LOCUS BBa_K359007 4514 bp DNA linear 25-Nov-2024 DEFINITION AIP sensor with FepA pore ACCESSION BBa_K359007 VERSION BBa_K359007.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23107 RBS 44..55 /label=B0034-sequence RBS RBS 1316..1327 /label=B0034-sequence RBS misc_feature 1310..1315 /label=BamHI site CDS 62..1309 /label=agrC coding sequence CDS 1334..1963 /label=agrA coding sequence misc_feature 44..1963 /label=BBa_I746100 stem_loop 1983..2026 /label=stem_loop misc_feature 1972..2051 /label=BBa_B0010 terminator 1972..2051 /label=BBa_B0010 stem_loop 2067..2086 /label=T7 TE misc_feature 2093..2093 /label=stop polyA_site 2087..2100 /label=polya misc_feature 2060..2100 /label=BBa_B0012 terminator 2060..2100 /label=BBa_B0012 promoter 2109..2143 /label=BBa_J23111 misc_feature 2156..2159 /label=conserved RBS 2152..2163 /label=BBa_B0034 CDS 2170..4377 /label=BBa_I746200 stem_loop 4397..4440 /label=stem_loop misc_feature 4386..4465 /label=BBa_B0010 terminator 4386..4465 /label=BBa_B0010 stem_loop 4481..4500 /label=T7 TE misc_feature 4507..4507 /label=stop polyA_site 4501..4514 /label=polya misc_feature 4474..4514 /label=BBa_B0012 terminator 4474..4514 /label=BBa_B0012 ORIGIN 1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgattctg atgttcacca ttccagcaat tattagcggt attaagtaca gcaagctgga 121 ttatttcttc atcatcgtaa tctctaccct gtccctgttc ctgttcaaaa tgttcgattc 181 cgcgagcctg attatcctga cttccttcat catcatcatg tatttcgtaa aaatcaaatg 241 gtatagcatc ctgctgatca tgacctccca gattatcctg tactgtgcca actacatgta 301 tatcgttatc tacgcgtaca tcacgaaaat ctccgattct atcttcgtta tctttccgtc 361 tttcttcgtg gtgtacgtta cgatctctat tctgttttcc tatattatca atcgcgttct 421 gaaaaagatc tctactccgt acctgattct gaacaaaggt tttctgatcg tcatctccac 481 tatcctgctg ctgaccttct ctctgttctt cttttactct cagatcaact ccgatgaggc 541 aaaagtcatc cgtcagtaca gcttcatctt tatcggtatt actatcttcc tgagcattct 601 gactttcgtt atcagccagt tcctgctgaa agaaatgaag tacaagcgca accaagaaga 661 aatcgagact tactacgagt acaccctgaa gattgaagcg atcaacaacg aaatgcgtaa 721 atttcgtcac gactacgtta acattctgac caccctgagc gaatacatcc gtgaagacga 781 catgcctggc ctgcgtgatt atttcaacaa aaacattgtg ccgatgaaag ataacctgca 841 aatgaacgct attaagctga acggtatcga aaacctgaaa gtacgtgaga tcaagggtct 901 gattaccgct aaaatcctgc gtgcgcagga aatgaacatc ccaattagca tcgaaatccc 961 agacgaagtt tctagcatta acctgaacat gattgacctg tctcgtagca tcggcatcat 1021 cctggacaac gctatcgaag cttccactga aatcgacgac ccgattattc gcgttgcttt 1081 tatcgagagc gaaaactccg tgacctttat cgtgatgaac aaatgcgcgg acgatatccc 1141 gcgtattcac gaactgttcc aggaatcttt cagcaccaag ggtgaaggtc gcggcctggg 1201 cctgtccacc ctgaaagaga tcgcggataa cgccgacaac gttctgctgg acactattat 1261 cgaaaacggt ttcttcattc agaaagtcga aatcatcaat aactaataag gatccaaaga 1321 ggagaaatac tagatggaaa tcgcactggc tacggacaat ccatatgagg ttctggaaca 1381 ggctaaaaac atgaacgaca tcggctgcta tttcctggat attcagctga gcaccgacat 1441 caacggtatc aaactgggct ctgaaatccg taaacatgat ccggttggta acattatttt 1501 cgtgacttct cattctgagc tgacctatct gacgttcgta tacaaggttg cagccatgga 1561 tttcatcttt aaagacgatc cggcagagct gcgtacgcgc atcatcgact gtctggaaac 1621 tgcgcacacg cgcctgcaac tgctgtccaa agataactcc gtggaaacca ttgaactgaa 1681 acgtggcagc aacagcgttt acgttcagta cgatgatatc atgttcttcg aaagctccac 1741 taagtcccac cgtctgatcg cgcatctgga taatcgtcag atcgagttct atggcaacct 1801 gaaagagctg agccagctgg acgatcgctt cttccgttgt cataactcct tcgttgttaa 1861 ccgtcacaac atcgaatcca tcgactccaa agagcgtatt gtatacttca agaacaaaga 1921 acactgttat gcgtctgtac gcaatgtcaa gaaaatctaa taatactaga gccaggcatc 1981 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2041 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 2101 tactagagtt gacggctagc tcagtcctag gtatagtgct agctactaga gaaagaggag 2161 aaatactaga tgaacaagaa gattcattcc ctggccttgt tggtcaatct ggggatttat 2221 ggggtagcgc aggcacaaga gccgaccgat actcctgttt cacatgacga tactattgtc 2281 gttaccgccg ccgagcagaa cttacaggcg cctggcgttt cgaccatcac cgcagatgaa 2341 atccgcaaaa acccggttgc ccgcgatgtg tcgaagatca tccgtaccat gccaggcgtt 2401 aacctgaccg gtaactccac cagtggtcag cgtgggaata accgacagat tgatattcgc 2461 ggtatgggtc cggaaaacac gctgattttg attgacggca agccggtaag cagccgtaac 2521 tcggtgcgtc agggctggcg tggcgagcgc gatacccgtg gtgatacttc ctgggtgcca 2581 cctgaaatga ttgaacgtat tgaagttctg cgtggtccgg cagctgcgcg ttatggcaac 2641 ggcgcggcgg gcggcgtggt taacatcatt accaaaaaag gcagcggcga gtggcacggc 2701 tcctgggacg catatttcaa tgcgccagaa cataaagagg aaggtgccac caaacgcact 2761 aactttagcc tgaccggtcc gctgggcgac gagttcagct tccgtttgta tggcaacctc 2821 gacaaaaccc aggctgacgc gtgggatatc aaccagggcc atcagtccgc gcgtgccgga 2881 acgtatgcca cgacgttacc agccgggcgc gaaggggtaa tcaacaaaga tattaatggc 2941 gtggtgcgct gggatttcgc gccattgcaa tcgctggaac tggaagcagg ttacagccgc 3001 cagggtaacc tgtatgcggg cgacacccag aataccaact ccgattccta tacccgctcg 3061 aaatatggcg atgaaaccaa ccgtctgtat cgccagaact acgcgctgac ctggaacggt 3121 ggctgggata acggcgtgac caccagcaac tgggtgcagt acgaacacac ccgtaactcg 3181 cgtattccgg aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa agcgacacag 3241 gatttcgtcg atatcgatct tgatgacgtg atgctgcaca gcgaagttaa cctgccgatt 3301 gatttcctcg ttaaccagac gctgacgctg ggtacggagt ggaatcagca acggatgaag 3361 gacttaagtt ccaacaccca ggcactgacc ggaacgaata ccggtggcgc tattgatggc 3421 gtgagtacca ccgaccgtag cccgtattca aaagcagaaa ttttctcgct gtttgccgaa 3481 aacaacatgg agctgactga cagcaccatc gtaacgccgg ggctgcgttt cgatcatcac 3541 agtattgtcg gcaataactg gagcccggcg ctgaacatat cgcaaggttt aggcgatgac 3601 ttcacgctga aaatgggcat cgcccgtgct tataaagcgc cgagcctgta ccagactaac 3661 ccgaactaca ttctctacag taaaggtcag ggttgctatg ccagcgcggg cggctgctat 3721 ctgcaaggta acgatgacct gaaagcagaa accagcatca acaaagagat tggtctggag 3781 ttcaaacgcg acgggtggct ggcgggcgtc acctggttcc gtaacgatta tcgcaataag 3841 attgaagcgg gctatgtggc tgtagggcaa aattgtgaaa cagcggtggt tgaaggtctg 3901 gaaggatcgt taaacgtacc ggttagcgaa acggtgatgt ggaccaataa catcacttat 3961 atgctgaaga gtgaaaacaa aaccacgggc gaccgtttgt cgatcatccc ggagtatacg 4021 ttgaactcaa cgctgagctg gcaggcacgg gaagatttgt cgatgcaaac gaccttcacc 4081 tggtacggca agcagcagcc gaagaagtac aactataaag gtcagccagc ggttggaccg 4141 gaaaccaaag aaattagtcc ttacagcatt gttggcctga gcgcgacctg ggatgtgacg 4201 aagaatgtca gtctgaccgg cggcgtggac aatctgttcg acaaacgttt gtggcgtgcg 4261 ggtaatgccc agaccacggg cgatttggca ggggccaact atatcgccgg tgccggggcg 4321 tatacctata acgagccggg acgtacgtgg tatatgagcg taaacaccca cttctgatac 4381 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 4441 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 4501 tttctgcgtt tata //