LOCUS       BBa_K361011             1484 bp    DNA     linear       27-Dec-2024
DEFINITION  Rci recombinase system
ACCESSION   BBa_K361011
VERSION     BBa_K361011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_S04509
     CDS             227..1381
                     /label=Rci recombinase
     CDS             227..1381
                     /label=BBa_K361000
     stem_loop       1397..1416
                     /label=T7 TE
     misc_feature    1423..1423
                     /label=stop
     polyA_site      1417..1430
                     /label=polya
     misc_feature    1390..1430
                     /label=BBa_B0012
     terminator      1390..1430
                     /label=BBa_B0012
     stem_loop       1451..1473
                     /label=stem_loop
     misc_feature    1439..1484
                     /label=BBa_B0011
     terminator      1439..1484
                     /label=BBa_B0011
     terminator      1390..1484
                     /label=BBa_B0014
     misc_feature    227..1484
                     /label=BBa_S04524
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc cgtctccacg
      241 catccgtaaa atgtccctgt cacgcgcact ggataagtac ctgaaaacag tttctgttca
      301 caagaaaggg catcaacagg agttttaccg gagcaatgtt atcaagcgat atcccattgc
      361 ccttcggaat atggacgaaa taacaaccgt tgatattgct acatacagag acgttcgttt
      421 agcagaaatc aacccccgaa cgggtaaacc cattacaggt aatactgtac gtcttgaact
      481 cgcccttctg tcatctctgt tcaatattgc tcgtgttgaa tggggaacct gtcgtactaa
      541 cccggttgaa ctggttcgca agccgaaagt atcctcggga cgagatcgcc ggctaacgtc
      601 ttcagaagaa cgtcgccttt ctcgctattt ccgcgaaaaa aatctgatgt tgtatgtcat
      661 tttccatctt gcccttgaga cagccatgcg gcagggcgaa atactggcct tacgttggga
      721 gcacattgat ttgcgccacg gtgtggctca tttacctgaa accaaaaacg gtcactcacg
      781 ggatgttcct ctgtccagac gtgcccgtaa ctttcttcag atgatgcccg ttaatctcca
      841 cggcaatgtt tttgattaca ccgcatccgg ctttaaaaat gcctggagaa tagccacaca
      901 acgacttcgc atcgaggacc tgcattttca cgatctacgg catgaagcaa taagccgctt
      961 cttcgaactg ggtagcctga atgtaatgga gattgctgca atatcaggac atcgttccat
     1021 gaatatgctg aaacggtata ctcatcttcg tgcatggcaa ctggtcagta agcttgatgc
     1081 ccgccggcgg cagacacaaa aagtggcagc atggtttgtg ccgtatcctg cccatatcac
     1141 gaccatcgat gaagaaaatg ggcagaaagc gcatcgtatt gagatcggtg attttgataa
     1201 ccttcacgtc actgccacaa cgaaagagga agcagttcac cgcgccagtg aggttttgtt
     1261 gcgtacactg gccattgcag cacagaaagg cgaacgtgtc ccatctcccg gagcgttacc
     1321 tgttaacgac cctgattaca ttatgatttg ccctctgaac ccgggcagca ccccgctgta
     1381 atactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag
     1441 agaatataaa aagccagatt attaatccgg cttttttatt attt
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