BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K360112 1 BBa_K360112 Luciferin Regenerating Enzyme (LRE) from Photinus Pyralis 2010-10-13T11:00:00Z 2015-05-08T01:12:12Z Originally extracted from Photinus pyralis by Gomi and Kajiyama and engineered for codon optimization and registry standards. LRE function, as its name describes, consists in regenerating luciferin from the luminescent product oxyluciferin, which is the product of the luciferase reaction. (Gomi, K. and Kajiyama,N. (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin). false false _485_ 0 6649 9 Not in stock false None false Mariana Ruiz Velasco Leyva BBa_K360031 1 BBa_K360031 Strong RBS with spacer. 2010-10-20T11:00:00Z 2015-05-08T01:12:12Z From lacZ gene of Escherichia coli. Strong RBS with a seven bases spacer before start codon. false false _485_ 0 6838 9 Not in stock false It works very well, although the spacer is not the strongest. false Jorge Arturo Zepeda Mart??nez annotation2091558 1 Spacer range2091558 1 5 11 annotation2091557 1 RBS range2091557 1 1 4 BBa_K360111 1 BBa_K360111 J23100 + RBS + LRE + BBa_B0015 2010-10-09T11:00:00Z 2015-05-08T01:12:12Z LRE was first described by Gomi, K. and Kajiyama, N. from the Research and Development Division, Kikkoman Corporation. Luciferin Regenerating Enzyme (LRE) serves to recycle luciferin; thus, in the presence of a strain containing a luciferase encoding gene, the system will need only an initial inoculation to become autonomous. false false _485_ 0 6649 9 It's complicated true Construction is ligated into pSB1C3. false Mariana Ruiz Velasco Leyva component2091581 1 BBa_J23100 component2091593 1 BBa_K360113 annotation2091581 1 BBa_J23100 range2091581 1 1 35 annotation2091593 1 BBa_K360113 range2091593 1 44 1127 BBa_K360113 1 BBa_K360113 RBS + LRE + BBa_B0015 2010-10-13T11:00:00Z 2015-05-08T01:12:12Z LRE was first described by Gomi, K. and Kajiyama, N. from the Research and Development Division, Kikkoman Corporation. See also BBa_K360112 (LRE CDS). Luciferin Regenerating Enzyme (LRE) serves to recycle luciferin; thus, in the presence of a strain containing a luciferase encoding gene, the system will need only an initial inoculation to become autonomous. false false _485_ 0 6649 9 It's complicated true The construction contains an RBS and is ligated into pSB1C3. false Mariana Ruiz Velasco Leyva component2091564 1 BBa_K360031 component2091572 1 BBa_B0015 component2091565 1 BBa_K360112 annotation2091572 1 BBa_B0015 range2091572 1 956 1084 annotation2091564 1 BBa_K360031 range2091564 1 1 11 annotation2091565 1 BBa_K360112 range2091565 1 18 947 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K360112_sequence 1 atggggccagttgttgaaaaaattgcagaacttggcaagtatacggttggagaaggtcctcactgggatcatgaaactcagaccttatatttcgtcgacaccgtagagaaaacttttcataaatatgtaccttctcagaaaaaatacacgttttgtaaagtagataaactggtttcattcattattccccttgctggatcccctggccgttttgtagtcagtttggaacgtgaaatagccattctcacgtgggatggcgttagtgctgcacctacgagcatagaagctattgttaatgtcgaaccccacattaaaaataacagactcaatgatggcaaagcagatcctcttggcaatctatggacaggtacaatggctattgacgctggtctccccgtaggaccggtcactggcagtttatatcatttaggggctgataaaaaggtaaaaatgcacgagagcaacatagctatagcaaatgggctcgcgtggagtaatgatttgaagaaaatgtattatattgattcgggaaaaagaagagtagacgagtacgattatgatgcttctacattatccatcagcaatcaacggccattatttacttttgaaaagcatgaagtgcctggatatccagatggtcaaacaattgatgcggagggtaatttatgggttgccgttttccaaggacagcgaattattaaaatcagtaccgaacaaccggaagtgttactggataccgtaaaaataccagatcctcaggtcacctctgttgcatttggcggtccgaatttggatgaactgtatgtaacatctgctggtcttcagcttgacgacagttcttttgacaaaagtttagttaatgggcacgtctacagagtaacaggtttaggcgtcaaaggtttcgcgggagttaaagtgaagctataataa BBa_K360031_sequence 1 aggaaacagct BBa_K360111_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaggaaacagcttactagatggggccagttgttgaaaaaattgcagaacttggcaagtatacggttggagaaggtcctcactgggatcatgaaactcagaccttatatttcgtcgacaccgtagagaaaacttttcataaatatgtaccttctcagaaaaaatacacgttttgtaaagtagataaactggtttcattcattattccccttgctggatcccctggccgttttgtagtcagtttggaacgtgaaatagccattctcacgtgggatggcgttagtgctgcacctacgagcatagaagctattgttaatgtcgaaccccacattaaaaataacagactcaatgatggcaaagcagatcctcttggcaatctatggacaggtacaatggctattgacgctggtctccccgtaggaccggtcactggcagtttatatcatttaggggctgataaaaaggtaaaaatgcacgagagcaacatagctatagcaaatgggctcgcgtggagtaatgatttgaagaaaatgtattatattgattcgggaaaaagaagagtagacgagtacgattatgatgcttctacattatccatcagcaatcaacggccattatttacttttgaaaagcatgaagtgcctggatatccagatggtcaaacaattgatgcggagggtaatttatgggttgccgttttccaaggacagcgaattattaaaatcagtaccgaacaaccggaagtgttactggataccgtaaaaataccagatcctcaggtcacctctgttgcatttggcggtccgaatttggatgaactgtatgtaacatctgctggtcttcagcttgacgacagttcttttgacaaaagtttagttaatgggcacgtctacagagtaacaggtttaggcgtcaaaggtttcgcgggagttaaagtgaagctataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K360113_sequence 1 aggaaacagcttactagatggggccagttgttgaaaaaattgcagaacttggcaagtatacggttggagaaggtcctcactgggatcatgaaactcagaccttatatttcgtcgacaccgtagagaaaacttttcataaatatgtaccttctcagaaaaaatacacgttttgtaaagtagataaactggtttcattcattattccccttgctggatcccctggccgttttgtagtcagtttggaacgtgaaatagccattctcacgtgggatggcgttagtgctgcacctacgagcatagaagctattgttaatgtcgaaccccacattaaaaataacagactcaatgatggcaaagcagatcctcttggcaatctatggacaggtacaatggctattgacgctggtctccccgtaggaccggtcactggcagtttatatcatttaggggctgataaaaaggtaaaaatgcacgagagcaacatagctatagcaaatgggctcgcgtggagtaatgatttgaagaaaatgtattatattgattcgggaaaaagaagagtagacgagtacgattatgatgcttctacattatccatcagcaatcaacggccattatttacttttgaaaagcatgaagtgcctggatatccagatggtcaaacaattgatgcggagggtaatttatgggttgccgttttccaaggacagcgaattattaaaatcagtaccgaacaaccggaagtgttactggataccgtaaaaataccagatcctcaggtcacctctgttgcatttggcggtccgaatttggatgaactgtatgtaacatctgctggtcttcagcttgacgacagttcttttgacaaaagtttagttaatgggcacgtctacagagtaacaggtttaggcgtcaaaggtttcgcgggagttaaagtgaagctataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z