LOCUS       BBa_K359006             3047 bp    DNA     linear       26-Nov-2024
DEFINITION  AIP sensor and generator
ACCESSION   BBa_K359006
VERSION     BBa_K359006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23107
     RBS             44..55
                     /label=B0034-sequence RBS
     RBS             1316..1327
                     /label=B0034-sequence RBS
     misc_feature    1310..1315
                     /label=BamHI site
     CDS             62..1309
                     /label=agrC coding sequence
     CDS             1334..1963
                     /label=agrA coding sequence
     misc_feature    44..1963
                     /label=BBa_I746100
     stem_loop       1983..2026
                     /label=stem_loop
     misc_feature    1972..2051
                     /label=BBa_B0010
     terminator      1972..2051
                     /label=BBa_B0010
     stem_loop       2067..2086
                     /label=T7 TE
     misc_feature    2093..2093
                     /label=stop
     polyA_site      2087..2100
                     /label=polya
     misc_feature    2060..2100
                     /label=BBa_B0012
     terminator      2060..2100
                     /label=BBa_B0012
     promoter        2109..2143
                     /label=BBa_J23107
     RBS             2152..2163
                     /label=B0034-sequence RBS
     CDS             2170..2742
                     /label=agrB coding sequence
     RBS             2749..2760
                     /label=B0034-sequence RBS
     misc_feature    2743..2748
                     /label=SaII site
     CDS             2767..2910
                     /label=agrD coding sequence
     misc_feature    2152..2910
                     /label=BBa_I746000
     stem_loop       2930..2973
                     /label=stem_loop
     misc_feature    2919..2998
                     /label=BBa_B0010
     terminator      2919..2998
                     /label=BBa_B0010
     stem_loop       3014..3033
                     /label=T7 TE
     misc_feature    3040..3040
                     /label=stop
     polyA_site      3034..3047
                     /label=polya
     misc_feature    3007..3047
                     /label=BBa_B0012
     terminator      3007..3047
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgattctg atgttcacca ttccagcaat tattagcggt attaagtaca gcaagctgga
      121 ttatttcttc atcatcgtaa tctctaccct gtccctgttc ctgttcaaaa tgttcgattc
      181 cgcgagcctg attatcctga cttccttcat catcatcatg tatttcgtaa aaatcaaatg
      241 gtatagcatc ctgctgatca tgacctccca gattatcctg tactgtgcca actacatgta
      301 tatcgttatc tacgcgtaca tcacgaaaat ctccgattct atcttcgtta tctttccgtc
      361 tttcttcgtg gtgtacgtta cgatctctat tctgttttcc tatattatca atcgcgttct
      421 gaaaaagatc tctactccgt acctgattct gaacaaaggt tttctgatcg tcatctccac
      481 tatcctgctg ctgaccttct ctctgttctt cttttactct cagatcaact ccgatgaggc
      541 aaaagtcatc cgtcagtaca gcttcatctt tatcggtatt actatcttcc tgagcattct
      601 gactttcgtt atcagccagt tcctgctgaa agaaatgaag tacaagcgca accaagaaga
      661 aatcgagact tactacgagt acaccctgaa gattgaagcg atcaacaacg aaatgcgtaa
      721 atttcgtcac gactacgtta acattctgac caccctgagc gaatacatcc gtgaagacga
      781 catgcctggc ctgcgtgatt atttcaacaa aaacattgtg ccgatgaaag ataacctgca
      841 aatgaacgct attaagctga acggtatcga aaacctgaaa gtacgtgaga tcaagggtct
      901 gattaccgct aaaatcctgc gtgcgcagga aatgaacatc ccaattagca tcgaaatccc
      961 agacgaagtt tctagcatta acctgaacat gattgacctg tctcgtagca tcggcatcat
     1021 cctggacaac gctatcgaag cttccactga aatcgacgac ccgattattc gcgttgcttt
     1081 tatcgagagc gaaaactccg tgacctttat cgtgatgaac aaatgcgcgg acgatatccc
     1141 gcgtattcac gaactgttcc aggaatcttt cagcaccaag ggtgaaggtc gcggcctggg
     1201 cctgtccacc ctgaaagaga tcgcggataa cgccgacaac gttctgctgg acactattat
     1261 cgaaaacggt ttcttcattc agaaagtcga aatcatcaat aactaataag gatccaaaga
     1321 ggagaaatac tagatggaaa tcgcactggc tacggacaat ccatatgagg ttctggaaca
     1381 ggctaaaaac atgaacgaca tcggctgcta tttcctggat attcagctga gcaccgacat
     1441 caacggtatc aaactgggct ctgaaatccg taaacatgat ccggttggta acattatttt
     1501 cgtgacttct cattctgagc tgacctatct gacgttcgta tacaaggttg cagccatgga
     1561 tttcatcttt aaagacgatc cggcagagct gcgtacgcgc atcatcgact gtctggaaac
     1621 tgcgcacacg cgcctgcaac tgctgtccaa agataactcc gtggaaacca ttgaactgaa
     1681 acgtggcagc aacagcgttt acgttcagta cgatgatatc atgttcttcg aaagctccac
     1741 taagtcccac cgtctgatcg cgcatctgga taatcgtcag atcgagttct atggcaacct
     1801 gaaagagctg agccagctgg acgatcgctt cttccgttgt cataactcct tcgttgttaa
     1861 ccgtcacaac atcgaatcca tcgactccaa agagcgtatt gtatacttca agaacaaaga
     1921 acactgttat gcgtctgtac gcaatgtcaa gaaaatctaa taatactaga gccaggcatc
     1981 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2041 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     2101 tactagagtt tacggctagc tcagccctag gtattatgct agctactaga gaaagaggag
     2161 aaatactaga tgaactattt tgacaacaag atcgaccaat ttgctactta cctgcaaaaa
     2221 cgcaacaacc tggaccacat ccagtttctg caagttcgtc tgggcatgca ggttctggct
     2281 aaaaacatcg gcaaactgat cgttatgtac accatcgcct atattctgaa catcttcctg
     2341 tttaccctga tcaccaacct gaccttctat ctgattcgcc gtcatgccca cggcgctcat
     2401 gcaccttctt ccttttggtg ttatgtagag agcattattc tgtttatcct gctgccgctg
     2461 gtaatcgtta acttccacat caatttcctg attatgatca tcctgactgt gatttccctg
     2521 ggtgtaatct ctgtttacgc tccggctgcc actaagaaaa agccgattcc ggtgcgcctg
     2581 atcaaacgta aaaagtacta cgctatcatc gtttccctga ccctgttcat tatcaccctg
     2641 attatcaaag aaccgtttgc acagttcatc cagctgggta ttatcatcga agcgatcact
     2701 ctgctgccga tctttttcat caaagaggat ctgaaataat aagtcgacaa agaggagaaa
     2761 tactagatga atactctgtt caatctgttt ttcgatttca ttactggcat cctgaagaac
     2821 atcggtaaca tcgcagcgta cagcacctgc gacttcatca tggatgaagt agaggtcccg
     2881 aaagagctga cccagctgca tgaataataa tactagagcc aggcatcaaa taaaacgaaa
     2941 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     3001 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//