LOCUS       BBa_K360111             1127 bp    DNA     linear       15-Dec-2024
DEFINITION  J23100 + RBS + LRE + BBa_B0015
ACCESSION   BBa_K360111
VERSION     BBa_K360111.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..47
                     /label=RBS
     misc_feature    48..54
                     /label=Spacer
     RBS             44..54
                     /label=BBa_K360031
     CDS             61..990
                     /label=BBa_K360112
     stem_loop       1010..1053
                     /label=stem_loop
     misc_feature    999..1078
                     /label=BBa_B0010
     terminator      999..1078
                     /label=BBa_B0010
     stem_loop       1094..1113
                     /label=T7 TE
     misc_feature    1120..1120
                     /label=stop
     polyA_site      1114..1127
                     /label=polya
     misc_feature    1087..1127
                     /label=BBa_B0012
     terminator      1087..1127
                     /label=BBa_B0012
     terminator      999..1127
                     /label=BBa_B0015
     misc_feature    44..1127
                     /label=BBa_K360113
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaggaaac agcttactag
       61 atggggccag ttgttgaaaa aattgcagaa cttggcaagt atacggttgg agaaggtcct
      121 cactgggatc atgaaactca gaccttatat ttcgtcgaca ccgtagagaa aacttttcat
      181 aaatatgtac cttctcagaa aaaatacacg ttttgtaaag tagataaact ggtttcattc
      241 attattcccc ttgctggatc ccctggccgt tttgtagtca gtttggaacg tgaaatagcc
      301 attctcacgt gggatggcgt tagtgctgca cctacgagca tagaagctat tgttaatgtc
      361 gaaccccaca ttaaaaataa cagactcaat gatggcaaag cagatcctct tggcaatcta
      421 tggacaggta caatggctat tgacgctggt ctccccgtag gaccggtcac tggcagttta
      481 tatcatttag gggctgataa aaaggtaaaa atgcacgaga gcaacatagc tatagcaaat
      541 gggctcgcgt ggagtaatga tttgaagaaa atgtattata ttgattcggg aaaaagaaga
      601 gtagacgagt acgattatga tgcttctaca ttatccatca gcaatcaacg gccattattt
      661 acttttgaaa agcatgaagt gcctggatat ccagatggtc aaacaattga tgcggagggt
      721 aatttatggg ttgccgtttt ccaaggacag cgaattatta aaatcagtac cgaacaaccg
      781 gaagtgttac tggataccgt aaaaatacca gatcctcagg tcacctctgt tgcatttggc
      841 ggtccgaatt tggatgaact gtatgtaaca tctgctggtc ttcagcttga cgacagttct
      901 tttgacaaaa gtttagttaa tgggcacgtc tacagagtaa caggtttagg cgtcaaaggt
      961 ttcgcgggag ttaaagtgaa gctataataa tactagagcc aggcatcaaa taaaacgaaa
     1021 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1081 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//