LOCUS BBa_K359008 4020 bp DNA linear 16-Jun-2024 DEFINITION AIP generator and sensor, with an added RFP reporter ACCESSION BBa_K359008 VERSION BBa_K359008.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23107 RBS 44..55 /label=B0034-sequence RBS RBS 1316..1327 /label=B0034-sequence RBS misc_feature 1310..1315 /label=BamHI site CDS 62..1309 /label=agrC coding sequence CDS 1334..1963 /label=agrA coding sequence misc_feature 44..1963 /label=BBa_I746100 stem_loop 1983..2026 /label=stem_loop misc_feature 1972..2051 /label=BBa_B0010 terminator 1972..2051 /label=BBa_B0010 stem_loop 2067..2086 /label=T7 TE misc_feature 2093..2093 /label=stop polyA_site 2087..2100 /label=polya misc_feature 2060..2100 /label=BBa_B0012 terminator 2060..2100 /label=BBa_B0012 promoter 2109..2143 /label=BBa_J23107 RBS 2152..2163 /label=B0034-sequence RBS CDS 2170..2742 /label=agrB coding sequence RBS 2749..2760 /label=B0034-sequence RBS misc_feature 2743..2748 /label=SaII site CDS 2767..2910 /label=agrD coding sequence misc_feature 2152..2910 /label=BBa_I746000 stem_loop 2930..2973 /label=stem_loop misc_feature 2919..2998 /label=BBa_B0010 terminator 2919..2998 /label=BBa_B0010 stem_loop 3014..3033 /label=T7 TE misc_feature 3040..3040 /label=stop polyA_site 3034..3047 /label=polya misc_feature 3007..3047 /label=BBa_B0012 terminator 3007..3047 /label=BBa_B0012 misc_feature 1..3047 /label=BBa_K359006 promoter 3056..3151 /label=BBa_I746104 misc_feature 3164..3167 /label=conserved RBS 3160..3171 /label=BBa_B0034 CDS 3178..3852 /label=mrfp1 misc_feature 3859..3883 /label=Help:Barcodes CDS 3178..3858 /label=BBa_E1010 stem_loop 3903..3946 /label=stem_loop misc_feature 3892..3971 /label=BBa_B0010 terminator 3892..3971 /label=BBa_B0010 stem_loop 3987..4006 /label=T7 TE misc_feature 4013..4013 /label=stop polyA_site 4007..4020 /label=polya misc_feature 3980..4020 /label=BBa_B0012 terminator 3980..4020 /label=BBa_B0012 misc_feature 3160..4020 /label=BBa_I13507 misc_feature 3056..4020 /label=BBa_K359003 ORIGIN 1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgattctg atgttcacca ttccagcaat tattagcggt attaagtaca gcaagctgga 121 ttatttcttc atcatcgtaa tctctaccct gtccctgttc ctgttcaaaa tgttcgattc 181 cgcgagcctg attatcctga cttccttcat catcatcatg tatttcgtaa aaatcaaatg 241 gtatagcatc ctgctgatca tgacctccca gattatcctg tactgtgcca actacatgta 301 tatcgttatc tacgcgtaca tcacgaaaat ctccgattct atcttcgtta tctttccgtc 361 tttcttcgtg gtgtacgtta cgatctctat tctgttttcc tatattatca atcgcgttct 421 gaaaaagatc tctactccgt acctgattct gaacaaaggt tttctgatcg tcatctccac 481 tatcctgctg ctgaccttct ctctgttctt cttttactct cagatcaact ccgatgaggc 541 aaaagtcatc cgtcagtaca gcttcatctt tatcggtatt actatcttcc tgagcattct 601 gactttcgtt atcagccagt tcctgctgaa agaaatgaag tacaagcgca accaagaaga 661 aatcgagact tactacgagt acaccctgaa gattgaagcg atcaacaacg aaatgcgtaa 721 atttcgtcac gactacgtta acattctgac caccctgagc gaatacatcc gtgaagacga 781 catgcctggc ctgcgtgatt atttcaacaa aaacattgtg ccgatgaaag ataacctgca 841 aatgaacgct attaagctga acggtatcga aaacctgaaa gtacgtgaga tcaagggtct 901 gattaccgct aaaatcctgc gtgcgcagga aatgaacatc ccaattagca tcgaaatccc 961 agacgaagtt tctagcatta acctgaacat gattgacctg tctcgtagca tcggcatcat 1021 cctggacaac gctatcgaag cttccactga aatcgacgac ccgattattc gcgttgcttt 1081 tatcgagagc gaaaactccg tgacctttat cgtgatgaac aaatgcgcgg acgatatccc 1141 gcgtattcac gaactgttcc aggaatcttt cagcaccaag ggtgaaggtc gcggcctggg 1201 cctgtccacc ctgaaagaga tcgcggataa cgccgacaac gttctgctgg acactattat 1261 cgaaaacggt ttcttcattc agaaagtcga aatcatcaat aactaataag gatccaaaga 1321 ggagaaatac tagatggaaa tcgcactggc tacggacaat ccatatgagg ttctggaaca 1381 ggctaaaaac atgaacgaca tcggctgcta tttcctggat attcagctga gcaccgacat 1441 caacggtatc aaactgggct ctgaaatccg taaacatgat ccggttggta acattatttt 1501 cgtgacttct cattctgagc tgacctatct gacgttcgta tacaaggttg cagccatgga 1561 tttcatcttt aaagacgatc cggcagagct gcgtacgcgc atcatcgact gtctggaaac 1621 tgcgcacacg cgcctgcaac tgctgtccaa agataactcc gtggaaacca ttgaactgaa 1681 acgtggcagc aacagcgttt acgttcagta cgatgatatc atgttcttcg aaagctccac 1741 taagtcccac cgtctgatcg cgcatctgga taatcgtcag atcgagttct atggcaacct 1801 gaaagagctg agccagctgg acgatcgctt cttccgttgt cataactcct tcgttgttaa 1861 ccgtcacaac atcgaatcca tcgactccaa agagcgtatt gtatacttca agaacaaaga 1921 acactgttat gcgtctgtac gcaatgtcaa gaaaatctaa taatactaga gccaggcatc 1981 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2041 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 2101 tactagagtt tacggctagc tcagccctag gtattatgct agctactaga gaaagaggag 2161 aaatactaga tgaactattt tgacaacaag atcgaccaat ttgctactta cctgcaaaaa 2221 cgcaacaacc tggaccacat ccagtttctg caagttcgtc tgggcatgca ggttctggct 2281 aaaaacatcg gcaaactgat cgttatgtac accatcgcct atattctgaa catcttcctg 2341 tttaccctga tcaccaacct gaccttctat ctgattcgcc gtcatgccca cggcgctcat 2401 gcaccttctt ccttttggtg ttatgtagag agcattattc tgtttatcct gctgccgctg 2461 gtaatcgtta acttccacat caatttcctg attatgatca tcctgactgt gatttccctg 2521 ggtgtaatct ctgtttacgc tccggctgcc actaagaaaa agccgattcc ggtgcgcctg 2581 atcaaacgta aaaagtacta cgctatcatc gtttccctga ccctgttcat tatcaccctg 2641 attatcaaag aaccgtttgc acagttcatc cagctgggta ttatcatcga agcgatcact 2701 ctgctgccga tctttttcat caaagaggat ctgaaataat aagtcgacaa agaggagaaa 2761 tactagatga atactctgtt caatctgttt ttcgatttca ttactggcat cctgaagaac 2821 atcggtaaca tcgcagcgta cagcacctgc gacttcatca tggatgaagt agaggtcccg 2881 aaagagctga cccagctgca tgaataataa tactagagcc aggcatcaaa taaaacgaaa 2941 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3001 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagattaa 3061 atacaaatta catttaacag ttaagtattt atttcctaca gttaggcaat ataatgataa 3121 aagattgtac taaatcgtat aatgacagtg atactagaga aagaggagaa atactagatg 3181 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc 3241 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc 3301 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg 3361 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac 3421 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac 3481 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa 3541 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg 3601 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc 3661 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac 3721 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc 3781 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac 3841 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc 3901 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3961 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //