BBa_K361011
type: ComponentDefinition
persistent identity: BBa K361011
display id: BBa_K361011
version: 1
was generated by: iGEM to SBOL conversion
description: Rci recombinase system
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: The promotor is come from lacI regulated one: BBa_R0010 The ribosome binding site is come from the defines RBS efficiency equal to one : BBa_B0034 The terminator is come from a doble terminator with bidirectional termination: BBa_B0014
owned by: james
owned by: myers
mutable description: This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.
discontinued: false
dominant: false
experience: experience/None
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This is the optimal system for Rci site-specific recombinase production.
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_S04509
component: BBa_S04524
sequence annotation: BBa_S04509
sequence annotation: BBa_S04524
sequence: https://synbiohub.org/public/igem/BBa_K361011_sequence/1

BBa_S04509
type: Component
persistent identity: component2095262
display id: component2095262
version: 1
top level: BBa_K361011
definition: BBa_S04509
access: public

BBa_S04524
type: Component
persistent identity: component2095272
display id: component2095272
version: 1
top level: BBa_K361011
definition: BBa_S04524
access: public

BBa_S04509
type: SequenceAnnotation
persistent identity: annotation2095262
display id: annotation2095262
version: 1
top level: BBa_K361011
location: https://synbiohub.org/public/igem/BBa_K361011/annotation2095262/range2095262/1
component: BBa_S04509

https://synbiohub.org/public/igem/BBa_K361011/annotation2095262/range2095262/1
type: Range
persistent identity: range2095262
display id: range2095262
version: 1
top level: BBa_K361011
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_S04524
type: SequenceAnnotation
persistent identity: annotation2095272
display id: annotation2095272
version: 1
top level: BBa_K361011
location: https://synbiohub.org/public/igem/BBa_K361011/annotation2095272/range2095272/1
component: BBa_S04524

https://synbiohub.org/public/igem/BBa_K361011/annotation2095272/range2095272/1
type: Range
persistent identity: range2095272
display id: range2095272
version: 1
top level: BBa_K361011
direction: direction/reverse
start: 227
end: 1484
orientation: inline

BBa_S04509
type: ComponentDefinition
persistent identity: BBa S04509
display id: BBa_S04509
version: 1
was generated by: iGEM to SBOL conversion
description: R0010:B0034
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:14:41Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 6123
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04509_sequence/1

BBa_B0034
type: Component
persistent identity: component2090743
display id: component2090743
version: 1
top level: BBa_S04509
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2090735
display id: component2090735
version: 1
top level: BBa_S04509
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2090735
display id: annotation2090735
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
type: Range
persistent identity: range2090735
display id: range2090735
version: 1
top level: BBa_S04509
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2090743
display id: annotation2090743
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
type: Range
persistent identity: range2090743
display id: range2090743
version: 1
top level: BBa_S04509
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_S04524
type: ComponentDefinition
persistent identity: BBa S04524
display id: BBa_S04524
version: 1
was generated by: iGEM to SBOL conversion
description: K361000:B0014
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:14:41Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0014
component: BBa_K361000
sequence annotation: BBa_K361000
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_S04524_sequence/1

BBa_B0014
type: Component
persistent identity: component2095190
display id: component2095190
version: 1
top level: BBa_S04524
definition: BBa_B0014
access: public

BBa_K361000
type: Component
persistent identity: component2095183
display id: component2095183
version: 1
top level: BBa_S04524
definition: Rci
access: public

BBa_K361000
type: SequenceAnnotation
persistent identity: annotation2095183
display id: annotation2095183
version: 1
top level: BBa_S04524
location: https://synbiohub.org/public/igem/BBa_S04524/annotation2095183/range2095183/1
component: BBa_K361000

https://synbiohub.org/public/igem/BBa_S04524/annotation2095183/range2095183/1
type: Range
persistent identity: range2095183
display id: range2095183
version: 1
top level: BBa_S04524
direction: direction/reverse
start: 1
end: 1155
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2095190
display id: annotation2095190
version: 1
top level: BBa_S04524
location: https://synbiohub.org/public/igem/BBa_S04524/annotation2095190/range2095190/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_S04524/annotation2095190/range2095190/1
type: Range
persistent identity: range2095190
display id: range2095190
version: 1
top level: BBa_S04524
direction: direction/reverse
start: 1164
end: 1258
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

Rci
type: ComponentDefinition
persistent identity: BBa K361000
display id: BBa_K361000
version: 1
was generated by: iGEM to SBOL conversion
description: Rci site-specific recombinase
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
mutable provenance: It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1
owned by: james
owned by: myers
mutable description: This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition. After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.
discontinued: false
dominant: true
experience: experience/Works
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The gene is come from muliple inversion systems in R64 plasmid and there is no further modification. Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process.
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Rci recombinase
sequence: https://synbiohub.org/public/igem/BBa_K361000_sequence/1

Rci recombinase
type: SequenceAnnotation
persistent identity: annotation2095181
display id: annotation2095181
version: 1
top level: Rci
location: https://synbiohub.org/public/igem/BBa_K361000/annotation2095181/range2095181/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K361000/annotation2095181/range2095181/1
type: Range
persistent identity: range2095181
display id: range2095181
version: 1
top level: Rci
direction: direction/forward
start: 1
end: 1155
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K361011_sequence/1
type: Sequence
persistent identity: BBa K361011 sequence
display id: BBa_K361011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04509_sequence/1
type: Sequence
persistent identity: BBa S04509 sequence
display id: BBa_S04509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04524_sequence/1
type: Sequence
persistent identity: BBa S04524 sequence
display id: BBa_S04524_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K361000_sequence/1
type: Sequence
persistent identity: BBa K361000 sequence
display id: BBa_K361000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccgtctccacgcatccgtaaaatgtccctgtcacgcgcactggataagtacctgaaaacagtttctgttcacaagaaagggcatcaacaggagttttaccggagcaatgttatcaagcgatatcccattgcccttcggaatatggacgaaataacaaccgttgatattgctacatacagagacgttcgtttagcagaaatcaacccccgaacgggtaaacccattacaggtaatactgtacgtcttgaactcgcccttctgtcatctctgttcaatattgctcgtgttgaatggggaacctgtcgtactaacccggttgaactggttcgcaagccgaaagtatcctcgggacgagatcgccggctaacgtcttcagaagaacgtcgcctttctcgctatttccgcgaaaaaaatctgatgttgtatgtcattttccatcttgcccttgagacagccatgcggcagggcgaaatactggccttacgttgggagcacattgatttgcgccacggtgtggctcatttacctgaaaccaaaaacggtcactcacgggatgttcctctgtccagacgtgcccgtaactttcttcagatgatgcccgttaatctccacggcaatgtttttgattacaccgcatccggctttaaaaatgcctggagaatagccacacaacgacttcgcatcgaggacctgcattttcacgatctacggcatgaagcaataagccgcttcttcgaactgggtagcctgaatgtaatggagattgctgcaatatcaggacatcgttccatgaatatgctgaaacggtatactcatcttcgtgcatggcaactggtcagtaagcttgatgcccgccggcggcagacacaaaaagtggcagcatggtttgtgccgtatcctgcccatatcacgaccatcgatgaagaaaatgggcagaaagcgcatcgtattgagatcggtgattttgataaccttcacgtcactgccacaacgaaagaggaagcagttcaccgcgccagtgaggttttgttgcgtacactggccattgcagcacagaaaggcgaacgtgtcccatctcccggagcgttacctgttaacgaccctgattacattatgatttgccctctgaacccgggcagcaccccgctgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z