##gff-version 3
##sequence-region BBa_K359008 1 4020
BBa_K359008	.	engineered_region	1	3047	.	+	0	ID=BBa_K359006;Name=BBa_K359006
BBa_K359008	.	promoter	1	35	.	+	0	ID=BBa_J23107;Name=BBa_J23107;Parent=BBa_K359006
BBa_K359008	.	engineered_region	2152	2910	.	+	0	ID=BBa_I746000;Name=BBa_I746000;Parent=BBa_K359006
BBa_K359008	.	CDS	2170	2742	.	+	0	ID=annotation1950179;Name=agrB coding sequence;Parent=BBa_I746000
BBa_K359008	.	ribosome_entry_site	2749	2760	.	+	0	ID=annotation1950180;Name=B0034-sequence RBS;Parent=BBa_I746000
BBa_K359008	.	ribosome_entry_site	2152	2163	.	+	0	ID=annotation1950178;Name=B0034-sequence RBS;Parent=BBa_I746000
BBa_K359008	.	CDS	2767	2910	.	+	0	ID=annotation1950182;Name=agrD coding sequence;Parent=BBa_I746000
BBa_K359008	.	sequence_feature	2743	2748	.	+	0	ID=annotation1950181;Name=SaII site;Parent=BBa_I746000
BBa_K359008	.	engineered_region	44	1963	.	+	0	ID=BBa_I746100;Name=BBa_I746100;Parent=BBa_K359006
BBa_K359008	.	CDS	1334	1963	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K359008	.	CDS	62	1309	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K359008	.	ribosome_entry_site	44	55	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359008	.	sequence_feature	1310	1315	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K359008	.	ribosome_entry_site	1316	1327	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359008	.	promoter	2109	2143	.	+	0	ID=BBa_J23107;Name=BBa_J23107;Parent=BBa_K359006
BBa_K359008	.	terminator	2919	2998	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K359006
BBa_K359008	.	stem_loop	2930	2973	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359008	.	engineered_region	2919	2998	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359008	.	terminator	1972	2051	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K359006
BBa_K359008	.	stem_loop	1983	2026	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359008	.	engineered_region	1972	2051	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359008	.	terminator	2060	2100	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K359006
BBa_K359008	.	stem_loop	2067	2086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359008	.	polyA_site	2087	2100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359008	.	stop_codon	2093	2093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359008	.	engineered_region	2060	2100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359008	.	terminator	3007	3047	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K359006
BBa_K359008	.	stem_loop	3014	3033	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359008	.	polyA_site	3034	3047	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359008	.	stop_codon	3040	3040	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359008	.	engineered_region	3007	3047	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359008	.	engineered_region	3056	4020	.	+	0	ID=BBa_K359003;Name=BBa_K359003
BBa_K359008	.	promoter	3056	3151	.	+	0	ID=BBa_I746104;Name=BBa_I746104;Parent=BBa_K359003
BBa_K359008	.	engineered_region	3160	4020	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K359003
BBa_K359008	.	ribosome_entry_site	3160	3171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K359008	.	conserved_region	3164	3167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K359008	.	CDS	3178	3858	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K359008	.	CDS	3178	3852	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K359008	.	engineered_tag	3859	3883	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K359008	.	terminator	3980	4020	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K359008	.	stem_loop	3987	4006	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359008	.	polyA_site	4007	4020	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359008	.	stop_codon	4013	4013	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359008	.	engineered_region	3980	4020	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359008	.	terminator	3892	3971	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K359008	.	stem_loop	3903	3946	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359008	.	engineered_region	3892	3971	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K359008
tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgattctgatgttcacc
attccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgt
tcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaa
atggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcg
tacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgt
tttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcat
ctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgt
cagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaag
aaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacga
aatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggc
ctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcg
aaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattag
catcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggac
aacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacct
ttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtga
aggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaa
aacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcg
cactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatat
tcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttc
gtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatc
cggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactc
cgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctcc
actaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagc
tggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcg
tattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagccctaggta
ttatgctagctactagagaaagaggagaaatactagatgaactattttgacaacaagatcgaccaatttgctacttacc
tgcaaaaacgcaacaacctggaccacatccagtttctgcaagttcgtctgggcatgcaggttctggctaaaaacatcgg
caaactgatcgttatgtacaccatcgcctatattctgaacatcttcctgtttaccctgatcaccaacctgaccttctat
ctgattcgccgtcatgcccacggcgctcatgcaccttcttccttttggtgttatgtagagagcattattctgtttatcc
tgctgccgctggtaatcgttaacttccacatcaatttcctgattatgatcatcctgactgtgatttccctgggtgtaat
ctctgtttacgctccggctgccactaagaaaaagccgattccggtgcgcctgatcaaacgtaaaaagtactacgctatc
atcgtttccctgaccctgttcattatcaccctgattatcaaagaaccgtttgcacagttcatccagctgggtattatca
tcgaagcgatcactctgctgccgatctttttcatcaaagaggatctgaaataataagtcgacaaagaggagaaatacta
gatgaatactctgttcaatctgtttttcgatttcattactggcatcctgaagaacatcggtaacatcgcagcgtacagc
acctgcgacttcatcatggatgaagtagaggtcccgaaagagctgacccagctgcatgaataataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatacaaattacatttaacagt
taagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataatgacagtgatactagaga
aagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttc
cgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaa
gttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtta
aacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcga
agacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggt
accaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccgg
aagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaac
cacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaac
gaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgct
agtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata