##gff-version 3
##sequence-region BBa_K359009 1 5487
BBa_K359009	.	engineered_region	1	4514	.	+	0	ID=BBa_K359007;Name=BBa_K359007
BBa_K359009	.	ribosome_entry_site	2152	2163	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K359007
BBa_K359009	.	conserved_region	2156	2159	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K359009	.	promoter	1	35	.	+	0	ID=BBa_J23107;Name=BBa_J23107;Parent=BBa_K359007
BBa_K359009	.	terminator	2060	2100	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K359007
BBa_K359009	.	engineered_region	2060	2100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359009	.	stop_codon	2093	2093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359009	.	polyA_site	2087	2100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359009	.	stem_loop	2067	2086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359009	.	terminator	4386	4465	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K359007
BBa_K359009	.	engineered_region	4386	4465	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359009	.	stem_loop	4397	4440	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359009	.	terminator	4474	4514	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K359007
BBa_K359009	.	engineered_region	4474	4514	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359009	.	stop_codon	4507	4507	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359009	.	polyA_site	4501	4514	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359009	.	stem_loop	4481	4500	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359009	.	promoter	2109	2143	.	+	0	ID=BBa_J23111;Name=BBa_J23111;Parent=BBa_K359007
BBa_K359009	.	engineered_region	44	1963	.	+	0	ID=BBa_I746100;Name=BBa_I746100;Parent=BBa_K359007
BBa_K359009	.	ribosome_entry_site	44	55	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359009	.	ribosome_entry_site	1316	1327	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K359009	.	CDS	1334	1963	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K359009	.	CDS	62	1309	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K359009	.	sequence_feature	1310	1315	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K359009	.	terminator	1972	2051	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K359007
BBa_K359009	.	engineered_region	1972	2051	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359009	.	stem_loop	1983	2026	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K359009	.	CDS	2170	4377	.	+	0	ID=BBa_I746200;Name=BBa_I746200;Parent=BBa_K359007
BBa_K359009	.	engineered_region	4523	5487	.	+	0	ID=BBa_K359003;Name=BBa_K359003
BBa_K359009	.	promoter	4523	4618	.	+	0	ID=BBa_I746104;Name=BBa_I746104;Parent=BBa_K359003
BBa_K359009	.	engineered_region	4627	5487	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K359003
BBa_K359009	.	CDS	4645	5325	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K359009	.	CDS	4645	5319	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K359009	.	engineered_tag	5326	5350	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K359009	.	terminator	5447	5487	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K359009	.	engineered_region	5447	5487	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K359009	.	stop_codon	5480	5480	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K359009	.	polyA_site	5474	5487	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K359009	.	stem_loop	5454	5473	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K359009	.	ribosome_entry_site	4627	4638	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K359009	.	conserved_region	4631	4634	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K359009	.	terminator	5359	5438	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K359009	.	engineered_region	5359	5438	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K359009	.	stem_loop	5370	5413	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K359009
tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgattctgatgttcacc
attccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgt
tcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaa
atggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcg
tacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgt
tttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcat
ctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgt
cagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaag
aaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacga
aatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggc
ctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcg
aaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattag
catcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggac
aacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacct
ttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtga
aggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaa
aacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcg
cactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatat
tcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttc
gtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatc
cggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactc
cgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctcc
actaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagc
tggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcg
tattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggta
tagtgctagctactagagaaagaggagaaatactagatgaacaagaagattcattccctggccttgttggtcaatctgg
ggatttatggggtagcgcaggcacaagagccgaccgatactcctgtttcacatgacgatactattgtcgttaccgccgc
cgagcagaacttacaggcgcctggcgtttcgaccatcaccgcagatgaaatccgcaaaaacccggttgcccgcgatgtg
tcgaagatcatccgtaccatgccaggcgttaacctgaccggtaactccaccagtggtcagcgtgggaataaccgacaga
ttgatattcgcggtatgggtccggaaaacacgctgattttgattgacggcaagccggtaagcagccgtaactcggtgcg
tcagggctggcgtggcgagcgcgatacccgtggtgatacttcctgggtgccacctgaaatgattgaacgtattgaagtt
ctgcgtggtccggcagctgcgcgttatggcaacggcgcggcgggcggcgtggttaacatcattaccaaaaaaggcagcg
gcgagtggcacggctcctgggacgcatatttcaatgcgccagaacataaagaggaaggtgccaccaaacgcactaactt
tagcctgaccggtccgctgggcgacgagttcagcttccgtttgtatggcaacctcgacaaaacccaggctgacgcgtgg
gatatcaaccagggccatcagtccgcgcgtgccggaacgtatgccacgacgttaccagccgggcgcgaaggggtaatca
acaaagatattaatggcgtggtgcgctgggatttcgcgccattgcaatcgctggaactggaagcaggttacagccgcca
gggtaacctgtatgcgggcgacacccagaataccaactccgattcctatacccgctcgaaatatggcgatgaaaccaac
cgtctgtatcgccagaactacgcgctgacctggaacggtggctgggataacggcgtgaccaccagcaactgggtgcagt
acgaacacacccgtaactcgcgtattccggaaggtctggcgggcggtaccgaagggaaatttaacgaaaaagcgacaca
ggatttcgtcgatatcgatcttgatgacgtgatgctgcacagcgaagttaacctgccgattgatttcctcgttaaccag
acgctgacgctgggtacggagtggaatcagcaacggatgaaggacttaagttccaacacccaggcactgaccggaacga
ataccggtggcgctattgatggcgtgagtaccaccgaccgtagcccgtattcaaaagcagaaattttctcgctgtttgc
cgaaaacaacatggagctgactgacagcaccatcgtaacgccggggctgcgtttcgatcatcacagtattgtcggcaat
aactggagcccggcgctgaacatatcgcaaggtttaggcgatgacttcacgctgaaaatgggcatcgcccgtgcttata
aagcgccgagcctgtaccagactaacccgaactacattctctacagtaaaggtcagggttgctatgccagcgcgggcgg
ctgctatctgcaaggtaacgatgacctgaaagcagaaaccagcatcaacaaagagattggtctggagttcaaacgcgac
gggtggctggcgggcgtcacctggttccgtaacgattatcgcaataagattgaagcgggctatgtggctgtagggcaaa
attgtgaaacagcggtggttgaaggtctggaaggatcgttaaacgtaccggttagcgaaacggtgatgtggaccaataa
catcacttatatgctgaagagtgaaaacaaaaccacgggcgaccgtttgtcgatcatcccggagtatacgttgaactca
acgctgagctggcaggcacgggaagatttgtcgatgcaaacgaccttcacctggtacggcaagcagcagccgaagaagt
acaactataaaggtcagccagcggttggaccggaaaccaaagaaattagtccttacagcattgttggcctgagcgcgac
ctgggatgtgacgaagaatgtcagtctgaccggcggcgtggacaatctgttcgacaaacgtttgtggcgtgcgggtaat
gcccagaccacgggcgatttggcaggggccaactatatcgccggtgccggggcgtatacctataacgagccgggacgta
cgtggtatatgagcgtaaacacccacttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagattaaatacaaattacatttaacagttaagtatttatttcctacagttaggcaatataat
gataaaagattgtactaaatcgtataatgacagtgatactagagaaagaggagaaatactagatggcttcctccgaaga
cgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaa
ggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggg
acatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaact
gtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcc
tccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcaga
aaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcg
tctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccg
ggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtg
ctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata