BBa_K359005
type: ComponentDefinition
persistent identity: BBa K359005
display id: BBa_K359005
version: 1
was generated by: iGEM to SBOL conversion
description: Senses AIP and has RFP reporter (from Agr quorum sensing system)
created: 2010-08-17T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: N/A
owned by: myers
owned by: james
mutable description: N/A
discontinued: true
dominant: false
experience: experience/None
group u list: _470_
m user id: 0
owner id: 4002
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: N/A
star: false
creator: Leah Kocsis
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_B0012
component: BBa_B0010
component: BBa_J23107
component: BBa_I746100
component: BBa_I746104
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_J23107
sequence annotation: BBa_I746100
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746104
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K359005_sequence/1

BBa_B0012
type: Component
persistent identity: component2078317
display id: component2078317
version: 1
top level: BBa_K359005
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2078315
display id: component2078315
version: 1
top level: BBa_K359005
definition: BBa_B0010
access: public

BBa_J23107
type: Component
persistent identity: component2078296
display id: component2078296
version: 1
top level: BBa_K359005
definition: BBa_J23107
access: public

BBa_B0012
type: Component
persistent identity: component2078292
display id: component2078292
version: 1
top level: BBa_K359005
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2078303
display id: component2078303
version: 1
top level: BBa_K359005
definition: BBa_B0010
access: public

BBa_I746100
type: Component
persistent identity: component2078302
display id: component2078302
version: 1
top level: BBa_K359005
definition: BBa_I746100
access: public

BBa_I746104
type: Component
persistent identity: component2078309
display id: component2078309
version: 1
top level: BBa_K359005
definition: BBa_I746104
access: public

BBa_J23107
type: Component
persistent identity: component2078283
display id: component2078283
version: 1
top level: BBa_K359005
definition: BBa_J23107
access: public

BBa_B0012
type: Component
persistent identity: component2078305
display id: component2078305
version: 1
top level: BBa_K359005
definition: BBa_B0012
access: public

BBa_E1010
type: Component
persistent identity: component2078313
display id: component2078313
version: 1
top level: BBa_K359005
definition: mRFP1
access: public

BBa_B0010
type: Component
persistent identity: component2078290
display id: component2078290
version: 1
top level: BBa_K359005
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component2078311
display id: component2078311
version: 1
top level: BBa_K359005
definition: BBa_B0034
access: public

BBa_I746100
type: Component
persistent identity: component2078289
display id: component2078289
version: 1
top level: BBa_K359005
definition: BBa_I746100
access: public

BBa_J23107
type: SequenceAnnotation
persistent identity: annotation2078283
display id: annotation2078283
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078283/range2078283/1
component: BBa_J23107

https://synbiohub.org/public/igem/BBa_K359005/annotation2078283/range2078283/1
type: Range
persistent identity: range2078283
display id: range2078283
version: 1
top level: BBa_K359005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I746100
type: SequenceAnnotation
persistent identity: annotation2078289
display id: annotation2078289
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078289/range2078289/1
component: BBa_I746100

https://synbiohub.org/public/igem/BBa_K359005/annotation2078289/range2078289/1
type: Range
persistent identity: range2078289
display id: range2078289
version: 1
top level: BBa_K359005
direction: direction/reverse
start: 44
end: 1963
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078290
display id: annotation2078290
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078290/range2078290/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K359005/annotation2078290/range2078290/1
type: Range
persistent identity: range2078290
display id: range2078290
version: 1
top level: BBa_K359005
direction: direction/forward
start: 1972
end: 2051
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2078292
display id: annotation2078292
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078292/range2078292/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K359005/annotation2078292/range2078292/1
type: Range
persistent identity: range2078292
display id: range2078292
version: 1
top level: BBa_K359005
direction: direction/forward
start: 2060
end: 2100
orientation: inline

BBa_J23107
type: SequenceAnnotation
persistent identity: annotation2078296
display id: annotation2078296
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078296/range2078296/1
component: BBa_J23107

https://synbiohub.org/public/igem/BBa_K359005/annotation2078296/range2078296/1
type: Range
persistent identity: range2078296
display id: range2078296
version: 1
top level: BBa_K359005
direction: direction/reverse
start: 2109
end: 2143
orientation: inline

BBa_I746100
type: SequenceAnnotation
persistent identity: annotation2078302
display id: annotation2078302
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078302/range2078302/1
component: BBa_I746100

https://synbiohub.org/public/igem/BBa_K359005/annotation2078302/range2078302/1
type: Range
persistent identity: range2078302
display id: range2078302
version: 1
top level: BBa_K359005
direction: direction/reverse
start: 2152
end: 4071
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078303
display id: annotation2078303
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078303/range2078303/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K359005/annotation2078303/range2078303/1
type: Range
persistent identity: range2078303
display id: range2078303
version: 1
top level: BBa_K359005
direction: direction/forward
start: 4080
end: 4159
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2078305
display id: annotation2078305
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078305/range2078305/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K359005/annotation2078305/range2078305/1
type: Range
persistent identity: range2078305
display id: range2078305
version: 1
top level: BBa_K359005
direction: direction/forward
start: 4168
end: 4208
orientation: inline

BBa_I746104
type: SequenceAnnotation
persistent identity: annotation2078309
display id: annotation2078309
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078309/range2078309/1
component: BBa_I746104

https://synbiohub.org/public/igem/BBa_K359005/annotation2078309/range2078309/1
type: Range
persistent identity: range2078309
display id: range2078309
version: 1
top level: BBa_K359005
direction: direction/reverse
start: 4217
end: 4312
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2078311
display id: annotation2078311
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078311/range2078311/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K359005/annotation2078311/range2078311/1
type: Range
persistent identity: range2078311
display id: range2078311
version: 1
top level: BBa_K359005
direction: direction/reverse
start: 4321
end: 4332
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2078313
display id: annotation2078313
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078313/range2078313/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K359005/annotation2078313/range2078313/1
type: Range
persistent identity: range2078313
display id: range2078313
version: 1
top level: BBa_K359005
direction: direction/reverse
start: 4339
end: 5019
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2078315
display id: annotation2078315
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078315/range2078315/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K359005/annotation2078315/range2078315/1
type: Range
persistent identity: range2078315
display id: range2078315
version: 1
top level: BBa_K359005
direction: direction/forward
start: 5053
end: 5132
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2078317
display id: annotation2078317
version: 1
top level: BBa_K359005
location: https://synbiohub.org/public/igem/BBa_K359005/annotation2078317/range2078317/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K359005/annotation2078317/range2078317/1
type: Range
persistent identity: range2078317
display id: range2078317
version: 1
top level: BBa_K359005
direction: direction/forward
start: 5141
end: 5181
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I746100
type: ComponentDefinition
persistent identity: BBa I746100
display id: BBa_I746100
version: 1
was generated by: iGEM to SBOL conversion
description: AIP sensor infrastructure (agrC + agrA from S. aureus with RBSes)
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The original sequences for agrC and agrA were taken from the ''S. aureus'' Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for ''E. coli'' using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
mutable description: This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrC and agrA (in that order) from ''S. aureus'' strain NCTC8325, prefixed by the B0034 RBS and separated by a BamHI site.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 1851
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: David Wyatt
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: B0034-sequence RBS
sequence annotation: agrC coding sequence
sequence annotation: BamHI site
sequence annotation: agrA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I746100_sequence/1

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1951374
display id: annotation1951374
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951374/range1951374/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I746100/annotation1951374/range1951374/1
type: Range
persistent identity: range1951374
display id: range1951374
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1
end: 12
orientation: inline

agrC coding sequence
type: SequenceAnnotation
persistent identity: annotation1951377
display id: annotation1951377
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951377/range1951377/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746100/annotation1951377/range1951377/1
type: Range
persistent identity: range1951377
display id: range1951377
version: 1
top level: BBa_I746100
direction: direction/forward
start: 19
end: 1266
orientation: inline

BamHI site
type: SequenceAnnotation
persistent identity: annotation1951376
display id: annotation1951376
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951376/range1951376/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I746100/annotation1951376/range1951376/1
type: Range
persistent identity: range1951376
display id: range1951376
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1267
end: 1272
orientation: inline

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1951375
display id: annotation1951375
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951375/range1951375/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I746100/annotation1951375/range1951375/1
type: Range
persistent identity: range1951375
display id: range1951375
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1273
end: 1284
orientation: inline

agrA coding sequence
type: SequenceAnnotation
persistent identity: annotation1951378
display id: annotation1951378
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951378/range1951378/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746100/annotation1951378/range1951378/1
type: Range
persistent identity: range1951378
display id: range1951378
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1291
end: 1920
orientation: inline

BBa_I746104
type: ComponentDefinition
persistent identity: BBa I746104
display id: BBa_I746104
version: 1
was generated by: iGEM to SBOL conversion
description: P2 promoter in agr operon from S. aureus
created: 2007-08-30T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
star: true
creator: Zhizhen Zhao
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746104_sequence/1

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K359005_sequence/1
type: Sequence
persistent identity: BBa K359005 sequence
display id: BBa_K359005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccaggcatcaaataaaacgaaaggctcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746100_sequence/1
type: Sequence
persistent identity: BBa I746100 sequence
display id: BBa_I746100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746104_sequence/1
type: Sequence
persistent identity: BBa I746104 sequence
display id: BBa_I746104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaatacaaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataatgacagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23107_sequence/1
type: Sequence
persistent identity: BBa J23107 sequence
display id: BBa_J23107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagccctaggtattatgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z