BBa_K360031 1 BBa_K360031 Strong RBS with spacer. 2010-10-20T11:00:00Z 2015-05-08T01:12:12Z From lacZ gene of Escherichia coli. Strong RBS with a seven bases spacer before start codon. false false _485_ 0 6838 9 Not in stock false It works very well, although the spacer is not the strongest. false Jorge Arturo Zepeda Mart??nez annotation2091557 1 RBS range2091557 1 1 4 annotation2091558 1 Spacer range2091558 1 5 11 BBa_K360143 1 BBa_K360143 J23101 promoter + Strong RBS + GFP E0040 2010-10-21T11:00:00Z 2015-05-08T01:12:12Z Promoter [[Part:BBa_J23101|BBa_J23101]] and GFP [[Part:BBa_E0040|BBa_E0040]] were taken from the registry. Strong RBS [[Part:BBa_K360031|BBa_K360031]] was taken from lacZ gene of Escherichia coli This part is a composite of the promoter [[Part:BBa_J23101|BBa_J23101]] with our Strong RBS [[Part:BBa_K360031|BBa_K360031]] and the GFP [[Part:BBa_E0040|BBa_E0040]]. This part should express GFP constitutively. false false _485_ 0 7013 9 Not in stock true See design considerations of the strong RBS and spacer. false Jorge Eduardo Buend??a Buend??a component2092403 1 BBa_K360031 component2092400 1 BBa_J23101 component2092405 1 BBa_E0040 annotation2092405 1 BBa_E0040 range2092405 1 61 780 annotation2092400 1 BBa_J23101 range2092400 1 1 35 annotation2092403 1 BBa_K360031 range2092403 1 44 54 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K360031_sequence 1 aggaaacagct BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K360143_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaggaaacagcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z