##gff-version 3
##sequence-region BBa_K359002 1 7246
BBa_K359002	.	engineered_region	1	6273	.	+	0	ID=BBa_K359001;Name=BBa_K359001
BBa_K359002	.	terminator	6145	6224	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K359001
BBa_K359002	.	stem_loop	6156	6199	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359002	.	engineered_region	6145	6224	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359002	.	terminator	4125	4165	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K359001
BBa_K359002	.	stem_loop	4132	4151	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359002	.	polyA_site	4152	4165	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359002	.	stop_codon	4158	4158	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359002	.	engineered_region	4125	4165	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359002	.	terminator	6233	6273	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K359001
BBa_K359002	.	stem_loop	6240	6259	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359002	.	polyA_site	6260	6273	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359002	.	stop_codon	6266	6266	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359002	.	engineered_region	6233	6273	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359002	.	engineered_region	1	1920	.	+	0	ID=BBa_I746100;Name=BBa_I746100;Parent=BBa_K359001
BBa_K359002	.	CDS	1291	1920	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K359002	.	CDS	19	1266	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K359002	.	ribosome_entry_site	1	12	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359002	.	sequence_feature	1267	1272	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K359002	.	ribosome_entry_site	1273	1284	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359002	.	engineered_region	4217	6136	.	+	0	ID=BBa_I746100;Name=BBa_I746100;Parent=BBa_K359001
BBa_K359002	.	CDS	5507	6136	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K359002	.	CDS	4235	5482	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K359002	.	ribosome_entry_site	4217	4228	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359002	.	sequence_feature	5483	5488	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K359002	.	ribosome_entry_site	5489	5500	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359002	.	engineered_region	2109	4028	.	+	0	ID=BBa_I746100;Name=BBa_I746100;Parent=BBa_K359001
BBa_K359002	.	CDS	3399	4028	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K359002	.	CDS	2127	3374	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K359002	.	ribosome_entry_site	2109	2120	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359002	.	sequence_feature	3375	3380	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K359002	.	ribosome_entry_site	3381	3392	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359002	.	promoter	2066	2100	.	+	0	ID=BBa_J23107;Name=BBa_J23107;Parent=BBa_K359001
BBa_K359002	.	terminator	4037	4116	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K359001
BBa_K359002	.	stem_loop	4048	4091	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359002	.	engineered_region	4037	4116	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359002	.	terminator	2017	2057	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K359001
BBa_K359002	.	stem_loop	2024	2043	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359002	.	polyA_site	2044	2057	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359002	.	stop_codon	2050	2050	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359002	.	engineered_region	2017	2057	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359002	.	terminator	1929	2008	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K359001
BBa_K359002	.	stem_loop	1940	1983	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359002	.	engineered_region	1929	2008	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359002	.	promoter	4174	4208	.	+	0	ID=BBa_J23107;Name=BBa_J23107;Parent=BBa_K359001
BBa_K359002	.	engineered_region	6282	7246	.	+	0	ID=BBa_K359003;Name=BBa_K359003
BBa_K359002	.	promoter	6282	6377	.	+	0	ID=BBa_I746104;Name=BBa_I746104;Parent=BBa_K359003
BBa_K359002	.	engineered_region	6386	7246	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K359003
BBa_K359002	.	ribosome_entry_site	6386	6397	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K359002	.	conserved_region	6390	6393	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K359002	.	CDS	6404	7084	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K359002	.	CDS	6404	7078	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K359002	.	engineered_tag	7085	7109	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K359002	.	terminator	7206	7246	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K359002	.	stem_loop	7213	7232	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359002	.	polyA_site	7233	7246	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359002	.	stop_codon	7239	7239	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359002	.	engineered_region	7206	7246	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359002	.	terminator	7118	7197	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K359002	.	stem_loop	7129	7172	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359002	.	engineered_region	7118	7197	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K359002
aaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggatt
atttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgac
ttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctg
tactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgt
ctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactcc
gtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttac
tctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagca
ttctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttacta
cgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccacc
ctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagata
acctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaa
aatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatg
attgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattc
gcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattca
cgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggat
aacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaat
aaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaa
aaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaa
atccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtataca
aggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgc
gcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttac
gttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcaga
tcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaa
ccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgta
cgcaatgtcaagaaaatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgattc
tgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctac
cctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttc
gtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcg
ttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgat
ctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggtttt
ctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaa
aagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagtt
cctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcg
atcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacg
acatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagct
gaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaac
atcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggca
tcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaa
ctccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagc
accaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggaca
ctattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatacta
gatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctat
ttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggta
acattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctt
taaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtcc
aaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttct
tcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaaga
gctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgac
tccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctc
agccctaggtattatgctagctactagagaaagaggagaaatactagatgattctgatgttcaccattccagcaattat
tagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatg
ttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcc
tgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaat
ctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatc
aatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgc
tgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcat
ctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaag
cgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttc
gtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattattt
caacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagta
cgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccag
acgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagc
ttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaac
aaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgg
gcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcat
tcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggac
aatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccg
acatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattc
tgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgt
acgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattg
aactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccg
tctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttc
ttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttca
agaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagattaaatacaaattacatttaacagttaagtatttattt
cctacagttaggcaatataatgataaaagattgtactaaatcgtataatgacagtgatactagagaaagaggagaaata
ctagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcac
gagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtg
gtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctga
catcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgtt
gttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgt
ccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctct
gaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggct
aaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacacca
tcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgct
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata