LOCUS       BBa_K360125             1928 bp    DNA     linear       10-Dec-2025
DEFINITION  trpL promoter + cI inverter + GFP
ACCESSION   BBa_K360125
VERSION     BBa_K360125.1  
FEATURES             Location/Qualifiers
     misc_feature    29..45
                     /label=trpR binding site -3.5
     misc_feature    21..40
                     /label=trpR binding site  -11.5
     misc_feature    13..30
                     /label=trpR binding site  -19.5
     promoter        3..11
                     /label=-35 
     promoter        28..35
                     /label=-10
     promoter        1..49
                     /label=BBa_K360023
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             79..786
                     /label=cI lambda
     misc_feature    787..819
                     /label=LVA
     misc_feature    826..850
                     /label=Help:Barcodes
     CDS             76..825
                     /label=BBa_C0051
     stem_loop       870..913
                     /label=stem_loop
     misc_feature    859..938
                     /label=BBa_B0010
     terminator      859..938
                     /label=BBa_B0010
     stem_loop       954..973
                     /label=T7 TE
     misc_feature    980..980
                     /label=stop
     polyA_site      974..987
                     /label=polya
     misc_feature    947..987
                     /label=BBa_B0012
     terminator      947..987
                     /label=BBa_B0012
     misc_feature    58..987
                     /label=BBa_P0451
     promoter        1033..1038
                     /label=-10
     promoter        1010..1015
                     /label=-35
     misc_feature    1020..1036
                     /label=OR1
     misc_feature    996..1012
                     /label=OR2
     misc_feature    996..1044
                     /label=BBa_R0051
     promoter        996..1044
                     /label=BBa_R0051
     RBS             1053..1065
                     /label=RBS-3\Weak
     RBS             1059..1062
                     /label=RBS
     misc_feature    1053..1065
                     /label=BBa_B0032
     RBS             1053..1065
                     /label=BBa_B0032
     CDS             1072..1791
                     /label=GFP protein
     CDS             1072..1791
                     /label=BBa_E0040
     stem_loop       1811..1854
                     /label=stem_loop
     misc_feature    1800..1879
                     /label=BBa_B0010
     terminator      1800..1879
                     /label=BBa_B0010
     stem_loop       1895..1914
                     /label=T7 TE
     misc_feature    1921..1921
                     /label=stop
     polyA_site      1915..1928
                     /label=polya
     misc_feature    1888..1928
                     /label=BBa_B0012
     terminator      1888..1928
                     /label=BBa_B0012
     misc_feature    996..1928
                     /label=BBa_K098991
     misc_feature    58..1928
                     /label=BBa_K360024
ORIGIN
        1 gctgttgaca attaatcatc gaactagtta actagtacgc aagttcacgt actagagaaa
       61 gaggagaaat actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac
      121 gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa
      181 tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc
      241 aatgcattaa atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa
      301 gaatttagcc cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag
      361 ccgtcactta gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc
      421 tcacctgagc ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa
      481 aaagccagtg attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca
      541 ggctccaagc caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt
      601 gagccaggtg atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg
      661 atcagggata gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca
      721 tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg
      781 tttggcgctg caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag
      841 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      961 cttcgggtgg gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt tgactatttt
     1021 acctctggcg gtgataatgg ttgctactag agtcacacag gaaagtacta gatgcgtaaa
     1081 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     1141 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     1201 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     1261 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
     1321 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     1381 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     1441 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     1501 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     1561 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1621 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1681 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1741 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
     1801 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1861 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1921 cgtttata
//