgtgcgca 5101 ggaaatgaac atcccaatta gcatcgaaat cccagacgaa gtttctagca ttaacctgaa 5161 catgattgac ctgtctcgta gcatcggcat catcctggac aacgctatcg aagcttccac 5221 tgaaatcgac gacccgatta ttcgcgttgc ttttatcgag agcgaaaact ccgtgacctt 5281 tatcgtgatg aacaaatgcg cggacgatat cccgcgtatt cacgaactgt tccaggaatc 5341 tttcagcacc aagggtgaag gtcgcggcct gggcctgtcc accctgaaag agatcgcgga 5401 taacgccgac aacgttctgc tggacactat tatcgaaaac ggtttcttca ttcagaaagt 5461 cgaaatcatc aataactaat aaggatccaa agaggagaaa tactagatgg aaatcgcact 5521 ggctacggac aatccatatg aggttctgga acaggctaaa aacatgaacg acatcggctg 5581 ctatttcctg gatattcagc tgagcaccga catcaacggt atcaaactgg gctctgaaat 5641 ccgtaaacat gatccggttg gtaacattat tttcgtgact tctcattctg agctgaccta 5701 tctgacgttc gtatacaagg ttgcagccat ggatttcatc tttaaagacg atccggcaga 5761 gctgcgtacg cgcatcatcg actgtctgga aactgcgcac acgcgcctgc aactgctgtc 5821 caaagataac tccgtggaaa ccattgaact gaaacgtggc agcaacagcg tttacgttca 5881 gtacgatgat atcatgttct tcgaaagctc cactaagtcc caccgtctga tcgcgcatct 5941 ggataatcgt cagatcgagt tctatggcaa cctgaaagag ctgagccagc tggacgatcg 6001 cttcttccgt tgtcataact ccttcgttgt taaccgtcac aacatcgaat ccatcgactc 6061 caaagagcgt attgtatact tcaagaacaa agaacactgt tatgcgtctg tacgcaatgt 6121 caagaaaatc taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 6181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 6241 gctcaccttc gggtgggcct ttctgcgttt atatactaga gattaaatac aaattacatt 6301 taacagttaa gtatttattt cctacagtta ggcaatataa tgataaaaga ttgtactaaa 6361 tcgtataatg acagtgatac tagagaaaga ggagaaatac tagatggctt cctccgaaga 6421 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga 6481 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact 6541 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca 6601 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc 6661 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac 6721 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg 6781 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc 6841 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa 6901 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc 6961 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga 7021 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta 7081 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag 7141 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 7201 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //