BBa_K359007
type: ComponentDefinition
persistent identity: BBa K359007
display id: BBa_K359007
version: 1
was generated by: iGEM to SBOL conversion
description: AIP sensor with FepA pore
created: 2010-09-01T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: This part includes the S.aureus Agr quorum sensing sensor which recognizes the oligopeptide. The FepA pore allows for the oligopeptide to diffuse through the outer-membrane.
discontinued: false
dominant: false
group u list: _470_
m user id: 0
owner id: 7330
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: George Zarubin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J23107
component: BBa_B0010
component: BBa_I746100
component: BBa_J23111
component: BBa_B0012
component: BBa_I746200
component: BBa_B0034
sequence annotation: BBa_J23107
sequence annotation: BBa_I746100
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23111
sequence annotation: BBa_B0034
sequence annotation: BBa_I746200
sequence: https://synbiohub.org/public/igem/BBa_K359007_sequence/1

BBa_J23107
type: Component
persistent identity: component2079424
display id: component2079424
version: 1
top level: BBa_K359007
definition: BBa_J23107
access: public

BBa_B0010
type: Component
persistent identity: component2079431
display id: component2079431
version: 1
top level: BBa_K359007
definition: BBa_B0010
access: public

BBa_I746100
type: Component
persistent identity: component2079430
display id: component2079430
version: 1
top level: BBa_K359007
definition: BBa_I746100
access: public

BBa_J23111
type: Component
persistent identity: component2079437
display id: component2079437
version: 1
top level: BBa_K359007
definition: BBa_J23111
access: public

BBa_B0012
type: Component
persistent identity: component2079433
display id: component2079433
version: 1
top level: BBa_K359007
definition: BBa_B0012
access: public

BBa_I746200
type: Component
persistent identity: component2079440
display id: component2079440
version: 1
top level: BBa_K359007
definition: BBa_I746200
access: public

BBa_B0034
type: Component
persistent identity: component2079439
display id: component2079439
version: 1
top level: BBa_K359007
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2079443
display id: component2079443
version: 1
top level: BBa_K359007
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2079441
display id: component2079441
version: 1
top level: BBa_K359007
definition: BBa_B0010
access: public

BBa_J23107
type: SequenceAnnotation
persistent identity: annotation2079424
display id: annotation2079424
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079424/range2079424/1
component: BBa_J23107

https://synbiohub.org/public/igem/BBa_K359007/annotation2079424/range2079424/1
type: Range
persistent identity: range2079424
display id: range2079424
version: 1
top level: BBa_K359007
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I746100
type: SequenceAnnotation
persistent identity: annotation2079430
display id: annotation2079430
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079430/range2079430/1
component: BBa_I746100

https://synbiohub.org/public/igem/BBa_K359007/annotation2079430/range2079430/1
type: Range
persistent identity: range2079430
display id: range2079430
version: 1
top level: BBa_K359007
direction: direction/reverse
start: 44
end: 1963
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2079431
display id: annotation2079431
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079431/range2079431/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K359007/annotation2079431/range2079431/1
type: Range
persistent identity: range2079431
display id: range2079431
version: 1
top level: BBa_K359007
direction: direction/forward
start: 1972
end: 2051
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2079433
display id: annotation2079433
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079433/range2079433/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K359007/annotation2079433/range2079433/1
type: Range
persistent identity: range2079433
display id: range2079433
version: 1
top level: BBa_K359007
direction: direction/forward
start: 2060
end: 2100
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation2079437
display id: annotation2079437
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079437/range2079437/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_K359007/annotation2079437/range2079437/1
type: Range
persistent identity: range2079437
display id: range2079437
version: 1
top level: BBa_K359007
direction: direction/reverse
start: 2109
end: 2143
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2079439
display id: annotation2079439
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079439/range2079439/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K359007/annotation2079439/range2079439/1
type: Range
persistent identity: range2079439
display id: range2079439
version: 1
top level: BBa_K359007
direction: direction/reverse
start: 2152
end: 2163
orientation: inline

BBa_I746200
type: SequenceAnnotation
persistent identity: annotation2079440
display id: annotation2079440
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079440/range2079440/1
component: BBa_I746200

https://synbiohub.org/public/igem/BBa_K359007/annotation2079440/range2079440/1
type: Range
persistent identity: range2079440
display id: range2079440
version: 1
top level: BBa_K359007
direction: direction/reverse
start: 2170
end: 4377
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2079441
display id: annotation2079441
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079441/range2079441/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K359007/annotation2079441/range2079441/1
type: Range
persistent identity: range2079441
display id: range2079441
version: 1
top level: BBa_K359007
direction: direction/forward
start: 4386
end: 4465
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2079443
display id: annotation2079443
version: 1
top level: BBa_K359007
location: https://synbiohub.org/public/igem/BBa_K359007/annotation2079443/range2079443/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K359007/annotation2079443/range2079443/1
type: Range
persistent identity: range2079443
display id: range2079443
version: 1
top level: BBa_K359007
direction: direction/forward
start: 4474
end: 4514
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I746100
type: ComponentDefinition
persistent identity: BBa I746100
display id: BBa_I746100
version: 1
was generated by: iGEM to SBOL conversion
description: AIP sensor infrastructure (agrC + agrA from S. aureus with RBSes)
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The original sequences for agrC and agrA were taken from the ''S. aureus'' Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for ''E. coli'' using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
mutable description: This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrC and agrA (in that order) from ''S. aureus'' strain NCTC8325, prefixed by the B0034 RBS and separated by a BamHI site.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 1851
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: David Wyatt
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: B0034-sequence RBS
sequence annotation: agrC coding sequence
sequence annotation: BamHI site
sequence annotation: agrA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I746100_sequence/1

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1951374
display id: annotation1951374
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951374/range1951374/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I746100/annotation1951374/range1951374/1
type: Range
persistent identity: range1951374
display id: range1951374
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1
end: 12
orientation: inline

agrC coding sequence
type: SequenceAnnotation
persistent identity: annotation1951377
display id: annotation1951377
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951377/range1951377/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746100/annotation1951377/range1951377/1
type: Range
persistent identity: range1951377
display id: range1951377
version: 1
top level: BBa_I746100
direction: direction/forward
start: 19
end: 1266
orientation: inline

BamHI site
type: SequenceAnnotation
persistent identity: annotation1951376
display id: annotation1951376
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951376/range1951376/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I746100/annotation1951376/range1951376/1
type: Range
persistent identity: range1951376
display id: range1951376
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1267
end: 1272
orientation: inline

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1951375
display id: annotation1951375
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951375/range1951375/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I746100/annotation1951375/range1951375/1
type: Range
persistent identity: range1951375
display id: range1951375
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1273
end: 1284
orientation: inline

agrA coding sequence
type: SequenceAnnotation
persistent identity: annotation1951378
display id: annotation1951378
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951378/range1951378/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746100/annotation1951378/range1951378/1
type: Range
persistent identity: range1951378
display id: range1951378
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1291
end: 1920
orientation: inline

BBa_I746200
type: ComponentDefinition
persistent identity: BBa I746200
display id: BBa_I746200
version: 1
was generated by: iGEM to SBOL conversion
description: FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2118
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.
star: true
creator: Narin Hengrung
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I746200_sequence/1

BBa_J23111
type: ComponentDefinition
persistent identity: BBa J23111
display id: BBa_J23111
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23111_sequence/1

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

https://synbiohub.org/public/igem/BBa_K359007_sequence/1
type: Sequence
persistent identity: BBa K359007 sequence
display id: BBa_K359007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccaggcatcaaataaaacgaaaggctcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746100_sequence/1
type: Sequence
persistent identity: BBa I746100 sequence
display id: BBa_I746100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746200_sequence/1
type: Sequence
persistent identity: BBa I746200 sequence
display id: BBa_I746200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacaagaagattcattccctggccttgttggtcaatctggggatttatggggtagcgcaggcacaagagccgaccgatactcctgtttcacatgacgatactattgtcgttaccgccgccgagcagaacttacaggcgcctggcgtttcgaccatcaccgcagatgaaatccgcaaaaacccggttgcccgcgatgtgtcgaagatcatccgtaccatgccaggcgttaacctgaccggtaactccaccagtggtcagcgtgggaataaccgacagattgatattcgcggtatgggtccggaaaacacgctgattttgattgacggcaagccggtaagcagccgtaactcggtgcgtcagggctggcgtggcgagcgcgatacccgtggtgatacttcctgggtgccacctgaaatgattgaacgtattgaagttctgcgtggtccggcagctgcgcgttatggcaacggcgcggcgggcggcgtggttaacatcattaccaaaaaaggcagcggcgagtggcacggctcctgggacgcatatttcaatgcgccagaacataaagaggaaggtgccaccaaacgcactaactttagcctgaccggtccgctgggcgacgagttcagcttccgtttgtatggcaacctcgacaaaacccaggctgacgcgtgggatatcaaccagggccatcagtccgcgcgtgccggaacgtatgccacgacgttaccagccgggcgcgaaggggtaatcaacaaagatattaatggcgtggtgcgctgggatttcgcgccattgcaatcgctggaactggaagcaggttacagccgccagggtaacctgtatgcgggcgacacccagaataccaactccgattcctatacccgctcgaaatatggcgatgaaaccaaccgtctgtatcgccagaactacgcgctgacctggaacggtggctgggataacggcgtgaccaccagcaactgggtgcagtacgaacacacccgtaactcgcgtattccggaaggtctggcgggcggtaccgaagggaaatttaacgaaaaagcgacacaggatttcgtcgatatcgatcttgatgacgtgatgctgcacagcgaagttaacctgccgattgatttcctcgttaaccagacgctgacgctgggtacggagtggaatcagcaacggatgaaggacttaagttccaacacccaggcactgaccggaacgaataccggtggcgctattgatggcgtgagtaccaccgaccgtagcccgtattcaaaagcagaaattttctcgctgtttgccgaaaacaacatggagctgactgacagcaccatcgtaacgccggggctgcgtttcgatcatcacagtattgtcggcaataactggagcccggcgctgaacatatcgcaaggtttaggcgatgacttcacgctgaaaatgggcatcgcccgtgcttataaagcgccgagcctgtaccagactaacccgaactacattctctacagtaaaggtcagggttgctatgccagcgcgggcggctgctatctgcaaggtaacgatgacctgaaagcagaaaccagcatcaacaaagagattggtctggagttcaaacgcgacgggtggctggcgggcgtcacctggttccgtaacgattatcgcaataagattgaagcgggctatgtggctgtagggcaaaattgtgaaacagcggtggttgaaggtctggaaggatcgttaaacgtaccggttagcgaaacggtgatgtggaccaataacatcacttatatgctgaagagtgaaaacaaaaccacgggcgaccgtttgtcgatcatcccggagtatacgttgaactcaacgctgagctggcaggcacgggaagatttgtcgatgcaaacgaccttcacctggtacggcaagcagcagccgaagaagtacaactataaaggtcagccagcggttggaccggaaaccaaagaaattagtccttacagcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23111_sequence/1
type: Sequence
persistent identity: BBa J23111 sequence
display id: BBa_J23111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23107_sequence/1
type: Sequence
persistent identity: BBa J23107 sequence
display id: BBa_J23107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagccctaggtattatgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z