##gff-version 3
##sequence-region BBa_K359005 1 5181
BBa_K359005	.	terminator	1972	2051	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K359005	.	stem_loop	1983	2026	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359005	.	engineered_region	1972	2051	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359005	.	ribosome_entry_site	4321	4332	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K359005	.	conserved_region	4325	4328	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K359005	.	engineered_region	44	1963	.	+	0	ID=BBa_I746100;Name=BBa_I746100
BBa_K359005	.	sequence_feature	1310	1315	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K359005	.	CDS	62	1309	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K359005	.	CDS	1334	1963	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K359005	.	ribosome_entry_site	44	55	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359005	.	ribosome_entry_site	1316	1327	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359005	.	terminator	2060	2100	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K359005	.	stem_loop	2067	2086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359005	.	stop_codon	2093	2093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359005	.	polyA_site	2087	2100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359005	.	engineered_region	2060	2100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359005	.	CDS	4339	5019	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K359005	.	engineered_tag	5020	5044	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K359005	.	CDS	4339	5013	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K359005	.	terminator	4080	4159	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K359005	.	stem_loop	4091	4134	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359005	.	engineered_region	4080	4159	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359005	.	terminator	5053	5132	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K359005	.	stem_loop	5064	5107	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359005	.	engineered_region	5053	5132	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359005	.	promoter	2109	2143	.	+	0	ID=BBa_J23107;Name=BBa_J23107
BBa_K359005	.	promoter	4217	4312	.	+	0	ID=BBa_I746104;Name=BBa_I746104
BBa_K359005	.	engineered_region	2152	4071	.	+	0	ID=BBa_I746100;Name=BBa_I746100
BBa_K359005	.	sequence_feature	3418	3423	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K359005	.	CDS	2170	3417	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K359005	.	CDS	3442	4071	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K359005	.	ribosome_entry_site	2152	2163	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359005	.	ribosome_entry_site	3424	3435	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359005	.	promoter	1	35	.	+	0	ID=BBa_J23107;Name=BBa_J23107
BBa_K359005	.	terminator	4168	4208	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K359005	.	stem_loop	4175	4194	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359005	.	stop_codon	4201	4201	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359005	.	polyA_site	4195	4208	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359005	.	engineered_region	4168	4208	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359005	.	terminator	5141	5181	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K359005	.	stem_loop	5148	5167	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359005	.	stop_codon	5174	5174	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359005	.	polyA_site	5168	5181	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359005	.	engineered_region	5141	5181	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K359005
tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgattctgatgttcacc
attccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgt
tcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaa
atggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcg
tacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgt
tttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcat
ctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgt
cagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaag
aaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacga
aatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggc
ctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcg
aaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattag
catcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggac
aacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacct
ttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtga
aggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaa
aacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcg
cactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatat
tcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttc
gtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatc
cggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactc
cgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctcc
actaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagc
tggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcg
tattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagccctaggta
ttatgctagctactagagaaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtatta
agtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgc
gagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatg
acctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattcta
tcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttct
gaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttc
tctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggta
ttactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaaga
agaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactac
gttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaaca
ttgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaa
gggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttct
agcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaa
tcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcgga
cgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccacc
ctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcg
aaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatga
ggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggt
atcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacct
atctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcat
cgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgt
ggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgc
atctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtca
taactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaa
cactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagattaaatacaaattacatttaacagttaagtatttatttcctacagttag
gcaatataatgataaaagattgtactaaatcgtataatgacagtgatactagagaaagaggagaaatactagatggctt
cctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaat
cgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccg
ttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggact
acctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttac
ccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccg
gttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaa
tcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggt
tcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacag
tacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata